Very High Salivary Streptococcus Mutans Predicts Caries Progression in Young Children (original) (raw)
Related papers
Journal of Clinical and Experimental Dentistry, 2015
Objectives: Determination of the relative amounts of mutans streptococcus in both saliva and plaque and to study its correlation with dental caries in children. Study Design: The study comprised of 60 children aged 3-8 years divided into 2 groups (30 children in each): Group A-Children with more than 4 carious teeth and Group B-Children without caries. Saliva and plaque was collected from children of both the groups with the help of Dentocult SM strip test kit (Orion Diagnostic). Following incubation, mutans streptococcus scores (from 0 to 3) in each individual was evaluated and compared between both the groups. Results: On comparing the two groups, mean ± SD of saliva score and plaque score was 2.40 ± 0.675 and 2.40 ± 0.621 respectively in group A, whereas it was 0.60 ± 0.498 and 0.83 ± 0.531 in children of group B showing a significant correlation (p = < 0.001) between mutans streptococci scores in both saliva and plaque and dental caries experience. Conclusions: There is a direct and strong co-relation between the salivary and plaque mutans streptococcus counts and caries activity in children aged 3-8 years.
2019
Introduction: Streptococcus mutans plays a significant role in causation of caries. Transmission of S. mutans can be vertical or horizontal from mother to child and mainly occur during the first 2.5 years of age. In this article, we have emphasized on the transmission of S. mutans from parents to their 18 months and 3-year-old children. The aim of this study was to find out correlation between salivary mutans Streptococcus count and dental caries experience among preschool children and their parents. Materials and Methods: The study comprised randomly selected 50 father-mother-child pairs who were residing at Gandhi Nagar, a village in Indore district. Of 50 children, 25 were of 18 months and the other 25 were of 36 months of age. At early morning, the saliva samples of each pair were obtained before their brushing. The parents were interviewed in accordance with a structured questionnaire. The vital statistics of children and their parents were recorded. The clinical examination of...
World journal of dentistry, 2024
Aim: This case-control study aimed to evaluate the levels of mutans streptococci in primary caries in children and permanent caries in adolescents, with levels of mutans streptococci in caries-free children and adolescents in correlation with the decayed, missing, and filled permanent teeth (DMFT) scores. The oral health behavior of these groups was also studied. Materials and methods: The sample size comprised 60 subjects-15 children having caries in primary dentition and 15 caries-free children in the age range of 2-5 years, 15 adolescents having caries in permanent dentition, and 15 caries-free adolescents in the age range of 12-15 years. The oral health behavior and dietary practices were recorded according to the World Health Organization (WHO) oral health questionnaire. The decayed, missing, and filled primary teeth (dmft)/DMFT index was calculated using WHO caries diagnostic criteria. Plaque samples were collected aseptically from the tooth sites, pooled, and inoculated on mitis salivarius agar (MSA). The total mutans streptococci count (CFU/mL) was then determined. The data were analyzed using paired t-tests and analysis of variance (ANOVA). Results: Mutans streptococci in the caries group in children were 1.35 ± 1.99 CFU/mL. This was significantly higher (p < 0.01) compared to mutans streptococci in the caries group in adolescents, which was 0.8 ± 0.14 CFU/mL. The study shows high levels of mutans streptococci in the primary caries group with a high DMFT score correlating with increased sugar consumption and, to a lesser extent, with tooth brushing. Conclusion: In the present study, it is observed that mutans streptococci are more predominant in caries of children than in the caries of adolescents. Clinical significance: Caries research has focused largely on mutans streptococci as the main etiological pathogen of dental caries, but its impact on different stages of dentition has not been fully understood. Caries susceptibility associated with mutans streptococci is significantly high in the primary dentition stage. Therefore, the acquisition/transmission of mutans streptococci to prevent caries should be targeted in childhood.
2013
Background: To find out if there is an association between levels of salivary Mutans Streptococci (MS) of mothers and salivary MS and Caries experience of their Children. Materials and Methods: A cross sectional study was designed among 180 mother-child pair attending Vanivilasa Children Hospital, Bangalore, India. Caries was diagnosed using WHO criteria. Ninety children with Early Childhood Caries (ECC) with their mothers were included in case group and 90 caries free children with their mothers were included in the control group. Whole non-stimulated saliva was collected from mothers and children and cultured for MS. Colonies were counted and compared. Data was analyzed using SPSS version 10. Chi-square test and t-test were used to find differences between groups. P value of <0.05 was considered as significant. Results: Among 180 children, 80 of them had a high MS count. Among them, 60 (75%) had ECC and only 20 (25%) were caries free. This difference was significant with P <...
