Setting up a Diagnostic Molecular Laboratory (original) (raw)

2014

Abstract

Create a successful workflow for PCR ◦ Earlier years – contamination of PCR rxn,s with amplification product from previous PCR was a potential problem To Combat contamination – ◦ Three separate rooms Preparing the reaction Amplification Analysis of amplified products New instruments – closed systems – less contamination risk Facilities still need to be well designed No inflexible guidelines in molecular lab design – primary emphasis is avoidance of contamination with each step of the workflow

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