Effect of diode laser biostimulation compared to Teriparatide on induced osteoporosis in rats: an animal study from Egypt (original) (raw)
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Possible Role of Low Level Laser Therapy on Bone Turnover in Ovariectomized Rats
Endocrine Regulations, 2010
The aim of this study was to assess the effect of low level laser therapy (LLLT) on bone turnover markers in ovariectomized rats. Thirty adult female albino rats were used in this study and divided into three groups: Group (1); 10 sham-operated rats served as controls group (2); 10 bilateral ovariectomized rats (OVX), and group ; 10 OVX rats exposed to LLLT. LLLT was applied on the neck and shaft of femur, 5 times/week for 8 weeks. The dose applied on each point was 1000 Hertz, 5 Watts for 30 seconds with a total dose of 15 mJoule/cm 2 .At the end of the experiment, blood samples were collected and sera were separated for determination of serum calcium (Ca), inorganic phosphorus (Pi), osteocalcin and alkaline phosphatase (ALP). In addition, a 24 hour urine sample was also collected from each rat for the determination of urinary calcium, phosphorous, and deoxypyridinoline(DPD)/creatinine. Results showed significant increase in serum Ca, Pi, ALP, osteocalcin, and significant decrease in U-DPD/creatinine in LLLT exposed group as compared to the other two groups. Bone morphological results revealed increase in calcium deposition and alkaline phosphatase of femoral bones of LLLT exposed group in comparison to sham-operated and OVX rats. Using software image analysis showed increased osteoblast numbers, decreased osteoclast numbers and increased compact bone thickness in LLLT exposed group. Significant positive correlations were obtained between osteoblast numbers and serum Ca, Pi, ALP, and osteocalcin in LLLT exposed group, while a significant negative correlation was noticed with U-DPD. In conclusion, the use of LLLT was found to be effective in enhancing bone formation, decreasing bone resorption in the osteoporotic OVX rats. Further studies are necessary to investigate the effect of different parameters of LLLT as wave length, duration, and also numbers of sessions. The potential use of LLLT in postmenopausal women with osteoporosis is needed to be verified.
Lasers in Medical Science, 2011
The present study aimed to evaluate the effects of LLLT (660-and 808-nm wavelengths) on the process of repairing bone defects induced in the femurs of female rats submitted to ovariectomy. Bilateral ovariectomies were performed on 18 female Wistar rats, which were divided into control and irradiated groups after the digital analysis of bone density showed decreased bone mass and after standardized drilling of the femurs. The irradiated groups received 133 J/cm 2 of AsGaAl (660-nm) and InGaAlP (880-nm) laser radiation. The animals were euthanized on days 14 and 21 after the bone defects were established.
Lasers in Medical Science, 2015
Osteoporosis (OP) increases vertebral fragility as a result of the biomechanical effects of diminished bone structure and composition. This study has aimed to assess the effects of pulsed wave low-level laser therapy (PW LLLT) on cancellous bone strength of an ovariectomized (OVX-d) experimental rat model and a glucocorticoid-induced OP (GIOP) experimental rat model. There were four OVX-d groups and four dexamethasone-treated groups. A group of healthy rats was used for baseline evaluations. The OVX-d rats were further subdivided into the following groups: control rats with OP, OVX-d rats that received alendronate, OVX-d rats treated with PW LLLT, and OVX-d rats treated with alendronate and PW LLLT. The remaining rats received dexamethasone and were divided into four groups: control, alendronate-treated rats, laser-treated rats, and laser-treated rats with concomitant administration of alendronate. PW LLLT (890 nm, 80 Hz, 0.972 J/cm 2) was performed on the spinal processes of the T12, L1, L2, and L3 vertebras. We extracted the L1 vertebrae and submitted them to a mechanical compression test. Biomechanical test findings showed positive effects of the PW LLLT and alendronate administration on increasing bending stiffness and maximum force of the osteoporotic bones compared to the healthy group. However, laser treatment of OVA-d rats significantly increased stress high load compared to OVA-d control rats. PW LLLT preserved the cancellous (trabecular) bone of vertebra against the detrimental effects of OV-induced OP on bone strength in rats compared to control OV rats.
