Homocysteine and lipid peroxidation markers in patients with coronary heart disease 29 Several potential mechanisms of homocysteine (original) (raw)
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Oxidative stress and homocysteine in coronary artery disease
Clinical chemistry, 2001
Oxidative stress is present in cardiovascular diseases (CVDs), and hyperhomocysteinemia, an independent risk factor for these diseases, may play a role by inducing production of oxygen free radicals. To evaluate the possible role of homocysteine (Hcy) in inducing oxidative stress in coronary artery disease (CAD), plasma Hcy was measured in 68 consecutive cardiovascular patients, and plasma malondialdehyde (MDA), both free and total (free + bound), was measured in 40 patients with CAD (18 with chronic stable angina and 22 with unstable angina). As controls, we tested 70 healthy volunteers. Hcy was measured by an immunoenzymatic method and MDA, an index of lipid peroxidation, by gas chromatography-mass spectrometry. Plasma Hcy concentrations were significantly higher in cardiovascular patients than in controls (10.2 vs 8.9 micromol/L; P <0.0002), with no significant difference between values in the stable and unstable angina subgroups. Similarly, total MDA was significantly higher ...
Al-Mustansiriyah Journal of Science
Homocysteine (Hcy) has been considered as an independent risk factor for coronary artery disease (CAD Oxidative stress and free radicals are known to have important roles in the development ofAngina Pectoris. Oxidative stress is present in cardiovascular diseases (CVDs), and hyperhomocysteinemia, an independent risk factor for these diseases, may play a role in inducing production of oxygen free radicals. The aim of this study was to determine a possible relationship between blood serum Hcy levels and lipid peroxidation in patients suffering from Angina Pectoris (AP).To evaluate the possible role of homocysteine (Hcy) in inducing oxidative stress in Angina Pectoris(AP), plasma homocysteine( Hcy), plasma malondialdehyde (MDA) and C reactive protein (CRP) were measured in 60 unstable Angina Pectoris patients, we tested 30 healthy volunteers. Hcy was measured by an enzymatic colorimetric method and MDA, an index of lipid peroxidation, by spectrophotometer. Serum Hcy levels were signifi...
2019
Published 15/08/2019 Homocysteine thought to be free indicators for the disease like coronary artery. Oxidative stress and free radicals plays important roles in the progressing of Angina Pectoris. Previously, scientists has proven that Oxidative stress exists in patients with cardiovascular diseases (CVDs), and hyperhomocysteinemia, Aiding in inducing production of oxygen free radicals thus determining level of oxidative stress, could help health professionals to predict these kind of diseases. The purpose of our work was to explore whether blood serum homocysteine Hcy levels in patients with Angina Pectoris (AP) has any impact on rate of their lipid peroxidation. To see the probability effect of homocysteine (Hcy) in the oxidative stress activations of those patients. To find levels of the plasma homocysteine( Hcy), plasma malondialdehyde (MDA) and Creactive protein (CRP) were measured in 60 unstable Angina Pectoris patients, also 30 healthy volunteers has been examined. Hcy was m...
Assessment of lipid per-oxidation and endothelial dysfunction in patients of coronary artery disease
IP innovative publication pvt. ltd, 2019
Abstract Aim: To find the difference in the levels of homocysteine and lipid-peroxidation in male and female patients of coronary artery disease. Materials and Methods: 71 subjects were included in this study over a period of 12 months out of which 12 female patient of CAD, 29 male patient of CAD and 30 were normal healthy subjects as controls. Estimation of plasma malondialdehyde (MDA) was done by colorimetric technique on RA 50 semi-automated chemistry analyzer. The homocysteine level in the plasma was estimated using the Hcy enzymatic assay on SYNCHRON CX5, Automated Chemistry Analyzer of Beckman Coulter Ltd. Results: We found significant increased levels of Hcy and MDA in male CAD patients than in female CAD patients. Conclusion: In female CAD patients, decreased levels of Hcy and MDA areindication of low oxidative stress that may be due to female sex hormones. Keywords: Coronary artery disease (CAD), Homocysteine (Hcy), Lipid-peroxidation, S-adenosylhomocysteine (SAH).
