Sugar beet pulp as a carrier for Lactobacillus paracasei in lactic acid fermentation of agro-industrial waste (original) (raw)
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Journal on processing and energy in agriculture, 2016
Distillery stillage is abundant industrial waste with a great potential for utilization as a substrate in production of valuable biobased products. Processing of distillery stillage on cost effective way can significantly decrease the price of bioethanol as alternative fuel and provide a considerable benefit to the environment. In this paper, combined potato stillage and sugar beet molasses substrate is evaluated for integrated lactic acid and probiotic biomass production by Lactobacillus rhamnosus ATCC 7469. Waste substrate based on potato stillage and sugar beet molasses enabled lactic acid and biomass production with maximal volumetric lactic acid productivity of 1.11 g L-1 h-1 and maximal number of viable L. rhamnosus ATCC 7469 cells of 1.1×10 9 CFU mL-1. Residue after removal of lactic acid could be recommended as probiotics and betaine-enriched animal feed.
Industrial Crops and Products, 2018
In this study, the production of lactic acid (LA) on sugar beet molasses enriched potato stillage by immobilized Lactobacillus paracasei NRRL B-4564 was examined. Three agro-industrial materials, such as sunflower seed hull (SSH), brewers' spent grain (BSG), and sugar beet pulp (SBP) were studied as carriers for cell immobilization. The carriers were physically characterized in terms of water adsorption index (WAI), critical humidity point (CHP) and porosity. Further, the stability and efficiency of the immobilized biocatalysts were evaluated in repeated batch fermentation of molasses enriched potato stillage and compared with free cell system. A strong cell attachment onto agro-industrial supports allowed easy separation from the fermentation media and efficient biocatalyst reuse in five successive batch cycles. The highest cell number attached on the support surface during the fermentation was detected for material with higher WAI and lower CHP. Porosimetry measurements showed that the attachment of L. paracasei cells to support materials and overall LA productivities achieved with different immobilized biocatalysts were not determined by the support porosity and surface morphology, but with the material characteristics such as electrostatic charge, chemical composition and hydrophilicity. LA productivity of 1.48 g/L h, maximal LA concentration of 80.10 g/L and average yield coefficient of 0.97 g/g were achieved in fermentation of molasses enriched potato stillage using SBP as a support material, followed by BSG and SSH. The studied approach showed to be an interesting alternative strategy for increasing the LA productivities on low-cost and abundant substrate.
Electronic Journal of Biotechnology, 2011
Background: Owing to the growing interest in biofuels, the concept of a biorefinery where biomass is converted to a variety of useful products is gaining ground. We here present how distillery waste is combined with a by-product from a sugar production, molasses, to form a medium for the growth of Lactobacillus plantarum with yields and biomass densities comparable with conventional industrial media. Such approach enables a cost-effective utilization of the problematic wastewater from ethanol and a by-product from sugar production. It is the first approach that attempts to find low-cost media for the production of Lactobacillus plantarum biomass. Results: This study suggests that sieved wheat stillage enriched by adding 1.77 g/l yeast extract and 10% molasses (v/v), with NH4OH used for pH adjustment, may be used as a media for large-scale cultivation of L. plantarum. Such composition of the medium permits a high density of lactic acid bacteria (1.6 x 10 10 cfu/ml) to be achieved. Conclusions: The use of a fermentation medium consisting of distillery wastewater and molasses to obtain value-added products (such as LAB biomass and lactic acid) is a possible step for classical ethanol production to move towards a biorefinery model production in which all by and waste products are utilized to increase produced values and reduce waste production. This enables a cost-effective utilization of the problematic wastewater from ethanol and sugar production.
