Diterpenoids from Vellozia flavicans (original) (raw)

EXPERIMENTAL The air dried aerial parts of M. zeylanica (400 g, collected in Sri Lanka, voucher No. NKBA 34) was extracted with Et&MeOH-petrol, 1: 1: 1, and worked-up as reported previously [9]. By CC (SiOl) and TLC (SiOz, PF 254) 40 mg /?farnesenc, 20 mg squakne, 25 mg dammadienyl acetate, 20 mg caryophyllen-1,10_epoxidt, 20 mg 1 and 5 mg S$dihydroxy-6,7,8,~-tetmmethoxyflavone were obtained and identified by ~rn~r~ng the400 MHz 'H NMR spectra with those ofauthentic material. The polar CC fraction (Et+MeOH, 9: 1) gave by HPLC (RP 8, MeOH-HsO, 7:3, ca 100 bar) 2 mg 3 (R, 3.5 min) and 10 mg 2 (R, 4.0 min). IO, 11 -Dihydromicroglossic acid (2). Colourlcss oil; IR v$'$cm -I: 35OO-2700,172O (COIH), 1740 (C02R); MS m/z (rel. int.): 364.225. [Ml+ (2) (cak. for CIIHsIO,: 364.225). 346 [M-i&O]-(14),332[M-MeOH-J'(5).324[346-MeOH]' (7). 108 (51). 95 (74). 94 (72), 83 (98), 82 (98), 81 (68), 69 (100). Microg/ossic aeid (3). Colourless oil; MS m/r (rel. int.): 362.209 [M] + (6) (cak. for CIt HJ005: 362.209). 330 [M -MeOH] ' (5). 284 [330-HCOzH]+ (IO), 256 [284-CO]' (12), 121 (60). 69 (100).