Micropropagation in Moringa oleifera Lam for high-throughput Multiplication (original) (raw)
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Some important aspects in Moringa oleifera Lam. micropropagation
Acta agriculturae Slovenica
Type and source of explant as well as the type of cytokinin were important factors for successful moringa micropropagation. Explants obtained from in vitro grown plant materials were better than others obtained from soil growing seedlings. In addition, nodal segments were better than shoot cuttings in terms of number of shoots/ explants, frequency of shoot formation and number of nodes/shoot. While callus formation on the base of nodal segment on BAP containing media were higher than those of KIN, especially under the influence of high concentration as an aspect of vitrification, BAP was better than KIN in moringa multiplication. Low nutrient medium (half strength MS) supplemented with 0.5 mg l-1 IAA was essential for successful root formation. The suitable conditions for moringa micropropagation on full strength MS or SH may exert low stress and low need to raise the expression of SOD and POX. On the other side, stress due to over increase of chemical components of double MS medium...
International Journal of Advanced Research (IJAR), 2019
Moringaoleifera(G.H.No.:95436,Dr.MuneebaKhan,CentreforPlantConservation,University of Karachi) known as the miracle tree, is a plant of great importance. The plant is well known for its diverse medicinal and nutritional properties. The objective of the research is focused on tissue culture of Moringaoleifera through callus induction, direct multiplication as well as from seed culture.Thestudyisalsofocusedonoptimizationofsterilizationprotocolandmediaforthetissue culture processes. As expected, Moringaoleifera gave promising results using MS Media supplementedwithdifferentconcentrationsofPlantGrowthRegulatorssuchasIAA,NAA,BAP, Kinetin and 2,4-D. Seed initiation gave optimum results on MS Basal media. Direct multiplication of the plant was observed with optimum results on MS media supplemented with 0.1 mgL-1BAP. For callus induction, effects of Plant Growth Regulator and photoperiod were observed. In Dark conditions, maximum growth was seen on MS media supplemented with 2, 4-D (2 mgL-1), and that in light conditions, MS media with 2,4-D (2 mgL-1and 3 mgL-1) gave almost similar results. Initially, seed coats were removed inside the Laminar Flow Hood but this was affecting the sterilization negatively. Seed sterilization was then modified by removing seed coats and sterilizing them outside the Laminar Flow Hood. The seeds were then transferred to the LFH for furthersterilization,andthenculturedonthe growthmedia.Forthesterilizationoftheexplantfor directmultiplicationandcallusinduction,firstlyEthanolwasusedwhichcausedbleachingeffect. Later, Ethanol was removed from the protocol, reducing the bleaching effect to zero, and sterilization was maximized.
In vitro regeneration of Moringa oleifera Lam.: A medicinal tree of family Moringaceae
Indian Journal of Genetics and Plant Breeding (The)
A protocol is developed for somatic embryogenesis and organogenesis from nodal segments and shoot-apices of Moringa oleifera Lam. Callusing and multiple shoots induction occurred in nodal segments (0.5-1.0 cm) after 2 weeks of culture on Woody Plant Medium, 1981 (WPM) containing N6-benzyladenine (BA), alone or in combinations with various concentrations of á-naphthalene acetic acid (NAA). The concentration of BA at 4.44 μM was found optimum for multiple shoots formation, resulting in 3.22 ± 0.17 shoots per explants. WPM was enriched by adding certain additives to minimize yellowing and shedding of leaves in proliferated shoots. Maximum roots induction (86.6%) was observed in enriched WPM containing 2.46 μM IBA. Somatic embryos were developed from the calli obtained from nodal segments on enriched WPM supplemented with IBA and BA. Histological studies were carried out for studying the pattern of somatic embryogenesis. Multiple shoots formed from shoot apices (1.0-1.5 cm) cultured on ...
