Gibberellic acid and kinetin partially reverse the effect of water stress on germination and seedling growth in chickpea (original) (raw)
Related papers
Plant Growth Regulation, 2000
Enhanced amylase activity was observed during a 7-day-growth period in the cotyledons of PEG imposed water stressed chickpea seedlings grown in the presence of GA3 and kinetin, when compared with stressed seedlings. During the first 5 days of seedling growth, the seedlings growing under water deficit conditions as well as those growing in the presence of PGRs had a higher amylase activity in shoots than that of control seedlings. Neither GA3 nor kinetin increased the amylase activity of roots whereas IAA reduced root amylase activity. Activity of acid and alkaline invertases was maximum in shoots and at a minimum in cotyledons. Compared with alkaline invertase, acid invertase activity was higher in all the tissues. The reduced acid and alkaline invertase activities in shoots of stressed seedlings were enhanced by GA3 and kinetin. Roots of stressed seedlings had higher alkaline invertase activity and GA3 and IAA helped in bringing the level near to those in the controls. GA3 and kinetin increased the sucrose synthase (SS) and sucrose phosphate synthase (SPS) activities in cotyledons of stressed seedlings, whereas they brought the elevated level of SPS of stressed roots to near normal level. The higher level of reducing sugars in the shoots of GA3 and kinetin treated stressed seedlings could be due to the high acid invertase activity observed in the shoots, and the high level of bound fructose in the cotyledons of stressed seedlings could be due to the high activity of SPS in this tissue.
Plant Growth Regulation, 1998
The percentage germination of chickpea seeds (Cicer arietinum L.cv. PBG-1) gradually decreased with increasing concentration of NaCl in the growth medium and was completely inhibited with 200 mM NaCl. In the presence of 75 mM NaCl, only 51% of the seeds germinated. Gibberellic acid (GA3) and kinetin at 6 µM concentration induced the maximum increase in % germination and seedling growth under salt stress. However, IAA further inhibited both the germination and growth of stressed seedlings. The reduction in amylase activity in cotyledons of stressed seedlings was partially reversed with GA3 and kinetin whereas IAA did not show any positive effect. GA3 was more effective than kinetin in enhancing the reduced germination and seedling growth of chickpea seeds along with amylase activity in cotyledons under NaCl induced saline conditions. The reduced uptake of radiolabelled 14C sucrose by cotyledons and its reduced distribution in the shoots and roots of stressed seedlings was increased with addition of GA3 in the medium. Cotyledonary amylase was separated into amylase 1 and amylase 2 by sephadex G 150 column chromatography. The reduced activities of both amylase 1 and amylase 2 in cotyledons under salt stress was returned to near normal levels with GA3 and there was also an increase in starch utilization, resulting in its lower concentration in cotyledons of GA3-supplemented stressed cotyledons.
2016
Effects of different temperatures (10˚C, 15˚C, 20˚C, 25˚C and 35˚C) and various water potentials (0, −0.25, −0.5, −0.75, −1.0, −1.25, −1.5 MPa) on seed germination and early seedling development of two contrasting chickpea (Cicer arietinum L.) cultivars were studied. Different temperatures were applied in an incubator by adjusting the device according to the specific treatments. Water potential treatments were done by using different concentrations of polyethylene glycol (PEG), 8000 (molecular weight). The results showed significant differences between the two cultivars in all characters studied. The cultivar Arman exhibited the higher germination percentage and rate than the cultivar Pirooz. Water stress caused significant reductions in germination parameters at unfavorable temperatures (10˚C, 30˚C, and 35˚C). Seeds of both cultivars, which are subjected to high osmotic potentials, had high recovery percentages at the optimum temperature (25˚C). In conclusion, the cultivar Arman seems to be more tolerant to water stress at suboptimal and super optimal temperatures compared with Pirooz.
Effects of different temperatures (10,15, 20, 25 and 35°C) and various water potentials (0, -0.25, -0.5, -0.75, -1.0, -1.25, -1.5 MPa) on seed germination and early seedling development of two contrasting chickpea (Cicer arietinum L.) cultivars were studied. Different temperatures were applied in an incubator by adjusting the device according to the specific treatments. Water potential treatments were done by using different concentra-tions of polyethylene glycol (PEG) , 8000 (molecular weight). The results showed significant differences between the two cultivars in all characters studied. The cultivar Arman exhibited the higher germination percentage and rate than the cultivar Pirooz. Water stress caused significant reductions in germination parameters at unfavorable temperatures (10, 30, and 35 oC). Seeds of both cultivars, which are subjected to high osmotic potentials, had high recovery percentages at the optimum temperature (25 oC). In conclusion, the cultivar Arman seems to be more tolerant to water stress at suboptimal and super optimal temperatures compared with Pirooz.
Effect of Different Solutions on Seed Germination and Physiological Changes in Cicer arietinum
International Journal of Scientific Research in Science and Technology, 2021
The chickpea is rich source of vitamins, fibers and minerals; it provides variety of health benefits like aiding weight management, improving digestion and lessens the risk of several diseases. The chickpea is high in protein and it is the best way to replace meat in vegetarian and vegan diets. For seed germination of chick pea generally used growth hormones like gibberellic acid (GA), IAA, IBA, salicylic acid and etc, PEG (polyethylene glycol) and control is used for comparison. In present work, the seeds of chickpea (Cicer arietinum) are treated with hormones like gibberellic acid salicylic acid solutions, PEG solution and as stress there is salt stress (NaCl) and citric acid (CA) solutions to see the seeds germination under this circumstances, and seeds also germinated in control (distilled water) condition for comparison in petridish on whatman paper for 10 days after that the germinated seeds (PEG, gibberellic acid and distilled water) transferred to the mud pot with the soil a...