Enhanced In Vivo Lipid Peroxidation at Elevated Plasma Total Homocysteine Levels
Arteriosclerosis, Thrombosis, and Vascular Biology, 1999
An elevated plasma total homocysteine level (tHcy) is considered an independent risk factor for atherosclerosis. The mechanisms by which hyperhomocysteinemia induces atherosclerosis are only partially understood, but promotion of LDL oxidation and endothelial injury have been suggested. The purpose of this study was to test the hypothesis that a high plasma tHcy is associated in men with increased in vivo lipid peroxidation, as measured by plasma F 2 -isoprostane concentrations. We investigated this association in a subset of the participants in the Antioxidant Supplementation in Atherosclerosis Prevention (ASAP) study. Of 256 male participants, a subsample of 100 consecutive men was selected for F 2 -isoprostane assays. The mean tHcy was 11.0 mol/L, and the mean F 2 -isoprostanes was 29.6 ng/L. The simple correlation coefficient for association between tHcy and F 2 -isoprostane was 0.40 (PϽ0.001). In a linear regression model, the variables with the strongest associations with F 2 -isoprostane were tHcy (standardized coefficient 0.33, PϽ0.001), serum triglycerides (0.21, Pϭ0.042), carbohydrate-deficient transferrin (0.15, Pϭ0.132), and plasma lipid-standardized ␣-tocopherol (Ϫ0.11, Pϭ0.252) (R 2 ϭ0.24, PϽ0.001 for model). Plasma F 2 -isoprostane levels increased linearly across quintiles of tHcy (PϽ0.001). The unadjusted mean (95% confidence interval) F 2 -isoprostanes was 47.5% greater in the highest tHcy quintile (37.4, 31.1 to 43.6 ng/L) than in the lowest quintile (25.3, 21.3 to 29.3 ng/L). Adjustment for the strongest other determinants of F 2 -isoprostane reduced this difference to 28.2% (Pϭ0.010). Our present data suggest that elevated fasting plasma tHcy is associated with enhanced in vivo lipid peroxidation in men.
African Journal of Biotechnology, 2012
Oxidative stress has been implicated in coronary artery disease (CAD). Malondialdehyde (MDA) is lipid peroxidation end product. Bilirubin may act as an antioxidant that suppresses lipid oxidation. The role of MDA and antioxidant capacity and their inter-relationship in patients with and without CAD was investigated. Thirty-eight consecutive patients with angiographically diagnosed CAD were compared with 60 age, and sex-matched controls. The controls had completely normal coronary arteries in angiograms. Plasma MDA, serum bilirubin, total homocysteine and total antioxidant capacity (TAC) levels were measured. Risk factors of CAD were determined for all subjects using National Cholesterol Education Program (NCEP)-Adult Treatment Panel (ATP)-III criteria. Serum MDA and total homocysteine concentration were significantly higher, but TAC, total bilirubin and direct bilirubin levels were lower in CAD patients when compared to the controls. Age, and sex-adjusted plasma MDA levels had negative correlations with TAC (r = -0.30, p = 0.001) and total bilirubin (r = -0.30, p = 0.002) concentrations. In multivariate analysis by the multiple logistic regression method, serum MDA was significantly associated with CAD (OR = 1.15, 95% CI, 1.25 to 1.82; p < 0.0001)) after adjustment for lipid status parameters and traditional risk factors in this study population. Increased serum MDA concentration, as a biomarker of lipid peroxidation, low serum bilirubin and antioxidant capacity were observed in patients with angiographically defined CAD. The significant inverse correlation of the serum bilirubin and MDA levels demands further in-depth investigations to clarify the association between them in the development of CAD.
