Evaluation of Effect of Nishamalaki on STZ and HFHF Diet Induced Diabetic Neuropathy in Wistar Rats (original) (raw)

Diabetes Mellitus (DM) is a metabolic disorder; alarmingly increase in the incidence observed globally. DM considered as a major threat to human health in developing and developed countries due to its complications. In spite of control of DM, complications may develop over due course of disease. Neuropathy is the most common chronic complication of DM affecting more than 50% of the patients [1]. The duration of disease and level of hyperglycaemia are important determinants of microvascular complications of diabetes [2]. Pain due to diabetic neuropathy affects most often lower extremities above the knees and upper extremities [3]. Abnormal excitement of immaturely regenerated nerve fibers causes spontaneous pain, numbness and paresthesia [4]. Pain occurs even after the exposure to mild pain stimuli i.e., hyperalgesia and allodynia. Diabetic neuropathy shows significant impact on Quality Of Life (QOL) and costs of management [5]. Apart from tight glycaemic control, no other evidence-based treatments are known to prevent neuropathy [6]. The exact pathology of diabetic neuropathy is not known, but it involves oxidative stress, advanced glycation end products, polyol pathway flux activation contribute to microvascular disease and nerve dysfunction [7]. Current treatment based on the use of desipramine, amitriptyline, capsaicin, tramadol, gabapentin, bupropion, and venlafaxine etc., [8]. But these drugs provides only symptomatic relief and has no effect on Blood Sugar Level (BSL) and progression of disease. Nishamalaki (Na) is an Ayurvedic formulation prepared from Amla (Emblica officinalis) and Turmeric (Curcuma longa) with the traditional method as per the ancient literature [9]. Traditional use mentioned its utility to control DM in initial phase and continued with addition of other agents for maintenance [10,11]. Individual agents in NA show hypoglycaemic, anti-oxidant and neuro-protective effect [12,13]. No studies are available for effect of combined formulation using modern medicinal parameters for neuropathy. Present study was planned to assess the efficacy of NA in diabetic neuropathy. MatERIals and MEthods Wistar rats of either sex having average weight of 150-200g were used for the study. Housing was done in accordance with Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) guidelines in standard cages. The animals were given food and water ad libitum and were exposed to12 hours light and dark cycle. Study started after obtaining the approval (IAEC:BVDUMC/88/2014-15

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