Recombinant production of rhesus θ-defensin-1 (RTD-1) using a bacterial expression system (original) (raw)

Defensins are antimicrobial peptides that are important in the innate immune defense of mammals. In contrast to mammalian a-and b-defensins, rhesus y-defensin-1 (RTD-1) comprises only 18 amino acids stabilized by three disulfide bonds and an unusual backbone cyclic topology. In this work we report for the first time the recombinant expression of the fully folded y-defensin RTD-1 using a bacterial expression system. This was accomplished using an intramolecular native chemical ligation in combination with a ...