Phytogenic Silver Nanoparticle Synthesis with Potential Antibacterial Activity and Dye Degrading Ability (original) (raw)
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J. Chem. Bio. Phy. Sci. Sec. B, 2019
Nanotechnology introduced the concept of nanoparticles, which exhibit unique and remarkably different properties from their bulk materials. Chemical methods are widely used among various strategies of synthesizing nanoparticles. Green synthesis is utilized as a cost effective and environmentally friendly alternative to the chemical method of synthesizing nanoparticles. Plant mediated synthesis of the nanoparticles is recognized as most promising green synthesis method and researchers have paid closer attention to the green synthesis of silver nanoparticles due to their remarkable antimicrobial activity. The review focus on the recent studies and present the methodology used in bio-reduction of silver nitrate solution using plant extracts, methods used in characterization of synthesized nanoparticles, various antibacterial assays conducted and also giving a brief overview of the results obtained from them
Bioengineering
Aloe vera, Mentha arvensis (mint), Coriandrum sativum (coriander), and Cymbopogon citratus (lemongrass) leaf extracts were used to synthesize stable silver nanoparticles (Ag-NPs) by green chemistry. UV–vis spectrophotometry, X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy techniques were used to characterize these biosynthesized nanoparticles. The data indicated that the silver nanoparticles were successfully synthesized, and the narrower particle size distribution was at 10–22 nm by maintaining a specific pH. As a short-term post-sowing treatment, Ag-NP solutions of different sizes (10 and 50 ppm) were introduced to mung bean seedlings, and the overall increase in plant growth was found to be more pronounced at 50 ppm concentration. The antibacterial activity of Ag-NPs was also investigated by disc diffusion test, minimum inhibitory concentration (MIC), and minimum bactericidal concentrati...
GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM SELECTED PLANT SPECIES AND THEIR ANTIBACTERIAL EFFECT
Green synthesis of silver nano particles from selected plant species and there anti bacterial activity , 2023
Water-borne pathogen contamination in water resources and related diseases are a major water quality concern throughout the world. Increasing interest in controlling water-borne pathogens in water resources evidenced by a large number of recent publications clearly attests to the need for studies that synthesize knowledge from multiple fields covering comparative aspects of pathogen contamination, and unify them in a single place in order to present and address the problem as a whole. Convectional water purification or disinfection techniques, such as chemical treatment, water treatment ozone or chlorine-based components lead to generation of disinfection byproducts. Antimicrobial activities of nano materials have the potential to avoid problems associated with the release of disinfection byproducts. The study reports a versatile, costefficient and eco-friendly protocol for the synthesis of biogenic silver nanoparticles (AgNPs) using the aqueous extracts of selected plant species: that is Bidens pilosa and Plectranthus cyaneus, partial characterization and their anti-bacterial activity. Leaves of the named plant species were collected, washed, cut into pieces and crushed and placed in a 250ml Erlenmeyer flask containing 100 ml distilled water and boiled for 20 minutes. It was the filtered and the filtrate was reacted silver nitrate to synthesize AgNPs. The biosynthesized nanoparticles (AgNPs) were characterized by UV-spectroscopy and Fourier-transform infrared spectroscopy (FTIR). AgNPs were evaluated for their antimicrobial potentials. The UV-Vis spectra showed a complete reduction of Ag+ to Ag0 with Plasmon resonance bands at around 430 nm which is characteristic of AgNPs. FT-IR of the synthesized AgNPs revealed the presence of functional groups: C-O, O-H, =C-H, aromatic C-H bending, C=O, and C=C aromatic attatched to silver nanoparticles. The synthesized AgNPs were observed to possess high activity against Escherichia coli and Staphylococcus aureus respectively. Results of the study revealed that AgNPs exhibited high antibacterial potentials. The antibacterial activity of the biogenic nanoparticles would find applications in the biomedical and environmental fields.
