Synthesis of gold nanoparticles by the fungus Aspergillus niger and its efficacy against mosquito larvae (original) (raw)
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Fungus Generated Novel Nanoparticles: A New Prospective for Mosquito Control
International journal of recent scientific research, 2013
This work was to evaluate the efficacies of the nanoparticles (NPs) of silver (Ag) and Gold (Au) against the major tropical mosquitoes. In the present study, the Ag and Au NPs were synthesized by using the cell free extract of Fusarium oxysporum f.sp. pisi fungus. The bioreduction of AgNPs and AuNPs was monitored by ultraviolet-visible spectroscopy, and the AgNPs and AuNPs obtained were characterized by transmission electron microscopy and scanning electron microscopy. The synthesized AgNPs and AuNPs were spherical particles ranging in size from 20-40 nm (AgNPs) and 2-10 nm (AuNPs). Further, these synthesized NPs were also tasted as larvicides and pupicides against the larvae and pupae of Cx. quinquefasciatus, An. stephensi and Ae. aegypti. The efficacy test was performed at different concentrations for a period of different hours by the probit analysis. The maximum efficacy was observed in synthesized AgNPs against the larvae of Ae. aegypti (LC50 8, 6, 4, LC90 12.30, 12.58, 11.48, ...
Advances in Nanoparticles, 2013
Here, we have synthesized the silver nanoparticles (AgNPs) by using the soil fungus Aspergillus niger 2587. The results recorded from UV-vis spectrophotometer and transmission electron microscopy (TEM) support the biosynthesis and characterization of AgNPs. The synthesized silver nanoparticles have also been tested against the larvae and pupae of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti. The efficacy test was performed at different concentrations for a period of different hours by the probit analysis. The larvae of Cx. quinquefasciatus have shown the 100% mortality to the synthesized AgNPs after 1 h of exposure, while the larvae of An. stephensi and Ae. aegypti were found less susceptible to the synthesized AgNPs. The pupa of Ae. aegypti has shown the efficacy LC 50 4, LC 90 12 and LC 99 19 ppm after 2 h of exposure of the synthesized AgNPs, while, the pupae of Cx. quinquefasciatus and An. stephensi were found less susceptible to the synthesized AgNPs. By this approach, it is suggestive that this rapid synthesis of nanoparticles would be proper for developing a biological process for mosquito control.
Journal of Fungi
Herein, silver nanoparticles (Ag-NPs) were synthesized using an environmentally friendly approach by harnessing the metabolites of Aspergillus niger F2. The successful formation of Ag-NPs was checked by a color change to yellowish-brown, followed by UV-Vis spectroscopy, Fourier transforms infrared (FT-IR), Transmission electron microscopy (TEM), and X-ray diffraction (XRD). Data showed the successful formation of crystalline Ag-NPs with a spherical shape at the maximum surface plasmon resonance of 420 nm with a size range of 3–13 nm. The Ag-NPs showed high toxicity against I, II, III, and IV instar larvae and pupae of Aedes aegypti with LC50 and LC90 values of 12.4–22.9 ppm and 22.4–41.4 ppm, respectively under laboratory conditions. The field assay exhibited the highest reduction in larval density due to treatment with Ag-NPs (10× LC50) with values of 59.6%, 74.7%, and 100% after 24, 48, and 72 h, respectively. The exposure of A. aegypti adults to the vapor of burning Ag-NPs-based ...
Green Nanoparticles for Mosquito Control
The Scientific World Journal, 2014
Here, we have used the green method for synthesis of silver and gold nanoparticles. In the present study the silver (Ag) and gold (Au) nanoparticles (NPs) were synthesized by using the aqueous bark extract of Indian spice dalchini (Cinnamomum zeylanicum) (C. zyelanicumorC. verumJ. Presl). Additionally, we have used these synthesized nanoparticles for mosquito control. The larvicidal activity has been tested against the malaria vectorAnopheles stephensiand filariasis vectorCulex quinquefasciatus. The results were obtained using UV-visible spectrophotometer and the images were recorded with a transmission electron microscope (TEM). The efficacy tests were then performed at different concentrations and varying numbers of hours by probit analysis. The synthesized AgNPs were in spherical shape and average sizes (11.77 nm AgNPs and 46.48 nm AuNPs). The larvae ofAn. stephensiwere found highly susceptible to the synthesized AgNPs and AuNPs than theCx. quinquefasciatus. These results suggest...
Insecticidal activity of bio-silver and gold nanoparticles against
2016
A laboratory experiment was conducted to evaluate the impact of silver aqueous solution, gold aqueous solution, pungamoil, pungamoil-based silver nanoparticles (PO-AgNPs) and pungamoil-based gold nanoparticles (PO-AuNPs), vijayneem against Pericallia ricini larval mortality, development and fecundity and gravimetric analyses. The results showed that Ag + aqueous solution as well as pungamoil-based gold nanoparticles caused more mortality than commercial neem insecticide (vijayneem). Aqueous solutions of Ag + and Au + drastically reduced the body weight of P. ricini larvae. PO-AgNPs and POAuNPs treatment drastically enhanced the food consumption, assimilation but reduced the conversion, subsequently affecting growth. Distinct difference was noticed between PO-AgNPs and PO-AuNPs treatments in larval, pupal and adult developmental periods, fecundity and hatchability. It is recommended to integrate PO-AgNPs and PO-AuNPs in pest management; however, field evaluations and bio-safety asses...