Legume Research- An International Journal, Volume 46 Issue 12: 1583-1589 (December ), 2023
Background: Chickpea (Cicer arietinum L.) is one of the most important pulse crops in the world. In Tunisia, the chickpea is vulnerable to fluctuation in production in the last few decades. However, phytohormones are known to play crucial roles in regulating different development processes in plants. Methods: This research was conducted in Petri dish and pot experiments in order to determine the effect of gibberellic acid (GA3) and indole butyric acid (IBA) phytohormones on some growth parameters of chickpea. In this study, GA3 at concentrations of 0, 0.05, 0.1, 0.15 and 0.2 g/l and IBA at concentrations of 0, 10-5 , 10-4 , 10-3 and 10-2 g/l were applied at different stages of chickpea development. Result: Results of Petri dishes trial revealed that IBA (10-4 g/l) and GA3 (0.2 g/l) hormones improved significantly the shoot and root length of chickpea plant. Results of pots pointed out that the chickpea achieves the highest plant height when IBA (10-4 or 10-3 g/l) and GA3 (0.2 g/l) were used at pre-flowering stage. The IBA and GA3 application with concentrations of 10-3 g/l and 0.1 g/l, respectively allowed the highest nodule number at post-flowering stage. The parameters number of branches, fresh and dry weight of the aerial part and leaves number were improved in chickpea plants treated with IBA and GA3 at pre-flowering and post-flowering, respectively. Similarly, the number of flowers was promoted by the two hormones. The weight and the number of seeds were significantly enhanced by all IBA treatments in plants. An increase in weight and the number of seeds was observed at post-flowering phase of GA3 treatment. Thus, these results identified the beneficial effect of tested phytohormones in chickpea growth.
Response of chickpeato gibberellic acid (GA3) regarding morpho-physiology and yield was investigated in this experiment. The experiment consisted of two factors: a) two growing conditions viz.; control and foliar application of GA3 and b) four chickpea varieties viz.; Deshi, BARI Chola-3, BARI Chola-5 and BARI Chola-9. The experiment was laid out in Randomized Complete Block Design with three replications. Plant height, leaf dry weight and shoot and root dry weight plant-1 at different days after sowing maintained a typical sigmoid pattern in all chickpea varieties under both control and GA3 applied condition. The percentage of increasing in maximum plant height, leaf dry weight plant-1 , shoot dry weight plant-1 and root dry weight plant-1 were 6.21%, 9.59%, 12.00% and 9.05% in Deshi variety; 4.54%, 3.06%, 4.89% and 4.76% in BARI Chola-3; 5.21%, 9.28%, 8.25% and 10.07% in BARI Chola-5 and 8.31%, 13.44%, 9.80% and 9.58% in BARI Chola-9, respectively under foliar application of GA3. The combined effect of growing condition and chickpea variety significantly influenced number of flowers plant-1 number of pods plant-1 , 100-seed weight, biological yield and seed yield. BARI Chola-9 produced the maximum seed yield under both control and foliar application of GA3 (2.45 and 2.71 t h-1 , respectively) on the contrary BARI Chola-3 produced the minimum seed yield (1.86 and 1.97 t h-1 , respectively). Foliar application of GA3 resulted increasing in all traits of chickpea varieties but the magnitude of increasing was not similar to all varieties. BARI Chola-9 performed better compared to other three varieties in relation to foliar application of gibberellic acid (GA3).
Plant Growth Regulation, 2002
Seven-day-old seedlings obtained from seeds primed with mannitol (4%)and water showed three to four fold more growth with respect to root and shootlength in comparison with seedlings obtained from non-primed seeds. Seedlingswere grown under water deficit stress conditions created by 15% polyethyleneglycol (PEG) 6000 in the medium. Priming of chickpea seeds with NaCl and PEGwasnot effective in increasing seedling growth under these water deficit stressconditions. The activities of amylase, invertases (acid and alkaline), sucrosesynthase (SS) and sucrose phosphate synthase (SPS) were higher in shoots ofprimed seedlings. An increase in the activities of SS, and both the acid andalkaline invertases was also observed in roots of primed seedlings. The twofoldincrease in specific activity of sucrose phosphate synthase was observed incotyledons of primed seedlings. The higher amylase activity in shoots of primedseedlings enhanced the rapid hydrolysis of transitory starch of the shootleading to more availability of glucose for shoot growth and this was confirmedby the low level of starch in shoots of primed seedlings.
Effect of growth regulators on germination and vigour of Chickpea (Cicer arietinum L.) seed
Journal of Pharmacognosy and Phytochemistry, 2017
A study was conducted during 2016-17 to evaluate the effects of organic and inorganic growth regulators on germination and vigour of Chickpea (Cicer arietinum L.) seed at Post Graduate laboratory, Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad (U.P.). The experiment consist of three concentration of GA3 (10, 20 and 30 ppm), three concentration of IAA (10, 20 and 30 ppm), three concentration of lemon juice (2, 4 and 6%), and three concentration of Panchgavya (2, 4 and 6%). The experiment was laid out in completely randomized block design with four replications. T8 (GA3 20ppm) proved significantly superior in germination percent, germination index, shoot length, root length, seedling length, fresh weight of seedling, dry weight of seedling, seed vigour index-I, and seed vigour index-II.
2018
Seed priming is a simple pre-germinating strategy to improve seed performance and alleviate the negative effects associated with stress exposure. Seed priming technique was used in different plants to improve seed germination and tolerance on stress exposure. The aim of this study was to evaluate the effects of H O on seed germination and vegetative growth of different plants. Overall the tolerance index was 2 2 improved under salt treatment when the seeds were pretreated with H O 2 2.