Experimental Gerontology, 2012
Background and objective: The goal of this study was to investigate the effects of low level laser therapy (LLLT) in osteoporotic rats by means of subjective histopathological analysis, deposition of collagen at the site of fracture, biomechanical properties and immunohistochemistry for COX-2, Cbfa-1 and VEGF. Material and methods: A total of 30 female Wistar rats (12 weeks-old, ±250 g) were submitted to ovariectomy (OVX). Eight weeks after the OVX, a tibial bone defect was created in all animals and they were randomly divided into 3 groups (n = 10): control bone defect group (CG): bone defects without any treatment; laser 60 J/cm 2 group (L60): animals irradiated with LLLT, at 60 J/cm 2 and laser 120 J/cm 2 group (L120): animals irradiated with LLLT, at 120 J/cm 2 . Results: In the laser treated groups, at both fluences, a higher amount of newly formed bone was evidenced as well as granulation tissue compared to control. Picrosirius analysis demonstrated that irradiated animals presented a higher deposition of collagen fibers and a better organization of these fibers when compared to other groups, mainly at 120 J/cm 2 . COX-2, Cbfa-1 or VEGF immunoreactivity was detected in a similar manner either 60 J/cm 2 or 120 J/cm 2 fluences. However, no differences were shown in the biomechanical analysis. Conclusion: Taken together, our results support the notion that LLLT improves bone repair in the tibia of osteoporotic rats as a result of stimulation of the newly formed bone, fibrovascularization and angiogenesis.
Effects of 830-nm Laser Light on Preventing Bone Loss after Ovariectomy
Photomedicine and Laser Surgery, 2006
Objective: The aim of this study was to investigate the effects of low-level laser therapy (LLLT; infrared, 830 nm) on the bone properties and bone strength of rat femora after ovariectomy (OVX). Background Data: Osteoporosis affects 30% of postmenopausal women, and it has been recognized as a major public health problem. Based on the stimulatory effects of LLLT on proliferation of bone cells, we hypothesized that LLLT would be efficient in preventing bone mass loss in OVX rats. Methods: Forty female rats were divided into four groups: sham-operated control (SC), OVX control (OC), sham-operated irradiated at a dose of 120 J/cm 2 (I120), and OVX irradiated at a dose of 120 J/cm 2 (O120). Animals were operated at the age of 90 days. Laser irradiation was initiated 1 day after the operation and was performed three times a week, for 2 months. Femora were submitted to a biomechanical test and a physical properties evaluation. Results: Maximal load of O120 was higher than in control groups. Wet weight, dry weight, and bone volume of O120 did not show any difference when compared with SC. Conclusion: The results of the present study indicate that LLLT was able to prevent bone loss after OVX in rats. However, further studies are needed to investigate the effects of different parameters, wavelengths, and sessions of applications on OVX rats.
Laser therapy in bone repair in rats: analysis of bone optical density
Acta ortopedica brasileira, 2014
To investigate, by digital radiology, the bone regeneration process in rats submitted to femoral osteotomy and treated with low power laser therapy. Forty-five Wistar rats were subjected to transverse osteotomy of the right femur and divided randomly into three experimental groups (n = 15): animals not treated with laser therapy G (C), animals that received laser therapy with λ: 660nm G (660nm) and animals that received laser therapy with λ: 830nm G (830nm). Animals were sacrificed after 7, 14 and 21 days. The bone calluses were evaluated by digital X-ray at 65 kVp, 7mA and 0.032 s exposures. The values obtained were submitted to variance analysis (ANOVA) followed by the Tukey-Kramer test. The significance level adopted was 5%. The groups G (C), G (660nm), and G (830nm) at the 7(th) day showed a significant bone development, with p <0.0116; the groups G (C), G (660nm), and G (830nm) at the 14(th) day showed values of p <0.0001; at the 21(st) day,a higher degree of bone repair ...
