Substrate specificity of recombinant Streptomyces clavuligerus deacetoxycephalosporin C synthase (original) (raw)

The study delves into the high-level expression of the deacetoxycephalosporin C synthase (DAOCS) gene from Streptomyces clavuligerus in Escherichia coli, exploring its substrate specificity and enzymatic kinetics. Results demonstrate effective conversion of penicillin N and several related substrates to deacetoxycephalosporin C, with a notable distinction from bifunctional synthase activity observed in other species. Insights into substrate specificity and enzyme behavior enhance understanding of cephalosporin biosynthesis, potentially informing future biotechnological applications.