Purification of AGE-BSA on Affi-Gel-Blue (original) (raw)

2011

Abstract

Three peaks were observed: the first one corresponds to highly glycated BSA which did not bind to the matrix, the second one corresponds to the less glycated BSA which bound moderately, and the third peak corresponds to the stronger bound BSA. The first peak was the only one to be further analyzed. The arrows indicate the buffer used at each stage.<b>Copyright information:</b>Taken from "Glycation does not modify bovine serum albumin (BSA)-induced reduction of rat aortic relaxation: The response to glycated and nonglycated BSA is lost in metabolic syndrome"Glycobiology 2008;18(7):517-525.Published online 5 May 2008PMCID:PMC2430009.

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