Purification and characterization of the alpha form of rat plasma kallikrein (original) (raw)

Brazilian Journal of Medical and Biological Research, 1987

Abstract

A four-step procedure was used to purify rat plasma kallikrein (RPK) with a relative molecular mass (Mr) of 87 kD (obtained both by gel filtration and SDS-PAGE), which indicates the purification of an alpha (intact) kallikrein, in contrast to previously described RPK preparations which had lower Mr (beta or degraded form). RPK is a neutral (pI 6.7) serine proteinase glycoprotein (15% carbohydrates) and contains (residues/mol): galactose (27), N-acetylglucosamine (24), mannose (13), glucose (13) and fucose (7). This purified alpha form of RPK has properties very similar to those of pure human and bovine kallikreins.

Y. Michelacci hasn't uploaded this paper.

Let Y. know you want this paper to be uploaded.

Ask for this paper to be uploaded.