Synthesis and Antimicrobial Activity of Plant Based Silver Nanoparticles - A Review
Bioscience Biotechnology Research Communications, 2020
Nanotechnology is an exciting and influential world of manufacturing nanoparticles that find varied medicines, bacterial studies, wastewater treatment, and many more applications. This review paper aims to review the synthesis of silver nanoparticles from horticultural sources and evaluate special applications causing a considerable impact on the environment. Conventionally silver nanoparticles are produced by various chemical and physical processes. Biosynthesis of silver nanoparticles offers alternative methods, where plant extract can be used instead of harmful chemicals. Among various nanoparticles, silver nanoparticles possess unique antibacterial and antiviral properties make them superior to other nanoparticles. The green chemistry approach for synthesizing silver nanomaterials is environmentally friendly, benign, and gaining wide popularity. Enzymes, proteins, phytochemicals, phytochelatin, and vitamins are biomolecules found in plant extracts. In the process of biosynthesis of AgNPs, plant extracts act as good reducing, capping agents.. Biomolecules help reduce and stabilize the process of silver nanoparticles. The biomolecules enhance the yield process of biosynthesis of AGNP. This review highlights a summary of silver nanoparticle biosynthesis and their antibacterial activity. Silver nanoparticles is a multidisciplinary area that has gained focus from researchers of diverse areas from science, pharmaceuticals, agriculture, and materials engineering due to their morphologies and composition. Various physical and chemical techniques for synthesis include biological methods, chemical reduction, electron irradiation, gamma irradiation, laser ablation, microwave processing, and photochemical methods. The authors have attempted to compile the reported studies regarding silver nanoparticle synthesis from plants and their specific antibacterial properties. This paper reviews various synthesis techniques from plant materials and medical applications of silver nanoparticles. An attempt is made to present the current state and future silver nanoparticle applications in general and antibacterial treatments in particular.
Antimicrobial activity of silver nanoparticles synthesized by using medicinal plants
Int. J. Chem. Tech. …, 2011
Biologically synthesized silver nanoparticles (SNPs) are being widely using in the field of medicine. Extracellular biosynthesis of silver nanoparticles was carried out by using medicinal plant extracts for the reduction of aqueous silver ions in short period. The silver nanoparticles formation was confirmed by the colour change of plant extracts (SNPs) and further confirmed with the help of UV-Vis spectroscopy. These Phytosynthesized silver nanoparticles were tested for antibacterial and antifungal activities using disc diffusion method. The test cultures are Proteus, Pseudomonas, Klebsiella, Bacillus and E.coli species of bacteria and Aspergillus, Fusarium, Curvularia and Rhizopus species of fungal were used. The microbial property of silver nanoparticles was analyzed by measuring the inhibition zone. The silver nanoparticles synthesized from stem bark extracts of Boswellia and Shorea; and leaf extract of Svensonia. The SNPs synthesized from bark extracts of Boswellia ovalifoliolata and Shorea tumbuggaia showed toxic towards Klebsiella and Aspergillus; and Pseudomonas and Fusarium species respectively. Whereas the growth of Pseudomonas and Rhizopus species were inhibited maximum by the SNPs synthesized from leaf extract of Svensonia hyderobadensis, the results indicate that the silver nanoparticles may have an important advantage over conventional antibiotics.
Applied Biochemistry and Microbiology
screening of aqueous extracts of herbaceous plants that grow on the territory of the Russian Federation with their subsequent use for the biosynthesis of silver nanoparticles (SNPs) in accordance with the principles of green chemistry has been performed. Extracts from the leaves of three plants (Mentha piperita L., Melilotus officinalis, and Archangelica officinalis) promoted efficient synthesis of SNPs, reducing the silver cation from AgNO 3. SNP formation was tested on a spectrophotometer. Atomic force microscopy showed that plant extracts cause the formation of SNPs of different sizes-from 10 to 80 nm. Scanning electron microscopy revealed SNPs with various shapes and sizes; they were most commonly spherical. The obtained nanoparticles had a bactericidal effect on Escherichia coli K-12 and Pseudomonas aeruginosa PA01 (the latter being more resistant), and they suppressed the formation of E. coli biofilms. The obtained data show that the use of extracts from three herbaceous plants provides a readily available and ecologically safe method for the production of SNPs with antimicrobial activity.
