Phytochemical investigation of bioactive Emodin and quercetin in Cassia fistula and Cassia tora plant parts by HPTLC (original) (raw)
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Simultaneous estimation of aloe emodin and emodin from Rheum emodi, Cassia alata and Aloes by HPTLC
Indian Journal of Pharmaceutical Sciences, 2015
Narayanan and Jadhav: Estimation of Aloe Emodin and Emodin by HPTLC A simple, precise, specific, accurate high performance thin layer chromatography method was developed for simultaneous estimation of aloe emodin and emodin from medicinal plants like Rheum emodi (Rhubarb), Barbados aloes (dried juice of Aloe barbadensis leaf) and Cassia alata (Candle bush). Thin layer chromatographic aluminum plates pre-coated with silica gel 60 F 254 was used as the stationary phase for chromatographic separation of the drugs. Toluene:ethyl acetate:formic acid (10:2:1 v/v/v) was selected as mobile phase and analysis was carried out in absorbance mode at iso-absorptive wavelength of 263 nm. This method shows good resolution for both drugs with retention factor 0.37±0.03 and 0.55±0.03 for aloe emodin and emodin, respectively. The regression analysis data indicated good linear relationship for the calibration plots for aloe emodin and emodin in the range of 300-800 ng/ spot and 150-400 ng/spot and regression coefficient was 0.9993 and 0.9994, respectively. Validation of the method was performed according to International Conference on Harmonisation guidelines for following parameters: Accuracy, precision, limit of detection, linearity, limit of quantification, robustness and specificity. In conclusion, the developed method was found to be rapid, simple, reliable and specific for the identification and quantitation of these anthraquinones in medicinal plants and marketed formulations.
Journal of Pharmacognosy and Phytochemistry, 2019
Cassia auriculata (CA) Linn (vernacular name: Avartaki,) is a traditional ayurvedic medicine used in the treatment of Diabetes mellitus. The present study was aimed for extraction, isolation, method development, optimization and validation of RP-UFLC method for bioactive flavonoids such as quercetin-3-O-rutinoside & quercetin from Cassia auriculata. Reverse phase Ultra Fast Liquid Chromatography method was developed with help of UV detector for isolated compounds. Method validation was performed as per international conferenece of harmonization guidelines for system suitability, linearity, accurcay, precision, specificity, robustness, limit of detection and limit of quantification of quercetin and quercetin-3-O-rutinoside. Intra-day and inter-day precisions for quercetin 3-O-rutinoside and quercetin was found precise as ≤ 2.0% &< 4.0% in intraday exercise & ≤2.0% &< 3.0% in inter day exercise. The concentration found in Cassia auriculata are 0.05 μg/mL of quercetin and 0.16 μg...
Phytochemical evaluation and HPTLC fingerprint profile of Cassia fistula
2014
The present study was aimed to develop the high performance thin layer chromatography (HPTLC) finger print profile of methanol and ethyl acetate extracts of leaves of Cassia fistula. Chromatographic technique was used for separation of components from different extracts of leaves. This study was planned to develop a HPTLC fingerprint profile of extracts in different solvents such as petroleum ether, toluene, ethyl acetate, chloroform, acetone and formic acid. A HPTLC method for the separation of the active constituents in extracts has been developed and TLC of these extracts on silica gel pre-coated aluminum plates of Merck by automatic TLC applicator and using solvent system toluene: ethyl acetate: formic acid::5:4:1 was performed. HPTLC profiling of the extract confirm about the presence of various phytochemicals. HPTLC finger print scanned at 400 nm for methanol and ethyl acetate leaf extracts revealed 15 and 16 peaks with Rf values in the range of 0.06 to 0.99 and 0.02 to 0.98 r...
Journal of Liquid Chromatography & Related Technologies, 2001
of Maced on ia , Institute of Pharmac ogno sy, Faculty of Pharmacy, Vod nj an ska 17, 1000 Skopje, Repub lic of Maced on ia ABSTRACT A new and ra pid procedure for sc reening of flavonols (rnyrice tin, querce tin and kaernpferol) and fo r determ ination o f quer cetin by RP-I-IP LC w ith UV-di ode array detecti on in 16 medi c inal plants is presen ted . Scree ning of the ex trac ts sh owed that q uerce tin is the most abundan t flavon ol , es pecia lly in Hyp erici herba , Uvae ursifolium and Pruni sp inosaejlos . Kaemp ferol was the most ab unda nt in Rvbi niae pse udoaca ciae flos an d Pruni spin osaeflos, whe reas myricetin was identified onl y in Betulae folium.
Pharmacognosy Journal, 2018
Background: Cassia auriculata (Avaram) is an important medicinal plant in India Improved awareness in medicinal flowers has led to an increased need for efficient extraction methods and screening of flavonoid derivatives. Objective: To standardization of extraction solvent system and Characterization of flavonoids through positive and negative electrospray ionization mode using LC-MS/MS from Cassia auriculata extract. Materials and Methods: The different solvents like Methanol, Water, Acetonitrile, Ethyl Acetate, Ethanol, Chloroform, Hexane, Acetone Diethyl ether used frot he identification of flavonoids (Gallic acid, Theanine, Theobromine, Theophylline, Caffeic acid, Caffeine, Ferulic acid, Theacrine, Catechin, Quercetin, EpiGallo Catachin, catechin gallate, Epicatachin gallate and Quercetin hexoside. Results: Based on the peak area percentage the extraction solvent was standardized. The percentage of relative & absolute intensity of screened flavonoids was observed using LC-MS in positive and negative electrospray ionization. The results show that the methanol extract has more percentage of peak area, relative intensity and absolute intensity. The MS results showed that the negative ionoization has more intensity values of flavonoids and the signal-to-noise ratio was high in negative ionization mode compare to positive mode. Conclusion: Based on the results the methanol is the suitable extraction solvetnt and negative ionization mode of ESI-LC-MS/MS was appropriate for the screening of flavonoids on Cassia auriculata flower extracts.
