Analysis of Betalains (original) (raw)

2018

Abstract

Betalains are frequently present in composite mixtures. They quickly decompose in the separation stages and refining that makes separation of higher amounts of pigments difficult. Identification and structural elucidation of betalain molecules comprise of comparison of electrophoretic, chromatographic, and spectroscopic properties and involve direct comparison of these properties with reliable criterions before and after well-organized hydrolysis. The sensitivity of betalains to acid hydrolysis makes them different from anthocyanins, and their color changes with pH changes. In electrophoretic and chromatographic characteristics, both can be distinguished by simple color tests. Since both types of pigments occur exclusively, color tests are usually performed by using crude plant extracts. HPLC is the top-benched method in both quantifiable and semi-preparative works on betalains. The current chapter highlights the history of analyses and the most suitable contemporary methods and tec...

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