Journal of AOAC International, 2018
Background: Silver nanoparticles (AgNPs) are employed in various applications in the areas of catalysis, optoelectronics, detection and diagnostics, antimicrobials, and therapeutics. Objective: The aim of this work was to study the antimicrobial activity of aqueous and methanolic leaf extracts of Thymus vulgaris and Urtica dioica and biologically prepared silver nanoparticles, as single or in combination treatments, against Escherichia coli and methicillin-resistant Staphylococcus aureus isolates. Methods: The minimum inhibitory concentration (MIC) was quantified by using a microdilution method in sterile 96-well microtiter plates. The assessment of the toxicity of AgNP solutions was evaluated on human blood lymphocyte cells. Results: The results of this study revealed that all AgNP solutions have the lowest MIC values against the bacterial isolates in relation with the methanolic and aqueous extract solutions. However, the results showed that the increasing AgNP concentration was a...
Plant-mediated Biosynthesis of Silver Nanoparticles and Evaluation of Their Antibacterial Activities
Aqueous seed extract of Syzygium cumini (SCE) was used for the synthesis of silver (Ag) nanoparticles. The nanoparticles were characterized with the help of UV–vis spectrophotometer, Particle size analyser (PSA), Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). UV-Vis spectra of silver nanoparticles shows absorbance peak at 440 nm. The nanoparticles synthesized were relatively spherical in shape with varying sizes. The biosynthesized silver nanoparticles were found to be highly toxic against some human pathogenic bacteria.
Antibacterial and Cytotoxic Potential of Biosynthesized Silver Nanoparticles by Some Plant Extracts
Nanomaterials
The provision of nanoparticles using biogenic material as a part of green chemistry is an attractive nanotechnology. The current research aimed to test the antimicrobial and cytotoxic efficacy of silver nanoparticles synthesized by extracts of Phoenix dactylifera, Ferula asafetida, and Acacia nilotica as reductant and stabilizing agents in silver nanoparticle formation. Synthesized nanoparticles were evaluated for their antimicrobial activity against Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa and Escherichia coli (Gram-negative) using an agar well diffusion assay. Furthermore, cytotoxic ability was investigated against LoVo cells. The potential phyto-constituents of plant extracts were identified by Fourier-transform infrared spectroscopy (FT-IR) techniques. Field emission scanning electron microscopy (FE-SEM), transmission electron microscope (TEM), and zeta potential analyzed the size and morphology of the biogenic nanoparticles. The current study revealed the ability of the tested plant extract to convert silver ions to silver nanoparticles with an average size that ranged between 67.8 ± 0.3 and 155.7 ± 1.5 nm in diameter. Biogenic AgNPs showed significant antibacterial ability (10 to 32 mm diameter) and anticancer ability against a LoVo cell with IC50 ranged between 35.15-56.73 µg/mL. The innovation of the present study is that the green synthesis of NPs, which is simple and cost effective, provides stable nano-materials and can be an alternative for the large-scale synthesis of silver nanoparticles.
Journal of Genetic Engineering and Biotechnology
Background The Ag-NPs by green synthesis has a notable interest because of their eco-friendliness, economic views, feasibility, and applications in a wide range. Herein, native plants of Jharkhand (Polygonum plebeium, Litsea glutinosa, and Vangueria spinosus) were selected for the current work of Ag-NP synthesis and further antibacterial activity. Green synthesis was performed for Ag-NPs using Silver nitrate solution as precursor and the dried leaf extract performs as a reductant and stabilizer here. Result Visually Ag-NP formation was observed along with a colour change and confirmed by UV-visible spectrophotometry on which an absorbance peak occurs at around 400–450nm. Further characterization was done on DLS, FTIR, FESEM, and XRD. Size around 45–86 nm of synthesized Ag-NPs was predicted through DLS. The synthesized Ag-NPs exhibited significant antibacterial activity against Bacillus subtilis (Gram-positive bacteria) and Salmonella typhi (Gram-negative bacteria). The finest antiba...