Hepatoprotective effect of herbal drug on CCl(4) induced liver damage (original) (raw)
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The hepatoprotective action of ten herbal extracts in CCl4 intoxicated liver
Phytotherapy Research, 2005
The effect of ten phytotherapeutic products on CCl4 intoxicated liver in albino male Wistar rats was investigated. Biochemical parameters, including serum transaminase activity (GPT and GOT), histoenzymological measurements (lactate dehydrogenase, LDH; succinate dehydrogenase, SDH, cytochromoxidase, CyOx; Mg2+-dependent adenosine triphosphatase, ATP-ase) and histochemical (Sudan black) and histological examinations (haematoxylin-eosin staining) of the liver were investigated. Some positive effects such as the reduction of hepatocytolysis and steatosis, and a return to normal values of the activity of some enzymes in the following plants: Chrysanthemum balsamita, Echinacea pallida, Calendula officinalis and Corylus avelana were obtained. Copyright © 2005 John Wiley & Sons, Ltd.
GSC Biological and Pharmaceutical Sciences, 2022
Traditional healers combine four medicinal plants (Cochlospermum tinctorium, Terminalia macroptera, Leptadenia hastata and Commiphora Africana to treat hepatitis in Burkina Faso. The aimed was to evaluate the hepato-curative activity of lyophilized aqueous decoction (LAD) and hydroethanolic macerate (LHM) of plant extracts on CCl4-induced hepatitis in rats. We assessed the acute toxicity and scavenging activity of the 2, 2-Diphenyl-1-picrylhydrazyl (DPPH). Hepato-curative activity study included nine groups with five rats each. We used rats as followed: group 1 as neutral controls, group 2 as negative controls, and the other groups were experimental groups. Rats in groups 2-9 received a single dose (1 mL/kg) of CCl4 in intraperitoneal injection to induce hepatitis. We fed orally the rats for seven consecutive days with sylimarin in group 3, LAD and LHM respectively in groups 4-6 and groups 7-9 by 400, 200 and 100 mg/kg/day. This study revealed LAD and LHM had a LD50> 2000 mg/kg a...
EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF MARKETED HERBAL DRUG PREPARATION
The aim of the present study is to evaluate the protective effect of marketed herbal preparation (Duliv) against carbon tetrachloride (CCl4) induced liver damage in Albino rats. Administration with Duliv Tablet (100mg/kg p.o.)and Duliv Syrup 0.64 ml/100 gm for 15 days significantly reduced the impact of CCl4 toxicity on the serum markers of liver damage, SGPT,SGOT, albumin,protein , cholesterol and alkaline phosphatase.The histopathological studies in the liver of rats also supported that Duliv tablet and Duliv syrup markedly reduced the toxicity of CCl 4 and preserved the histoarchitecture of the liver tissue to near normal. Thus, the results suggest that Duliv preparation acts as a potent hepatoprotective agent against CCl4 induced hepatotoxicity in rats.
Indian journal of experimental biology, 2001
Abhrak bhasma is a commonly used ayurvedic drug against many diseases including hepatitis. It is tested in albino rats using a model of hepatitis induced by a single dose of CCl4 (3 ml/kg body wt). Different doses of abhrak bhasma (10, 20, 30 and 40 mg/kg body wt) were tested to decide the dose related hepatoprotective efficacy. The centrolobular necrosis induced by single dose of CCl4 was reduced significantly by abhrak bhasma (10 mg) and liver histology was also protected by 20 mg dose. Liver acid lipase activity was lowered, while alkaline and lipoprotein lipase activities were elevated due to treatment of single dose of CCl4. Abhrak bhasma counteracted the action of CCl4 on liver lipolytic enzymes. CCl4 did not alter the kidney histologically. Activities of three lipases of rat kidney (acid, alkaline and lipoprotein lipases) were reduced by CCl4 treatment and were reversed by administration of abhrak bhasma. Acid lipase activity of rat adipose tissue was reduced by CCl4 treatmen...
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Objective: The leaves of Caesalpinia bonduc (CB) have been used against various disorders in folk medicine including the liver disorders. Earlier, we have shown the hepatoprotective effect of CB in acute hepatotoxicity model. The present study was designed to evaluate the anti-hepatotoxic and anti-fibrotic effect of the aqueous leaf extract of CB on CCl4 (carbon tetrachloride) induced chronic hepatotoxicity/fibrosis in Wistar rats. Methods: Animals were divided into three groups namely; preventive, curative and prophylactic, which was further subdivided into four groups each: Group I-untreated control, group II-CCl4 control, group III-CB+CCl4 and group IV-silymarin+CCl4. The aqueous extract of CB/silymarin was administered orally once, daily for eight weeks in the curative group and for four weeks in preventive and prophylactic groups respectively. The chronic liver damage/fibrosis was induced by intraperitoneal injection of CCl4 twice a week, for four weeks in preventive and prophylactic groups and for eight weeks in the curative group. Blood samples were collected for assaying serum biochemical parameters, and the livers were excised and processed for histology. Results: The data showed that supplementation of aqueous leaf extract of CB along with CCl4 significantly reduced the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase(ALP), total bilirubin(TB) and prothrombin time(PT) thus further restoring the total protein(TP) and albumin(ALB) in preventive, curative and prophylactic groups when compared to CCl4 control. Significant improvement in the microscopic structure of the liver further confirmed the hepatoprotective effect of aqueous extract of CB over the liver injury and fibrosis induced by CCl4 in rats. Conclusion: The study, therefore, suggests that aqueous extract of CB might provide a novel and alternative approach for treating the chronic hepatotoxicity/liver fibrosis.
Effect of Selected Plant Parts Extracts on Liver Injuries Induced by CCl4 in vitro
The objective of this study is to highlight the potential effects of selected plant parts extracts [Sweet violet blossoms (SVB), marjoram leaves (ML), red onion skin (ROS) and orange peel (OP)] individually or to work together to improve the liver injuries induced by CCl4 in vitro. Fish liver homogenate and human lymphocytes cultures were used as in vitro biological model systems. The data indicated that CCl4 induced many adverse cytotoxic, immunotoxic and genotoxic effects including lysosomal and mitochondrial dysfunctions, cell membrane integrity and decreasing in protease activity in fish liver homogenate and DNA damage in human lymphocytes cultures. Co-treatment of liver homogenate with CCl4 and the tested selected plant parts extracts as well as their mixture by concentration 0.75% exhibited many therapeutic effects through decreasing the rates of all those adverse effects. That decreasing rates in different toxic effects was depending on the type of the plant parts applied. The highest therapeutic effect was recorded for the mixture of the selected plant parts extracts (OP+SVB+ROS+ML by equal parts) followed by ROS, ML, OP and SVB, respectively. Such data indicated that there has been considerable interest in the role of complementary and alternative medicines for the prevention/treatment of liver injury induced in humans by many environmental toxins.