Enhanced micropropagation protocol of Morinda citrifolia L. through nodal explants
Journal of Applied Research on Medicinal and Aromatic Plants, 2015
We report an improved in vitro propagation protocol for Morinda citrifolia L. in the present communication using nodal segments as explants from a 4 year old plant. Murashige and Skoog (MS) medium was used to inoculate the sterilized explants. Under laboratory conditions, 4.6 ± 0.48 shoots were regenerated from the nodal meristem of an explant on the combination of MS medium fortified with 4.0 mg/L 6benzylaminopurine (BAP). Semi-solid (with agar) and liquid MS medium was used for the multiplication of shoots in vitro. Combined effect of BAP and kinetin in shoot multiplication was reported favorable in semi-solid (10.6 ± 0.17 shoots per vessel) and liquid MS medium (11.4 ± 0.47 shoots per vessel). The shoots were rooted in vitro on half-strength agar-gelled MS medium supplemented with 1.0 mg/L indole-3 butyric acid (IBA) and 44.30 roots per shoot were observed from the cut ends of the shoots. All the shoots were also rooted by ex vitro method and maximum 36.12 roots per shoot were induced by treating the shoots with 300 mg/L IBA for 5 min. The in vitro rooted and ex vitro treated plantlets were transferred to the soilrite containing paper cups for hardening in the greenhouse. The plantlets were shifted to nursery bags after 4 weeks. Finally the hardened plantlets were planted in the field with 100% rate of survival under natural conditions. The ex vitro rooting was reported more advantageous than in vitro rooting in terms of cost, time and percent survival of plantlets.
Revista Técnica de la Universidad de Zulia, 2020
A study was carried out in order to evaluate the germination, development and multiplication of Moringa oleifera Lam in vitro on different culture media, using seeds collected in an arid ecosystem. Murashige and Skoog (MS), Murashige and Skoog culture media and Gamborg (MSG), Water-Agar (0.8 %) and wet medium were evaluated in a completely randomized design with five replications. Variables related to germination, seedling development and its subsequent multiplication process were evaluated under in vitro conditions. The wet culture medium significantly outperformed (P≤0.05) the rest of the media in accumulated germination (100 %) and physiological indicators associated with germination. The weight of the seed was the one that most contributed to the germination process. There was a low (P≥0.05) percentage of contamination for all treatments. Likewise, in the growth and development of seedlings, the MS, Water-Agar and Wet media, stimulated (P≤0.01) the production of leaves (7.08 ± 1.2; 6.62 ± 0.7; 5.50 ± 0.9, in order) compared to the MSG (4.8 ± 2.2), however, the latter stimulated more (P≤0.01) number of shoots (0.20 ± 0.5) than the media, wet and Water-Agar and developed seedlings with longer roots (76.92 ± 17.6 mm) than the MS (P≤0.05). For the multiplication, only the MS and MSG media with the addition of 4.44 µL.mL-1 of 6-BA allowed the production of explants, the MSG provided the highest (P≤0.01) value with 31.54 % of explants. MS and MSG media are not necessary in seed germination under in vitro conditions, but essential in the growth process, development, multiplication of apical shoots.
AIP Conference Proceedings (Vol. 2606, No. 1). AIP Publishing., 2023
Moringa (Moringa oleifera Linn.) is a popular plant as a functional food crop. In micropropagation, modifying the medium culture is critical to determine the best nutrient composition for plantlet production. The antecedent research showed that the basic DKW (Driver and Kuniyaki Walnut) medium was the best compared to MS (Murashige and Skoog), WPM (Woody Plant Medium), NN (Nitsch and Nitsch), and B5 (Gamborg) media. This experiment aimed to enhance the growth of shoot culture and its root formation on a basic DKW medium supplemented with cytokinins and auxins. Two experiments were conducted. The first experiment was designed to obtain the best medium for shoot growth by adding cytokinin BAP, Kinetin, 2-IP, and Zeatin. The use of IAA (indole-3-acetic acid), NAA (1-naphthalene acetic acid), and IBA (3-indolebutyric acid) to enhance root formation was set for the second experiment. A completely randomized design with two elements was utilized for the experiment. The variables tried were: Plant Growth Regulators (PGR): BAP, Kinetin, 2-IP, and Zeatin with 0.0, 0.5, 1.0, and 2.0 mg/l for shoot development and IAA, NAA, and IBA at a similar concentration for root arrangement. Every treatment comprised of 12 recreates. The factors noticed were shoot stature, shoot numbers, petiole numbers, root numbers, and root length noticed one time per week. Following a month and a half after culture, plantlet performance were recorded. The best shoot stature, shoot numbers, and petiole numbers were created on a medium enhanced with 1 mg/l Kinetin and significantly unique with others. The most significant roots and root length were created at 1.0 and 2.0 mg/l IAA.
