Effect of various plant growth regulators on in vitro plantlet regeneration from different explants of Citrus sinensis (L.) (original) (raw)

The present investigation was intended to establish an effective protocol for direct shoot regeneration as well as root induction of Citrus sinensis using seed germinated shoot tip and nodal segments. Surface sterilization of explants was attributed with NaOCl (Sodium hypochloride) in shoot tip and nodal segments for successful contamination free culture. For direct shoot proliferation, nodal segments and shoot tips showed effective results when it cultured aseptically on MS (Murashige and Skoog) medium containing different concentrations and combinations of BAP (Benzylamino purine), GA3 (Gibberellic acids), NAA (Naphthalene acetic acid) and IAA (Indole acetic acid) the best result was observed in the combination of 1.5mg/l BAP+ 0.2 mg/l GA3. The explants were inoculated on MS medium supplemented with 8g/l Agar and 30g/l sucrose, different combination and concentration of plant growth regulators was used for plantlet regeneration. Root induction has also done here using various concentration of IAA hormone. The highest number of root induction was found to be MS containing 2.0mg/l IAA. Induced roots were elongated properly at the same hormonal concentration after two weeks of inoculation. From the above findings, it is suggested that different hormonal concentration and combination has significant effect on the propagation of sweet orange plant. The established protocol would be helpful for direct plantlet regeneration as well as proliferation of this valuable fruit crop plants.