Comparison of five methods, including the PDM Epsilometer test (E test), for antimicrobial susceptibility testing of Pseudomonas aeruginosa (original) (raw)
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Journal of Clinical Microbiology, 1992
The antimicrobial susceptibilities of 100 clinical isolates ofPseudononas aeruginosa to gentamicin, amikacin, tobramycin, ticarcillin, piperacillin, and ceftazidime were determined by using the Sceptor system (BBL Microbiology Systems, Cockeysville, Md.), and the results were compared with those obtained using the National Committee for Clinical Laboratory Standards reference agar dilution method. Excellent correlation was observed for the aminoglycosides, with >95% agreement within 1 doubling dilution of the reference agar dilution MIC, while ticarcillin and piperacillin showed lower percent agreement values of 91 and 88%,
Antimicrobial drug susceptibility of clinical isolates of Pseudomonas aeruginosa
The Canadian veterinary journal. La revue veterinaire canadienne
Unlike other gram-negative bacterial pathogens, Pseudomonas aeruginosa is intrinsically insensitive to many antimicrobial drugs due to the low rate of passage of antibiotics across its outer membrane (1). Though antipseudomonal drugs, such as amikacin, gentamicin, and carbenicillin, are used against infections caused by this organism, comparatively less expensive drugs, such as tetracycline and neomycin, do have application in veterinary medicine (2,3). Monitoring of drug susceptibility trends may help to determine the possible use of a drug or a group of drugs in a specific animal species. Predominance of serotypes has been found to be different in one animal species compared to another, and an association between antimicrobial susceptibility and serotypes has been shown (5,6). Though individual antibiograms are necessary for successful therapy, a knowledge of general susceptibility patterns may be helpful in certain situations.
Objective: To deduce the resistance pattern of multidrug resistant P.aeruginosa isolates to aminoglycosides (Amikacin and Gentamicin) from clinical specimens obtained from a tertiary care hospital in Karachi, Pakistan Methods: A prospective crossectional study was undertaken from January 2014 to January 2015 at a private tertiary care hospital in Karachi. Collection of specimens was carried out from three different units across the city. Customary and precise bacteriological procedures were used to detect the clinical isolates. The isolates were cultured on chocolate and MacConkey agar. Utilizing the Kirby Bauer Disc diffusion method on Mueller-Hinton Agar the sensitivity patterns were deduced Results: A total of 1622 isolates of P. aeruginosa were cultured during the study's time frame spanning over a year. Cultures which were found to be positive were then tested against Amikacin and Gentamicin and their pattern of susceptibility were ascertained. Conclusions: MDR P. aeruginos...
International Journal of Infectious Diseases, 2008
Background: Tigecycline, a member of a new class of antimicrobials (glycylcyclines), has been shown to have potent broad spectrum activity against most commonly encountered species responsible for hospital acquired infections. Cross-resistance to several classes of antimicrobials is often seen in nosocomial pathogens. The T.E.S.T. program determined the in vitro activity of tigecycline against strains of Enterobacteriaceae cross-resistant to one or more of the following antimicrobials: amoxicillin-clavulanic acid, piperacillin-tazobactam, levofloxacin, ceftriaxone, cefepime, ampicillin, amikacin, minocycline, ceftazidime and imipenem. The isolates were collected from 335 investigational sties in 47 countries throughout 2004-2007. Methods: A total of 26,791 clinical Enterobacteriaceae were identified to the species level at each site and confirmed by the central laboratory. Minimum Inhibitory Concentrations (MICs) were determined by the local laboratory using broth microdilution panels. Antimicrobial resistance was interpreted according to CLSI breakpoints with TIG susceptible and resistant breakpoints defined as = 8 mcg/ml, respectively.
Pseudomonas is a group of bacteria found in soil, water, skin flora and most man-made environments throughout the world. Pseudomonas infections may cause disease, generalized inflammation and sepsis. The targeted organism for this organism are the lungs, the urinary tract, and kidneys, the result can be fatal. It can also be found on medical equipment. Pseudomonas is well known to be animal and plant pathogen. It is also known to cause food spoilage. Over 50 different types of pseudomonas can infect humans, but most infections are caused by pseudomonas aeruginosa, which is known for its role as an opportunistic human pathogen. In the present study a total of 10 soil samples, 25 samples of urine and 25 samples of wound were collected from Health centre, Osmania University for the isolation of pseudomonas spp. All the samples were inoculated onto selective medium " Kings Media " for isolation of all pseudomonas spp. and Cetrimide agar for isolation of pseudomonas aeruginosa. A total of three pseudomonas aeruginosa isolates were selected for further studies taking one pseudomonas aeruginosa. from each of the source. All the three were identified by biochemical characteristics. For all the three strains Antibiotic susceptibility testing (AST) was carried out to determine the antibiotic sensitivity and resistant in plate culture conditions. Presence of plasmid DNA indicated the possibility of antibiotic resistance in pseudomonas spp. and also MIC (Minimum Inhibitory Concentration) for three strain of pseudomonas aeruginosa was detected.
Journal of clinical microbiology, 1999
The development of multidrug-resistant Pseudomonas aeruginosa in patients with cystic fibrosis (CF) is most likely a consequence of increasing life expectancy and more prolonged exposure to antibiotics. The optimal method for antibiotic susceptibility testing of CF strains, particularly mucoid P. aeruginosa strains, is unknown. Antimicrobial susceptibilities of 48 CF strains (25 mucoid) and 50 non-CF strains to 12 anti-Pseudomonas agents were tested by both agar dilution and commercially custom-prepared broth microdilution plates (PML Microbiologicals, Portland, Oreg.) in three laboratories simultaneously to determine if broth microdilution could substitute for agar dilution as the reference method in subsequent studies. Comparison of MICs generated by agar dilution and broth microdilution demonstrated correlation coefficients (r) exceeding 0.85 for all agents tested; correlation was excellent for aminoglycosides (r >/= 0.92) and very good for beta-lactam agents including agents ...