In vitro/in vivo effect of Citrus limon (L. Burm. f.) juice on blood parameters, coagulation and anticoagulation factors in rabbits (original) (raw)
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Present study was performed on two combination doses of Citrus limon and Punica granatum i.e. 0.4ml/kg Citrus limon +5ml/kg Punica granatum (CPJ-1) and 0.2ml/kg Citrus limon +8ml/kg Punica granatum (CPJ-2) in healthy rabbits. CPJ-1 and CPJ-2 both showed significant increase in red blood cell count, hemoglobin, bleeding time and anticoagulation factors like protein C and thrombin antithrombin complex levels. While CPJ-1 cause significant increase in thrombin time and platelet count. However Fb was significantly decreased by CPJ-1. These results suggest that Citrus limon and Punica granatum has phytochemicals and other essential nutrients that possesses an anti-thrombin component, prevent thrombosis and plays cardio protective role.
Animals
The current study examined the influence of Citrus limon (dry lemon) on the hemato-biochemical profiles, and antioxidant indices of growing rabbits. Forty-eight growing New Zealand White rabbits (age, eight weeks; weight, 1543.33 ± 25 g) were allocated into three groups (16 animals each), the first group was (control) fed a basal diet, whereas the second and third groups were supplemented with dried lemon, 1% or 2% DLP, respectively. A GC-MS analysis of more than 27 active constituents was performed. Feed conversion efficiency was (p < 0.05) better with diets containing 1% or 2% dry lemon, compared to the control group. Hematological indexes were increased significantly with the addition of DLP compared to those in the control group. Adding 1% or 2% dry lemon to rabbit diet increased (p < 0.05) enzymatic and non-enzymatic antioxidant activities (TAC, SOD, GSH, GST, and CAT) in serum and liver tissues. Taken together, these data reveal the advantages and antioxidant effects of ...
Journal of Scientific Research and Reports
Aims: To identify viable phytomedicines traditionally employed for the treatment of malaria in Kenya that could be developed into antimalarial agents. Study Design: Quantitative analysis of antiplasmodial activities and brine shrimp bioassays were carried out using standard procedures. The experiment was set in duplicate for each concentration of the drug and average IC50 determined. Place and Duration of Study: Seven indigenous plants: Achyranthes aspera, Heinsiacrinita, Bridelia cathartica, Citrus limon, Microglossapyrifolia, Vernoniaglabra and Carissa edulis obtained from Kilifi and Homa-Bay counties in Kenya were evaluated for their anti-Plasmodium falciparum potential. Collection of samples and analysis took about three months from April 2018. Methodology: Both Chemical and aqueous crude extraction methods were carried out to identify the most active extracts against P. Falciparum and then isolate pure active phytochemicals. Pure compounds were subjected to Nuclear Magnetic Res...
In this present study, the leaf extracts of Citrus hystrix were subjected to a comparative evaluation of preliminary phytochemical screening, membrane stabilizing activity, thrombolytic activity and In-vitro antioxidant activity. The thrombolytic and membrane stabilizing activities were assessed by using human erythrocyte and the results were compared with standard streptokinase (SK) and standard anti-inflammatory drug, acetyl salicylic acid (ASA), respectively. For thrombolytic activity and membrane stabilizing activity ethanolic leaf extract exhibited clot-lysis value of 13.69% against standard SK (37.43 %) and highest percentage of hemolytic value of 74.40% against standard ASA (93.24%) respectively. DPPH free radical scavenging activity, Cupric Reducing Antioxidant Capacity (CUPRAC), Total Phenolics Content, Total Flavonoids Content, Nitric oxide scavenging assay and total antioxidant capacity were done in the present study. In case of in-vitro antioxidant evaluation all extract forms showed a significant positive results. Phytochemical investigation suggested the presence of alkaloids, carbohydrates, flavonoids, glycosides, phenols, steroids and tannins.
Ethnopharmacological relevance of Citrus limon (L.) Burm. f. as adjuvant therapy
nnals of Phytomedicine , 2023
Citrus limon (L.) Burm. f. (C. limon), commonly known as lemon, is a plant with a rich ethnopharmacological history and a wide range of bioactive compounds. This review explores the ethnopharmacological relevance of C. limon in management of diseases. The use of C. limon in traditional medicine systems, such as Ayurveda and Traditional Chinese Medicine, dates back centuries, highlighting its significance as a therapeutic agent. C. limon possesses diverse pharmacological properties, including antioxidant, anti-inflammatory, antimicrobial, antidiabetic, anticancer, and hepatoprotective activities, attributed to its various phytochemicals, such as flavonoids, limonoids, coumarins, terpenes, and phenolic compounds. These properties make C. limon a promising candidate for complementary or adjuvant therapy alongside conventional treatments. Traditional knowledge and anecdotal evidence have long supported using C. limon for various ailments. Modern scientific research has begun elucidating the molecular mechanisms behind its pharmacological effects. Studies have identified bioactive compounds from C. limon and investigated their interactions with molecular targets, shedding light on their potential therapeutic applications. The studies have also provided insights into the safety, efficacy, and dosage considerations of C. limon based interventions. This comprehensive review analyzes the ethnopharmacological relevance of C. limon, including its traditional uses, phytochemical composition, and biological activities. It explores the mechanisms of action of C. limon focusing on various disease conditions where C. limon has shown promise pharmacological activities. By consolidating the available evidence, this review is a valuable resource for researchers, healthcare professionals, and practitioners seeking to integrate C. limon into evidence-based treatment strategies. The findings highlight the potential of C. limon as a supportive treatment alongside conventional therapies, contributing to improved patient outcomes and enhanced holistic healthcare approaches. Further research is warranted to fully understand the therapeutic potential and optimize the use of C. limon in adjuvant therapy.
Antiplatelet Activity of Hydroalcoholic Extracts of Citrus Sinensis Peels
2015
The major flavonoids contained in sweet orange fruit peels (SOP) , hesperidin and rutin , are able to inhibit platelet aggregation, a biological activity that could provide the products derived from this part of the plant with an antithrombotic potential. This work was aimed to assess the possible antiplatelet activity of SOP extracts in 50 %and 70 % ethanol (SOPE50 and SOPE70, respectively). The turbidimetric method of platelet aggregation (PA) was used to evaluate the in vitro inhibition of human platelet-rich plasma (PRP) response to ADP, collagen, epinephrine and arachidonic acid (AA), as well as the ex vivo reduction of the reactivity of rat PRP to ADP with acetylsalicylic acid (ASA) as a reference drug. SOPE50 significantly inhibited ADP-, collagenand epinephrine-induced PA and was ineffective on platelet reactivity to AA. SOPE70 partially inhibited ADPinduced PA and was ineffective against collagen. Platelet reactivity to ADP was significantly inhibited after a single intrape...