A Review on Nanoparticles in Mosquito Control - A Green Revolution in Future
Mosquitoes are the potential vectors of many diseases, including malaria, filariasis, dengue, brain fever, etc. There is an urgent need to check the proliferation of the population of vector mosquitoes in order to reduce vector borne diseases by appropriate control methods. Mosquito control is of serious concern in developing countries like India due to the lack of general awareness, development of resistance, and socioeconomic reasons. Nanotechnology, a promising field of research opens up in the present decade and is expected to give major impulses to technical innovations in a variety of industrial sectors in the future. Over the past few decade, nanoparticles of noble metals such as silver exhibited significantly distinct physical, chemical and biological properties from their bulk counterparts. Nano-size particles of less than 100 nm in diameter are currently attracting increasing attention for the wide range of new applications in various fields of industry. Presently, there is a need for increasing the efforts to develop newer and effective methods to control mosquito vectors. The existing chemical and biological methods are not as effective as in earlier period owing to different technical and operational reasons. In particular, this present paper focused on potential role of nanoparticles in mosquito control.
Efficacy of metal nanoparticles as a control tool against adult mosquito vectors: A review
Frontiers in tropical diseases, 2022
Presently, there is a need to develop effective and novel modes of control for mosquitoes, which remain a key driver of infectious disease transmission throughout the world. Control methods for these vectors have historically relied on a limited number of active ingredients (AIs) that have not experienced significant change in usage since the mid-20 th century. The resulting development of widespread insecticide resistance has consequently increased the risk for future vector-borne disease outbreaks. Recently, metal nanoparticles have been explored for potential use in mosquito control due to their demonstrated toxicity against mosquitoes at all life stages. However, the majority of studies to date have focused on the larvicidal efficacy of metal nanoparticles with few studies examining their adulticidal potential. In this review, we analyze the current literature on green synthesized metal nanoparticles and their effect on adult mosquitoes.
Journal of Cluster Science, 2019
The present investigation focuses on the larvicidal property of gold and silver nanoparticles (AuNPs and AgNPs) that were synthesized using the supernatant of Photorhabdus luminescens strain KPR-8B from nematode. The synthesized AuNPs and AgNPs were characterized through UV-visible spectrophotometer, X-ray diffraction analysis, Fourier transform infrared spectroscopy, dynamic light scattering and high-resolution transmission electron microscopic analyses. The characterization studies confirmed the spherical shape and size (14-46 nm) of AuNPs and AgNPs. The synthesized AuNPs and AgNPs were evaluated against 4th instar larvae of three mosquitoes, Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus. The highest larval mortality was observed after 24 h from the KPR-8B derived AuNPs against A. aegypti with the LC 50 and LC 90 values of 5.04 and 12.65 lg/ml, respectively.
Malaria is considered a dreadful mosquito-borne infectious disease of human beings caused and spread by biting of the female mosquito Anopheles stephensi infected with a parasitic protozoan Plasmodium falciparum. Continuous application of chemicals/synthetic insecticides for vector control causes various problems such as resistant mechanism of mosquito, toxicity to nontarget aquatic organisms and disturbance to the microbial community of the soil. Currently, green synthesized nanoparticles are being employed in various biological processes including insect and pest control. The present investigation focused on the mosquito-larvicidal property of Turbinaria ornata-mediated gold nanoparticles (To-AuNPs) and its boiled aqueous extract (To-AE) against the malarial vector A. stephensi. The recorded lethal concentration (LC50 and LC90) values (μg/ml) of To-AE and To-AuNPs against fourth instar larvae of A. stephensi were 37.77 and 159.55 and 12.79 and 78.70, respectively. The To-AuNPs were characterized through UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), high-resolution transmission electron microscopy (HRTEM), energy dispersive x-ray spectroscopy (EDX), zeta potential and dynamic light scattering (DLS) method. The presently synthesized gold nanoparticles through the single-step, eco-friendly method is a potentially effective mosquitocidal agent.
“Eco-friendly and Economic approach of Green Synthesized Nanoparticles as Aedes Mosquito Control.”
Journal of emerging technologies and innovative research, 2019
World’s 40% population is at risk of dengue which is transmitted by Aedes aegypti and Aedes albopictus. Many measures for Aedes control are taken but still mosquitoes find their way to breed and transmit diseases. Insecticide use is been considered effective for emergency mosquito control and many more insecticides are under trials but mosquitoes usually develop resistance against these chemical formulations. In recent years green synthesized nanoparticles are proved to be potential targets against larvae, pupa and adult form of mosquito. This paper reviews the various developed nanoparticles during and their potentiality in Aedes control. Larvicidal activity of nanoparticles is given for I instars, II instars, III instars, IV instars and pupa differently. Nanoparticles are fabricated from many medicinal plants extract and some are very much effective against mosquito control. This is an eco friendly approach for mosquito control with low risk of resistance development.