Role of peroxisome proliferator-activated receptor (PPAR)-α gene in Dyslipidemia (original) (raw)

Rawal Medical Journal, 2020

Abstract

Objective: To identify the role of peroxisome proliferator-activated receptor ( PPAR)-α gene in Dyslipidemia. Methodology: This experimental study was carried out at Center for research in experimental and applied medicine (CREAM) Department of Biochemistry and Molecular Biology, Army Medical College, Rawalpindi from December 20, 2016 to June 8, 2017. The study comprised of three groups; Group one included subjects who were healthy, group two was comprised of diabetic without dyslipidemia and group three included diabetics with dyslipidemia. Blood was collected and Primers specific to PPAR-α were designed on the basis of sequence available on website Primer BLAST. RNA was extracted with in first six hours of sample collection using Gene JET RNA purification kit (Thermo Scientific). For first strand cDNA synthesis Revert Aid Premium was utilized. Polymerase chain reaction was used for the isolation, amplification or detection of specific nucleotide sequences in genomic DNA. PCR produ...

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