The G protein of vesicular stomatitis virus has free access into and egress from the smooth endoplasmic reticulum of UT-1 cells (original) (raw)

We have investigated the role of the smooth The ability of this mutant G protein to resume its intracellular transport at 32°C has made it especially useful in the study of intracellular transport (5, 8, 47). We used this feature of the ts045 G protein to examine the kinetics of its export from the SER. Again we infected UT-I cells that had extensive SER with ts045 and incubated them at 40°C. After 2 h, we added cyclobeximide and shifted the cells to the permissive temperature for 0, 15, or 30 min before fixation