Journal of Medical Microbiology, 2005
Streptococcus mutans and Streptococcus sobrinus are known to be associated with the development of dental caries. In this study these bacteria were detected in pre-school children (each with primary dentition, age range 3-5 years, n ¼ 60) using a PCR method, and then their presence was compared with the incidence of dental caries over a 1-year period. Plaque samples were collected from all erupted tooth sites using a sterile toothbrush. Dental examinations at the beginning of the study (baseline) and after 1 year were also performed to determine decayed, missing, filled teeth (dmft) scores using WHO caries diagnostic criteria. The prevalences of S. mutans and S. sobrinus across all the subjects were 61 . 7 % and 56 . 6 %, respectively; 13 subjects (21 . 7 %) were positive for S. mutans alone, 10 (16 . 6 %) were positive for S. sobrinus alone and 24 (40 . 0 %) were positive for both S. mutans and S. sobrinus, whereas 13 (21 . 7 %) were negative for both S. mutans and S. sobrinus. dmft scores of subjects positive for both S. mutans and S. sobrinus at baseline and after 1 year were significantly higher than of those positive for S. mutans alone at the same stages (P , 0 . 01 and P , 0 . 001, respectively). The caries incremental increase was also significantly greater in those with both bacteria detected (P , 0 . 05). Our results indicate that pre-school children harbouring both S. mutans and S. sobrinus have a significantly higher incidence of dental caries than those with S. mutans alone.
Mutans Streptococci counts from saliva and its protein profile in early childhood caries
Interventional Medicine and Applied Science
Aim: This study aims to analyze the number Mutans Streptococci (MS) and its protein profile from the saliva of early childhood caries (ECC) and caries-free subjects. Methods: MS counts were cultured from saliva samples, and the protein profile of MS was determined from ECC and caries-free subjects. The number of colonies were counted, and the protein bands with the molecular weight of 13, 29, 39, 41.3, 74, and 95 kDa were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis method. Results: We found that the number of colonies from saliva of ECC patients was higher than those caries-free (22.20 × 106 CFU/ml vs. 19.16 × 106 CFU/ml, p < 0.05). There are higher expression frequencies in protein 29, 39, 41.3, and 74 kDa of MS in ECC than caries-free subjects. Conclusions: There is the higher number of MS colonies and difference of MS protein profile isolated from saliva among children with ECC and caries-free counterparts.
Salivary streptococcus mutans count and caries outcome - a systematic review
2010
OBJECTIVE: To answer whether an increased salivary S. mutans count is associated with higher caries. METHODS: Six databases were searched for articles (4 February 2009). Inclusion criteria: (i) titles/abstracts relevant to topic; (ii) published in English, Portuguese, Spanish; (iii) prospective cohort study; (iv) at least 2 groups/cohorts are investigated, for which a baseline salivary mutans count and a caries outcome after a period of time is reported; (v) caries outcome reported as computable datasets. RESULTS: Fifteen from the initial 134 articles were selected. From these, 5 were rejected and 10 articles reporting on 30 separate datasets accepted. Owing to heterogeneity, no metaanalysis was undertaken. Cohorts which differed statistically significantly (p<0.05) in their caries outcomes also differed largely in their salivary SM counts; cohorts with similar (p>0.05) caries outcomes differed little. Cohorts with S. mutans count >10 5 cfu/ml showed higher caries outcome. The results showed a 55% lower caries risk in subjects with S. mutans <5 x 10 5 cfu/ml (Relative Risk 0.45-95% CI 0.34, 0.59-p<0.00001). Potential bias may have influenced study results. CONCLUSION: Increased S. mutans counts are associated with higher caries outcomes. High quality cohort studies are needed to validate the current evidence.
Caries Research, 2008
The aim of this study was to examine the colonization of Streptococcus mutans and Streptococcus sanguinis in the oral cavity and the association with severe early childhood caries (S-ECC). Saliva and plaque samples were collected from 14 S-ECC children and 8 caries-free (CF) children. All S-ECC children were S. mutans positive; 100% of CF children and 93% of S-ECC children were S. sanguinis positive. The children’s caries severity was positively correlated with levels of S. mutans (p < 0.001), total oral streptococci (p < 0.01), total cultivable oral bacteria (p < 0.05), and children’s age (p < 0.05). Logistic regression analysis showed that the interaction of S. sanguinis with S. mutans was a significant factor associated with the caries status in children, suggesting that the relative levels of these two microorganisms in the oral cavity play an important role in caries development.
Acta Scientiarum. Health Science, 2018
The association between the genotypic diversity of Streptococcus mutans and risk factors for dental caries in children attending an educational program in the public sector was investigated. Twenty-one children (2-7 years old) who presented at least three risk factors were allocated into two groups: caries free (n = 12); with caries activity (n = 9). Initially, 210 isolates of S. Mutans were analyzed through AP-PCR and in the second intervention (after 12 months), new evaluation of risk factors and dmf-t index was carried out, followed by statistical analysis of the data (Simple Logistic Regression and Chi-square Test). There was an association between genotype diversity and caries (p = 0.05). It was found that 66,6% of the caries-free children had one genotype, while 77.7% in the group with caries had two or more genotypes. Having two or more genotypes increased by seven times the chance of injury. Genotypic diversity was associated with inadequate eating habits and oral hygiene pr...