Effects of system benzylaminopurine-adenine sulphate in combination with naphthalene acetic on in vitro regeneration and proliferation of pineapple (Ananas comosus (L.) Mill var. comosus) (original) (raw)
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MD2 pineapple is currently the most preferred variety on the international market pleasant aroma and high brix acidity ratio. Large quantities of planting material are needed to replace the existing Smooth Cayene and Sugar Loaf varieties commonly grown by farmers. Therefore in vitro procedures were developed as an alternate method to improve upon the multiplication rate of this variety. Clumped explants were cultured on MS supplemented with 30 g/l sucrose and various combination of 6-benzylaminopurine (BAP) and -naphthalene acetic acid (NAA) in solid or liquid cultures. Liquid cultures required 5.0 mg l BAP to significantly (P<0.05) 1 increase the multiplication rate by 2-or 5-fold compared to 7.5 mg l in solid cultures. When plantlets from 1 optimal treatments were transferred to liquid medium with high concentration (7.5-15 mg l ) of NAA there was 1 no root development. However, the transfer of one or two-month old cultured plantlets to solid MS medium supplemented with NAA or IBA alone or combination of NAA and IBA resulted in root production. The number of root produced per plantlet on a medium supplemented with a combination of NAA and IBA was comparably higher than when NAA or IBA alone were used. The increased multiplication rate of MD2 in vitro will serve as alternate source of planting materials for both subsistence and large-scale farmers in the pineapple industry.
Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants
Revista Brasileira de Fruticultura, 2010
This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regener...
Studies on Micropropagation of Pineapple (Ananas comosus L
The present study was conducted at the Tissue Culture Laboratory, Horticulture, Research Institute, Agricultural Research Center (ARC), Egypt during the period from December 2013 to March 2016 to investigate the effect of different type Murashige and Skooge (MS), Gamborge (B5) and Woody plant media (WPM) at four salt concentrations (Full, ¾, ½ and ¼) of culture media on micropropagability of pineapple (Ananas comosus L. var. Smooth Cayenne) during establishment stage. Shootlet proliferations were investigated at different concentrations of 6-benzyle amino purine (BAP) and kinitine (Kin) at 0.25, 0.5, 1.0 and 2.0 mg/l for each, during two successive subcultures. Finally, rooting capacity were studied by various concentrations of indole butyric acid (IBA) and indole acetic acid (IAA) at1.0, 2.0 and 3.0 mg/l on media containing activated charcoal. The rooted explants were transferred to greenhouse in peat moss and sand at various amounts 1:1, 1:2 and 2:1, respectively. The culture crowns were successfully disinfecting by Colorex 40% for 20 min with 77.77% survival and 100% free contamination. MS media at full strength was the best culture media showed shootlet number 1.75 shootlet/explant and shootlet length 2.25 cm with 9.25 leaf/shootlets. Among the different concentrations 2.0 mg/l BAP showed highest shoot proliferation of 56 and 42 shoots per explant at the first and second subculture, respectively. The longest shoot (5.5 and 5.5 cm) was produced in the two subcultures by the treatment combination of 0.25 mg/l BAP. The highest numbers of roots were produced by 1.0mg/l IAA were 10.67 roots/shootlet and the tallest length of roots were obtained for explants cultured on MS media containing IAA 3 mg/l. The individual rooted explants derived from plantlets were transferred to poly bags in the green house at mixture media after 7 days hardening in room temperature (28-30°C) and established plantlet was ready for planting.
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011
This study was conducted to evaluate the pineapple regeneration and shoot growth as affected by 6benzylaminopurine (BAP) at 2.0 mg/l and naphthalene acetic acid (NAA) at 0.2 mg/l in vitro. BAP and NAA at the concentration of 2.0 and 0.2 mg/l were used in this study. BAP at 2.0 mg/l significantly affected the production of shoots per explant, shoot length and weight. Total shoot length was higher in BAP (2 mg/l) than in control (MS medium without hormone) and NAA (0.2mg/l) after 10, 20, 30, 40, 50 and 60 days incubation period. Total shoot length was highest in BAP in all incubation periods. Total shoot weight was higher in BAP (2 mg/l) and lower in NAA (0.2 mg/l) as compared to MS medium without hormone. The results showed that BAP at the concentration of 2 mg/l was effective for pineapple shoot growth and development.
African Journal of Plant Science, 2014
Aseptic cultures of pineapple 'smooth cayenne' were established from shoot tip explants. They were initiated on Murashige and Skoog (MS) basal medium with vitamins supplemented with various combinations of 6-benzylaminopurine (BAP) (0, 0.1, 0.2, 0.3 and 0.4 mg L-1) and Gelrite [1.0 g L-1 (semiliquid), 1.5 g L-1 (semi-solid) and 2.0 g L-1 (solid)]. Explants were transferred five weeks after in vitro initiation to semi-liquid medium containing higher concentrations of BAP (0, 0.5, 1.0, 1.5 and 2.0 mg L-1) for proliferation. Four sub-cultures were made at five weeks interval for twenty weeks in the proliferation medium. Established shoots were introduced into rooting medium containing either full (4.4 g L-1) or half (2.2 g L-1) strength MS basal medium with vitamins supplemented with 0, 0.5 mg L-1 BAP alone or in combination with 0, 0.9 and 1.8 mg L-1 α-naphthaleneacetic acid (NAA). Semi-liquid MS basal medium supplemented with 0.1 and 0.3 mg L-1 BAP gave the best regeneration results, producing the highest average shoot length (34.6 mm) and average number of shoot buds (2.4) per explants. Significant difference (p≤0.05) in average shoot number per explant was observed with semi-liquid MS basal medium supplemented with 1.5 mg L-1 BAP, producing the highest average shoot number (6.1) per explant at 5 weeks and this increased to 167.7 after 20 weeks. The half-strength MS basal medium without growth regulators or with 0.9 mg L-1 NAA gave the highest average root length (29.3 mm) and root number per shoot (7.9). The semi-liquid MS basal medium supplemented with low BAP (1.5 mg L-1) is a cost-effective method for in vitro propagation of pineapple. Half strength MS basal medium without hormones or with low NAA are also cost-effective methods for inducing roots in pineapple.