Journal of Chemical Technology & Biotechnology, 1997
Process variables were optimized for the production of lactic acid from pretreated beet molasses by L actobacillus delbrueckii IFO 3202 for batch and continuous fermentations. In the batch fermentation, maximum yields (95É4% conversion, 77É1% e †ective) and maximum lactic acid volumetric productivity (4É83 g dm~3 h~1) was achieved at 45¡C, pH 6É0, 78É2 g dm~3 sugar concentration with 10 g dm~3 yeast extract. Various cheaper nitrogen sources were replaced with yeast extract on equal nitrogen bases in batch fermentation. Of all the nitrogen sources tested, yeast extract yielded the highest and malt sprouts yielded the second highest level of lactic acid. In the continuous fermentation, maximum lactic acid (4É15%) was obtained at a dilution rate of 0É1 h~1. Maximum volumetric lactic acid productivity (11É20 g dm~3 h~1) occurred at D \ 0É5 h~1 dilution rate.
Lactic acid production on a combined distillery stillage and sugar beet molasses substrate
Journal of Chemical Technology & Biotechnology, 2015
BACKGROUND: Utilization of wastes and industrial by-products for production of valuable bio-based chemicals has economic and environmental advantages. The aim of this study was to investigate utilization of two industrial by-products, distillery stillage and sugar beet molasses combined as a substrate for lactic acid and biomass production. A selection of the most appropriate lactic acid bacteria that could effectively utilize this waste substrate was performed. In addition, the effect of initial sugar concentration on lactic acid production and growth of lactic acid bacteria on the combined waste substrate was evaluated. RESULTS: Lactobacillus paracasei NRRL B-4564 was selected as the most promising for lactic acid production on distillery stillage and sugar beet molasses. The highest lactic acid productivity of 1.42 g L −1 h −1 and yield of 0.91 g g −1 were achieved on stillage/molasses media at initial sugar concentration of 56.74 g L −1. The highest number of L. paracasei cells at 5.3 × 10 9 CFU mL −1 was achieved at initial sugar concentration of 78.22 g L −1. CONCLUSION: Combined distillery stillage and sugar beet molasses could provide valuable nutrients for growth of fastidious lactic acid bacteria and enable efficient lactic acid production, while processing industrial waste in this way has great environmental relevance.
Feasibility of exhausted sugar beet pulp as raw material for lactic acid production
Journal of the Science of Food and Agriculture, 2020
BACKGROUNDExhausted sugar beet pulp pellets (ESBPP), a sugar industry by‐product generated after sugar extraction in the sugar production process, have been used as a raw material for lactic acid (LA) production via hydrolysis and fermentation by Lactobacillus casei. To design a more cost‐effective process, simultaneous saccharification and fermentation (SSF) of ESBPP is proposed in the present study. The effects of pH control, nutrient supplementation and solid addition in fed‐batch SSF on lactic acid production were investigated.RESULTSThe highest LA concentration (26.88 g L–1) was reached in fed‐batch SSF at a solid/liquid loading of 0.2 g mL–1, with pH control (by adding 30 g L–1 CaCO3 to the medium) and nutrient supplementation (by adding 20 mL of MRS medium per 100 mL of buffer). Under these conditions, a maximum productivity of 0.63 g L−1 h−1 was achieved, which is 2.7 times higher than that attained in the control experiment (SSF inoculated at time 0 h). However, a slightly ...
Optimization of lactic acid production from beet molasses by Lactobacillus delbrueckii NCIMB 8130
World Journal of Microbiology & Biotechnology, 2002
Production of lactic acid from beet molasses by Lactobacillus delbrueckii NCIMB 8130 in static and shake flask fermentation was investigated. Shake flasks proved to be a better fermentation system for this purpose. Substitution of yeast extract with other low cost protein sources did not improve lactic acid production. The maximum lactic acid concentration was achieved without treatment of molasses. A Central Composite Design was employed to determine the maximum lactic acid concentration at optimum values for the process variables (sucrose, yeast extract, CaCO3). A satisfactory fit of the model was realized. Lactic acid production was significantly affected both by sucrose–yeast extract and sucrose–CaCO3 interactions, as well as by the negative quadratic effects of these variables. Sucrose and yeast extract had a linear effect on lactic acid production while the CaCO3 had no significant linear effect. The maximum lactic acid concentration (88.0 g/l) was obtained at concentrations for sucrose, yeast extract and CaCO3 of 89.93, 45.71 and 59.95 g/l, respectively.
