Detection and characterization of DHA-1 beta-lactamase in Klebsiella spp. causing urinary tract infections in Bosnia and Herzegovina (original) (raw)

2014

Abstract

Objectives Klebsiella spp. is a common cause of urinary tract infections (UTIs) and it is important nosocomial pathogen. There is currently little information about a prevalence of AmpC beta-lactamases in these strains. The aim of the study was to investigate a prevalence of plasmid –mediated AmpC (pAmpC) beta-lactamases in Klebsiella spp. causing UTIs in Bosnia and Herzegovina (B&H), during December 2009-May 2010. Methods Minimum inihibitory concentrations (MICs) of various antibiotics were determined by broth microdilution method according to CLSI guidelines. Double-disk synergy test and combined disk test with phenylboronic acid were used to detect ESBLs and pAmpC beta-lactamases respectively. BlaESBL, and blaAmpC genes were detected by PCR. Genetic relatedness of the strains was tested by pulsed-field gel electrophoresis (PFGE). Results ESBL. were found in 28 Klebsiella spp (22 K. pneumoniae and six K. oxytoca). Genes encoding pAmpC beta-lactamases were detected in two (7.1%) is...

Amir Ibrahimagić hasn't uploaded this paper.

Let Amir know you want this paper to be uploaded.

Ask for this paper to be uploaded.