Micropropagation of Pineapple (Ananas Comosus L. Merr. 'Josapine')
Acta horticulturae, 2011
In vitro regeneration of Ananas comosus L. Merr. 'Josapine' (pineapple) in tissue culture system was carried out using crown tip meristem. In vitro regeneration was done by manipulation of plant growth regulators including 6-benzylaminopurine (BAP) and combinations of BAP and naphthalene acetic acids (NAA) through organogenesis. The plant growth regulators were applied in MS medium supplemented with 8.0 g/L agar and 30 g/L sucrose. The crown tip meristem was used as explants and the optimum sterilization protocol was established in this study. The explant was maintained in 16 hours light 8 hours dark, at room temperature 25±2°C. The result shows that the crown tip managed to induce shoots in different concentrations of BAP and also combinations of BAP and NAA. Optimum regeneration was obtained onto MS medium supplemented with 3.0 mg/L BAP and MS medium containing 1.0 mg/L BAP/0.5 mg/L NAA. The shoots derived from crown tip meristem were being optimized onto MS liquid medium treated with optimum plant growth regulators. The in vitro plantlet of 'Josapine' pineapple was successfully acclimatized into the soil.
Micropropagation of pineapple, Ananas comosus (L.) Merr
Protocols for Micropropagation of Woody Trees and Fruits, 2007
In vitro regeneration of Ananas comosus L. Merr. 'Josapine' (pineapple) in tissue culture system was carried out using crown tip meristem. In vitro regeneration was done by manipulation of plant growth regulators including 6-benzylaminopurine (BAP) and combinations of BAP and naphthalene acetic acids (NAA) through organogenesis. The plant growth regulators were applied in MS medium supplemented with 8.0 g/L agar and 30 g/L sucrose. The crown tip meristem was used as explants and the optimum sterilization protocol was established in this study. The explant was maintained in 16 hours light 8 hours dark, at room temperature 25±2°C. The result shows that the crown tip managed to induce shoots in different concentrations of BAP and also combinations of BAP and NAA. Optimum regeneration was obtained onto MS medium supplemented with 3.0 mg/L BAP and MS medium containing 1.0 mg/L BAP/0.5 mg/L NAA. The shoots derived from crown tip meristem were being optimized onto MS liquid medium treated with optimum plant growth regulators. The in vitro plantlet of 'Josapine' pineapple was successfully acclimatized into the soil.
Micropropagation and Growth of in Vitro Pineapple (Ananas Comosus L. Merr) in Iran
2014
Although, several pineapple micropropagation protocols have already been published, significant improvement could be achieved, if the stages of in vitro culture were better defined. Our work concerned several experiments aiming at the mass production of high quality plantlets. Tissue culture experiments were therefore conducted to develop rapid multiplication procedures for Ananas comosus L. (Merr). Terminal buds from suckers were treated with 0.025% (w/v) mercuric chloride for 2 minutes and placed in different media. Explants were transferred to MS medium supplemented with NAA (2 mgl -1 ) and BA ( 0, 1, 2, 3, 4, 5, 6, 7 mgl -1 ) and kept for 2-4 months under 16/8 h photoperiod (40 µmol m -2 s -1 ) and 25 ± 2oC. Results showed that higher multiplication rates for Ananas comosus L. were obtained with BA concentrations of 5 mgl -1 at 3 months. The in vitro proliferated shoots produced roots with maximum frequency (84%) on MS medium without growth regulator at 6 weeks intervals. Using ...
Direct and indirect plant regenerations of pineapple var. MD2 (Ananas comosus L.)
The variety MD2 of pineapple (Ananas comosus L.) was used in this study. This variety is highly demanded in the international market and known for possessing harvest quality with aroma, high sugar content (14% Brix), vitamins and longer shelf life. However, shortage of planting material has limited the production in Malaysia. In vitro mass propagation using the direct and indirect shoot proliferation techniques was tested on variety MD2. The plantlets were successfully initiated from sucker on solid MS basal medium containing 30 g/L sucrose, 0.1 g/L Myo-inositol and 3 mg/L BAP after one month of culture. The highest direct shoot tips regeneration was obtained on solid MS medium when added with 30 g/L sucrose, 0.1 g/L Myoinositol, 3 mg/L BAP and 1 mg/L NAA. Indirect shoot regeneration was obtained on medium containing 3 mg/L Zeatin after one month of culture. In average, 10 shoots were regenerated from approximately 1 gram of calli. The techniques can produce 100-200 number of plantlet within 4 to 6 months of culture, and ready for planting after 7 months of culture.