Lipid peroxidation and the levels of antioxidant enzymes in coronary artery disease
Indian Journal of Clinical Biochemistry, 2008
Coronary Artery Disease is the major cause of mortality and morbidity worldwide. Traditional risk factors account for only half of the morbidity and mortality from coronary artery disease. There is substantial evidence that oxidative stress plays the major role in the atherosclerotic process. The present study was undertaken to evaluate the level of lipid peroxidation (by measuring malondialdehyde) and antioxidant enzymes (ceruloplasmin, glutathione, superoxide dismutase) in coronary artery disease. Serum malondialdehyde levels and serum ceruloplasmin levels were significantly raised in all the subgroups of study group as compared to control group (p<0.001). Whole blood glutathione levels and hemolysate superoxide dismutase activity was significantly decreased in all the subgroups of study group as compared to control group (p<0.001). Above results suggests that the patients of coronary artery disease show increased oxidative stress and decreased levels of antioxidant enzymes. So it is recommended that the management protocol for coronary artery disease patients should include antioxidant supplementation along with simultaneous lowering of lipid peroxidation.
Relationship of plasma homocysteine with lipid profile parameters in ischemic heart disease.
The present study was undertaken to explore the relationship of plasma homocysteine with other biochemical parameters in ischemic heart disease. Plasma levels of total homocysteine was measured by HPLC -fluorescence detection with internal standard in 60 ischemic heart disease patients and were compared with 30 age matched normal healthy controls. The significant increase of plasma homocysteine was observed in both myocardial infarction and chronic stable ischemic heart disease patients when compared with the controls. The hyperhomocysteinemia appears be to due to increased body demand of vitamins such as folic acid, vitamin B 12 , B 6 , B 2 either alone or in combination to regulate normal homocysteine metabolism.
Oxidant and antioxidant status in coronary artery disease
Biomedical reports, 2018
Formation of atherosclerotic plaques is the major cause of coronary artery disease (CAD). Several lines of study have revealed the role of oxidative stress in CAD pathogenesis. In the present study the aim was to investigate the oxidative and antioxidative markers in CAD patients and a control population. The study sample comprised of acute coronary syndrome (ACS) patients, chronic CAD patients and healthy controls (n=30/group). Blood samples of patients and control subjects were collected to measure the concentrations of reduced glutathione (GSH), malondialdehyde (MDA) and the percentage of MDA release as well as the activity of erythrocyte glutathione peroxidase (GPx) and total antioxidant capacity (TAC) of plasma. All parameters were measured by spectrophotometric methods. Additionally, oxidant/antioxidant status was compared between CAD patients with single, double or triple-vessel stenosis and in comparison with controls. The results indicated a significant increase in MDA leve...
Diagnostic Pathology, 2013
Background: An imbalance between pro-oxidants and antioxidant systems has been suggested to be implicated in the physiopathology of acute myocardial infarction (AMI). We aimed to evaluate the antioxidant capacity in Tunisian patients and to assess the possible relationship between erythrocyte catalase enzyme activity and hyperhomocysteinaemia. Methods: 108 patients with AMI and 81 healthy subjects were enrolled in this study. Catalase erythrocyte enzyme activity was determined spectrophotometrically whereas "total antioxidant status" (TAS) concentration was measured by a commercially available method. Serum total homocysteine (tHcy) level was determined by a fluorescence polarization immunoassay (FPIA). Lipid peroxidation was measured with a fluorimetric method as "thiobarbituric acid reactive substances" (TBARS). Results: Compared with healthy subjects, patients with AMI had significantly lower catalase activity (P<0.001), TAS concentrations (P<0.001), and significantly higher serum tHcy (P<0.001) and TBARS levels (P<0.001). Erythrocyte catalase enzyme activity was negatively correlated with serum tHcy and TBARS while serum tHcy and TBARS were in positive correlation. Furthermore, the unbalance between pro-oxidants and antioxidants seems to be more aggravated in patients with Q wave AMI compared to patients with non-Q wave AMI. Conclusion: Our results suggest the involvement of hyperhomocysteinaemia in the drop of erythrocyte catalase activity related to myocardial ischemia reperfusion. Hyperhomocysteinaemia may increase the myocardial wall dysfunction under ischemia reperfusion by excessive production of reactive oxygen species which is made evident by increased lipid peroxidation.