High-Throughput Screening of 75 Euphorbiaceae and Myrtaceae Plant Extracts for In-Vitro Antitumor and Pro-Apoptotic Activities on Human Tumor Cell Lines, and Lethality to Brine Shrimp (original) (raw)

2013

Abstract

Objective: Various approaches are used for discovering cytotoxic anticancer therapeutics from plants. In this study 75 extracts representing 47 plant species belonging to families Euphorbiaceae and Myrtaceae were screened by three in-vitro bioassays, to detect extracts with possible antitumor activity. Methods: The methanol plant extracts were screened over three bioassays viz., cytotoxicity on HepG2 human hepatocellular carcinoma cell line assessed by MTT method, pro-apoptotic activity on HCT116 human colon carcinoma cell line measured by the increase of M30 neo-epitope apoptosis biomarker through sandwich ELISA, as well as brine shrimp lethality (BSL) bioassay. Results: 54 extracts were active in at least one bioassay. 43 extracts (57%) scored cytotoxic on HepG2, compared to 32 (43%) pro-apoptotic extracts in HCT116-M30 assay and 18 (24%) lethal extracts in BSL test. Out of the 43 cytotoxic extracts 15 (20%) possessed pro-apoptotic activity, which may be considered the ideal candi...

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