3 Biotech
The present investigations were attempted to develop the rapid in vitro micropropagation protocol of Moringa oleifera (Variety-PKM-1) from nodal sections of young, aseptically grown seedlings. Benzyladenine (BA) at 4.44 lM was found to be optimal in producing on maximum an average of 9.0 ± 1.0 axillary shoots per explant after 15 days of inoculation. A high multiplication rate was established through routine sub culturing of nodal sections explanted from in vitro shoot cultures. In vitro rooting of individual shoot culture was maximum (100%) on medium containing indole-3-acetic acid (IAA) at 2.85 lM along with indole-3-butyric acid (IBA) at 4.92 lM. Eighty percent of the rooted plants survived after being transplanted in the soil, provided that the potted plantlets were covered with clear polythene bags and kept in a shaded greenhouse for 15 days before exposure to ambient conditions. Fresh leaves of field grown tissue culture plants were analyzed for lutein, b-carotene, a-tocopherol, total carotenoids and chlorophyll content. Tissue culture-derived plants were found nutritionally superior over control plants to contain 13.2 and 14.7% higher amount a-tocopherol and total carotenoids, respectively. The result of present study will be useful for rapid clonal propagation of M. oleifera and production of nutritionally superior plant. Keywords Carotenoids Á Drumstick Á Horseradish tree Á Micropropagation Á Tocopherol Abbreviations BA Benzyladenine IAA Indole-3-acetic acid IBA Indole-3-butyric acid MS Murashige and Skoog NAA a-naphthalene acetic acid TRIA Triacontanol
Optimization of growth regulators on in vitro propagation of Moringa stenopetala from shoot explants
BMC Biotechnology
Background Moringa stenopetala belongs to the flowering family Moringaceae and genus Moringa. It is often referred to as the East African Moringa tree because it is native only to southern Ethiopia and northern Kenya. The expansion of its cultivation and utilization throughout the world especially in Africa is becoming important. For such expansion, the existing propagation method is limiting, so it needs a good propagation system to supply enough planting material with a uniform genotype. Therefore, the main objective of this study was to optimize an in vitro shoot multiplication protocol for M. stenopetala by using shoot tip as explants. Results Shoots were sterilized and cultured on Muraghige and Skoog (MS) medium for in vitro shoot initiation. For multiple shoot induction, the explants were cultured on MS medium supplemented with different concentrations of kinetin (0.5, 1.0, 1.5, 2.0, 2.5 mg/L) with Indole-3- butyric acid (IBA) or α -naphthalene acetic acid (NAA) (0.01, 0.1, 0....
AN EFFICIENT IN VITRO PROPAGATION PROTOCOL FOR MORINDA CITRIFOLIA L., AN IMPORTANT MEDICINAL PLANT
European Journal of Biomedical AND Pharmaceutical sciences, 2017
An efficient protocol was developed for in vitro propagation of Morinda citrifolia L., a traditional folk medicinal tree with pharmacological value and low seed germination capacity. A source of several phytochemicals and potent anti-microbial activities, development of a micropropagation protocol, will serve as a tool for exploiting the production of phytochemicals in vitro. The effect of different concentrations of plant growth regulators such as cytokinins (BAP and Kinetin) on shoot proliferation and auxins (IBA and NAA) and different strength of MS medium (Full, ½ and ¼ MS) on rooting were investigated. Cytokinin BAP promoted shoot proliferation more favourably than kinetin. Endogenous auxin levels indicated that rooting was induced without the exogenous supply of growth regulators and quarter strength MS basal media was sufficient for rooting of microshoots. Hardening and acclimatization of the plantlets were successfully with over 96%, exhibiting normal development. Synthetic seeds of M. citrifolia were also produced using nodal segments and shoot tips with sodium alginate, as a method for germplasm conservation.
2012
Morinda reticulata Gamble is a highly endangered medicinal plant of Southern Western Ghats of India. Since conventional propagation methods are not effective, in vitro propagation was attempted for the first time using nodal explants. Year long experiment on responses of apical bud and nodal explants revealed that summer season (March - May ) induced early bud break and faster shoot regeneration than in the other seasons. MS medium supplemented with 6-benzylaminopurine (BAP) and kinetin (KN) were tested at various concentrations ranging from 2.2muM - 46 muM. The maximum response of explants (62.7%) with longest mean shoot length (6.7 cm) was obtained with 4.4 muM BAP. The number of shoots per explant never increased beyond four even at higher levels of cytokinin However, healthy microshoots with uniform shoot development from the nodal segments led to the establishment of a large number of shoots. A combination of 0.12 mM silver nitrate with 22 muM BAP resulted in 4-5 shoots per nod...