Potential smear interference with self-etch hybridization studied by TEM (original) (raw)

Abstract

Objectives: To evaluate whether hybridization of enamel/dentin by self-etch adhesives may be affected by smear. Methods: Two 2-step self-etch adhesives, the recently marketed Optibond XTR (O-XTR; Kerr) and Clearfil SE Bond (C-SE; Kuraray), were bonded strictly according to the manufacturer's instructions to either (1) bur-cut (100-m grit) enamel/dentin, (2) 600-grit SiC-paper ground enamel/dentin, or (3) smear-free un-cut enamel and fractured dentin (non-carious human third molars). The 3-step etch-and-rinse adhesive Optibond FL (O-FL, Kerr) served as control. After 1-day storage in water (37C), non-demineralized/demineralized 70-90 nm sections were prepared following common TEM-specimen processing, and eventually examined by TEM (JEM-1200EX II, Jeol). Results: At enamel, a tight bond based on only superficial interaction was observed for both O-XTR and C-SE. Especially at uncut enamel, hydroxyapatite rods appeared hardly dissolved (in contrast to phosphoric-acid etched enamel i...

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