Journal of Separation Science, 2007
A simple, sensitive, selective, precise, and robust high-performance TLC (HPTLC) method was developed and validated for determination of flavonoids in herbal extracts Bauhinia variegata, Bacopa monnieri, Centella asiatica, Ginkgo biloba, Lonicera japonica, Rosa bourboniana, Rosa brunonii, and Rosa damascena. The HPTLC of flavonoids was performed on RP-18 F254 TLC plates with dual run, water (5% formic acid)/methanol (70:30) and water (5% formic acid)/methanol (50:50) as mobile phases. Densitometric determination of flavonoids was performed at λ = 280 nm in reflectance/absorbance mode. The linear regression analysis data for the calibration plots showed a good linear relationship with r2 = 0.998 ± 0.0003 in the concentration range of 150–800 ng/spot for apigenin and rutin and 200–1000 ng/spot for quercetin, luteolin, and quercitrin with respect to peak area. The average recovery for apigenin, quercetin, rutin, luteolin, and quercitrin was 97–99.8% indicating the excellent reproducibility. Statistical analysis of the data showed that the method is reproducible and selective for determination of flavonoids.
Mass Spectrometry Letters, 2020
A systematic isolation and characterization study for Cassia auriculata (CA) seeds resulted in identifying antidiabetic compounds 1,3,8-trihydroxyanthraquinone and quercetin, quercetin-3-O-rutinoside, gallic acid, caffeic acid, ferulic acid, and ellagic acid. The ultra-high-performance liquid chromatography based triple quadrupole mass spectrometry methodology was developed and validated for simultaneous identification and confirmation of these compounds from CA seeds. Multiple reaction monitoring (MRM) based quantification method was developed with MRM optimizer software for MS 1 and MS 2 mass analysis. The method was optimized on precursor ions and product ions with the ion ratio of each compound. The calibration curves of seven bioactive analytes showed excellent linearity (r 2 ≥ 0.99). The quantitation results found precise (RSD, < 10 %) with good recoveries (84.58 to 101.42%). The matrix effect and extraction recoveries were found within the range (91.66 to 102.11%) for the CA seeds. This is the first MS/MS-based methodology applied to quantifying seven antidiabetic compounds in CA seeds and its extract for quality control purposes.
International Journal of Experimental Research and Review, 2023
The large and untapped resources of chemical compounds with significant medical potential found in medicinal plant species make them valuable as sources for biomedicine. Traditional medical systems like Ayurveda and Chinese Traditional Medicine are just two of the many that use the essential medicinal plant Cassia fistula L. It is a medium-sized tree that loses its leaves in the autumn. Its name is from its beautiful yellow flowers and long, rod-shaped seeds with pulp inside. The plant's roots were extracted using petroleum ether, chloroform, alcohol, and water in that order, and the products were looked at for preliminary phytochemical screening and antioxidant activity. Analysis of phytochemicals like alkaloids, flavonoids, carbohydrates, glycosides, protein, amino acids, saponins, and triterpenoids showed that most elements were present. Furthermore, this work concentrated on isolating and purifying aloe-emodin and Physcionusing column chromatography and then determining their purity using HPTLC. Different extraction solvents were tested, and ethanolic produced the highest yield of both. Toluene: Ethyl acetate: Methanol at 5:4:1 (v/v) showed superior resolution of Rf values at 0.7 and 0.9 as aloe-emodin and Physcion, respectively. Different solvents with different polarities were tried before using TLC to separate aloe-emodin and Physcion. The Toluene-ethyl acetate-formic acid ratio was increased when the silica gel column chromatography polarity was applied to the alcohol extract.
Quantitative Estimation of Quercetin in Mimusops elengi L.(Bakul) Leaves by HPTLC
scholarsresearchlibrary.com
Mimusops elengi L. is a medicinally valuable herb in the Ayurvedic and traditional systems of medicine. Various activities have been reported in almost all parts of Mimusops elengi, some of which includes diuretic activity, antidiabetic, antibacterial etc. Quercetin, one of the most important flavanoid is active against various cardio vascular diseases, cancer, tuberculosis, neurological diseases, cataract etc. In the present study High Performance Thin Layer Chromatography has been developed for detection and quantification of quercetin in Mimusops elengi leaves. Increasing serial dilutions of reference standard quercetin (200 to 1000 µg/mL) were scanned at 366 nm to detect and quantify the concentrations of quercetin in the test sample.The estimated values obtained from the same was 19.191mg/gm quercetin in powdered leaf sample.The method provided a rapid and easy approach for detection and the quantitation of the bio-marker quercetin. The authors also aim to validate the present method in terms of ruggedness and accuracy and undertake the isolation of quercetin from the said plant.
Chemical Data Collections, 2019
Quercetin, a member of a large family of polyphenolic compounds, has received remarkable attention due to its overwhelming presence in nutritional supplements. It is believed to have protective effects against several degenerative diseases by preventing lipid peroxidation. In the current study, the estimation of quercetin in methanol extracts of four medicinal plants including Amaranthus cruentus, Berberis calliobotrys, Solanum muricatum and Ribes himalense was made by our developed fluorescence spectroscopic method coupled with PLSR. The results obtained indicated that methanol extract of R. himalense exhibited higher quercetin content (3.83 ± 0.01%) followed by B. calliobotrys (1.91 ± 0.01%), A. eruentus (1.83 ± 0.02%) and S. muricatum (0.87 ± 0.05%). These results were also cross validated with UV-visible spectroscopy and found to be in close proximity to florescence emission method.