Analysing the Effect of Low Level Laser Therapy on Bone Histomorphometry in Rats
Recent Research Advances in Biology Vol. 11, 2021
The aim of this study was to assess the histological effect of Low Level Laser Therapy (LLLT) (904 nm) on the repair of standardized bone defects on the femur of rats. Sixty male wistar rats were assigned into two equal groups. Group (A: laser group) and group (B: control group). A surgical fracture was done in middle third of femur of all rats. In group (A) a continuous wave 904 nm infrared laser was applied at dose 4 j/cm 2 at fracture site immediately post operative for 7 sessions, each session was 5 minutes. The animals were killed by over dose of general anesthesia on the 15th,30th and 45th days after surgery, The specimens were processed and stained with Hematoxylin-eosin (H/E), special stain Masion trichrome and analyzed by light microscopy. The descriptive analysis of histological imaging showed greater degree of new bone formation, osteoblastic surface and collagen fiber in the irradiated group when compared with the control group. Based on the obtained results, this study concluded that LLLT was efficient in promoting bone healing, and increasing new bone formation in the process of surgically fractured femur in animal study.
Biosilicate® and low-level laser therapy improve bone repair in osteoporotic rats
Journal of Tissue Engineering and Regenerative Medicine, 2011
The aim of this study was to investigate the effects of a novel bioactive material (Biosilicate ) and low-level laser therapy (LLLT) on bone fracture consolidation in osteoporotic rats. Forty female Wistar rats were submitted to ovariectomy (OVX) to induce osteopenia. Eight weeks after surgery, the animals were randomly divided into four groups of 10 animals each: a bone defect control group (CG); a bone defect filled with Biosilicate group (BG); a bone defect filled with Biosilicate and irradiated with LLLT at 60 J/cm 2 group (BG60); and a bone defect filled with Biosilicate and irradiated with LLLT at 120 J/cm 2 group (BG120). Bone defects were surgically performed on both tibias. The size of particle used for Biosilicate was 180-212 µm. Histopathological analysis showed that bone defects were predominantly filled with the biomaterial in specimens treated with Biosilicate. LLLT with either 60 or 120 J/cm 2 was able to increase collagen, Cbfa-1, VGEF and COX-2 expression in the circumjacent cells of the biomaterial. A morphometric analysis revealed that the Biosilicate + laser groups showed a higher amount of newly formed bone. Our results indicate that laser therapy improves bone repair process in contact with Biosilicate as a result of increasing bone formation, as well as COX-2 and Cbfa-1 immunoexpression, angiogenesis and collagen deposition in osteoporotic rats.
Journal of Biomedical Optics, 2011
We investigate the effects of a novel bioactive material (Biosilicate®) and low-level laser therapy (LLLT), at 60 J/cm2, on bone-fracture consolidation in osteoporotic rats. Forty female Wistar rats are submitted to the ovariectomy, to induce osteopenia. Eight weeks after the ovariectomy, the animals are randomly divided into four groups, with 10 animals each: bone defect control group; bone defect filled with Biosilicate group; bone defect irradiated with laser at 60 J/cm2 group; bone defect filled with Biosilicate and irradiated with LLLT, at 60 J/cm2 group. Laser irradiation is initiated immediately after surgery and performed every 48 h for 14 days. Histopathological analysis points out that bone defects are predominantly filled with the biomaterial in specimens treated with Biosilicate. In the 60-J/cm2 laser plus Biosilicate group, the biomaterial fills all bone defects, which also contained woven bone and granulation tissue. Also, the biomechanical properties are increased in the animals treated with Biosilicate associated to lasertherapy. Our results indicate that laser therapy improves bone repair process in contact with Biosilicate as a result of increasing bone formation as well as indentation biomechanical properties.
Effect of Low Level Laser Therapy on Bone Histomorphometry in Rats
Life Science Journal, 2011
Abstract: The aim of this study was to assess the histological effect of Low Level Laser Therapy (LLLT) (904 nm) on the repair of standardized bone defects on the femur of rats. Sixty male wistar rats were assigned into two equal groups. Group (A: laser group) and group (B: control ...