Food waste management-a cheap source of lactic acid produced by Lactobacillus sp
2016
The most common and important chemical compound used in pharmaceutical, cosmetic, chemical and food industry is lactic acid. There have been various attempts made to produce lactic acid efficiently from inexpensive raw materials. The main objective of present study was to produce lactic acid from cheap food waste such as potato peels, orange peels and mango peels as substrate. A total of 35 isolates were screened for Lactobacillus spp. On the basis of temperature and pH optimization, 4 bacterial isolates (5SA, 21SA, 22SA, 32SA) were selected for further study and fermentation. The highest lactic acid production, 12.23 g L was obtained for mango peels where as for orange peels it was 11.98 g L for 21SA isolate. Isolates 5SA and 22SA produced 13.08 g L and 12.54 g L, respectively, lactic acid for potato peels at the 3rd of fermentation. For mixed peel waste, 32SA isolate was able to produce maximum 11.56 g L lactic acid after fermentation at the end of 3rd day. Thus it shown that lact...
Biomass Conversion and Biorefinery, 2022
Production of lactic acid from lignocellulosic biomass is becoming more popular as a way to solve the problem associated with first-generation biomass. In the present study, the optimum fermentation parameters for maximum lactic acid production from khat waste biomass by using immobilized Lactobacillus plantarum were investigated. The effect of immobilizing the cell on the yield of lactic acid has been investigated, and the yield obtained from immobilized Lactobacillus plantarum cell is 32.78% higher than the free cells. The effects of three process parameters (incubation temperature, pH, and incubation time) on lactic acid yield were investigated. The optimization of lactic acid yield and fermentation parameters was done via response surface methodology with a central composite design. Based on the analysis, the optimum fermentation parameters were found to be incubation temperature of 42.3 °C, incubation time of 40.0 h, and pH of 6.18. Under these conditions, the maximum lactic acid yield obtained was 23.05 g/L. The experimental yield of lactic acid (22.98 ± 0.10 g/L) is in smooth agreement with the predicted one (23.05 g/L), indicating the fitness of the quadratic model used. Generally, the findings of the study suggest that utilization of khat waste as a potential carbohydrate source and immobilization of Lactobacillus plantarum cells for lactic acid production is worthful.
Lactic acid is an organic acid produced by Lactobacillus strains and being used in many industries. The present study is aimed to elucidate the ability of lactic acid production with different Lactobacillus strains using various agro waste substrates. These Lactobacillus were isolated from various dairy products. Out of 30 isolates, 15 isolates were identified as Lactobacillus strains by morphological and physiological characterization. These strains were subjected to Solid state fermentation and Submerged fermentation by utilizing the peels of Apple, Cassava, Mosambi, Orange and Pineapple. SSF with Mosambi peel showed a higher yield of lactic acid from Lactobacillus sp LAB 23.Optimum conditions such as temperature, pH, nitrogen sources, mineral salts, inoculum concentration and fermentation time were evaluated for SSF and SmF. In SSF, the optimum culture conditions for the maximum lactic acid production were temperature 37°C, pH 6.5, 2.5% yeast extract as a nitrogen sources, 8% Calicum carbonate, 0.3% magnesium sulphate as mineral salts, 4 % inoculums concentration, substrate concentration 8g, 72 h for fermentation. In SmF, the optimum culture conditions for maximum lactic acid production were temperature 40°C, pH7.5, 3.5% yeast extract as a nitrogen sources, 9% Calicum carbonate, 0.4% magnesium sulphate as mineral salts, 8% inoculums concentration, 10 mL substrate concentration and fermentation time 96 h. The present study suggests that the Mosambi peel is an appropriate agro waste substrates for high yield of lactic acid under optimized conditions. Thus, the Mosambi peel could be used as a good substrate in various industries.