Novel CMS lines in pigeonpea [Cajanus cajan (L.) Millspaugh] derived from cytoplasmic substitutions, and their effective restoration and deployment in hybrid breeding (original) (raw)
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Research Article Inheritance study of pollen fertility restoration of CMS lines in pigeonpea
Segregating patterns for pollen fertility of five crosses were studied involving two CGMS lines and four restorers viz., ICPA 2043/ICP 6399, ICPA 2043/ICP 9149, ICPA 2043/ICPR 4105, ICPA 2092/ICPR 4105 and ICPA 2092/KA 91-25 in F 2 and BC 1 F 1 generations. The crosses exhibited 13: 3 and 3:1 ratios, respectively in F2 and BC1F1 indicating the involvement of two dominant genes with one basic and one inhibitory gene action for fertility restoration. However, three crosses, ICPA 2092/ICP 6399, ICPA 2092/ICP 9149 and ICPA 2043/KA 91-25 recorded the ratio of 9 : 3 : 4, 12 : 3 : 1 and 9 : 7 and 1 : 1 : 2, 2 : 1 : 1 and1 : 3 in F 2 and BC 1 F 1 populations, respectively and these are confirming that supplementary, masking and complimentary gene actions. Introduction Pigeonpea [Cajanus cajan (L.) Millspaugh] (2n=2x=22) is a food legume crops which belong to the family Fabaceae and is invariably cultivated as an annual crop. It is an often cross-pollinated (20-70%) crop, globally grown on 4...
Genetics of fertility restoration and agronomic performance of CMS based hybrids in pigeonpea
In the study, CMS based hybrid combination involving two male sterile lines (Cajanus cajanifolius) and four restorer lines in pigeonpea (Cajanus cajan) were studied to determine the genetics of fertility restoration in pigeonpea (Cajanus cajan L. Millsp.). Based on the pollen fertility and pod setting, F 2 segregating populations were categorised. Out of two major genes governing the fertility restoration, one gene segregated in the ratio of 9:3:4 whereas the second gene in 12:3:1 due to the allelic differences. The ICPA 2043/ LRG 41 and ICPA 2043/NDA 1 hybrids fits best for agronomical aspects as they showed earliness with respect to flowering and maturity having more number of pods plant-1 , increase in pod length along with more seed yield plant-1. CMS system is a useful tool serving to increase seed yield production of various crops and consummate the demands of increasing populations. Highlights • Fertility restoration was observed to be governed by two major genes. • The seed yield of hybrids was extremely high i.e. 62.12 and 12.18 and 67.32 and 15.78 per cent over the checks LRG 41 and NDA1 respectively.
American Journal of Plant Sciences, 2015
Exploitation of hybrid vigour has been visualized as the most efficient option for increasing productivity in pigeonpea [Cajanus cajan (L.) Millspaugh]. Cytoplasms from various wild relatives of pigeonpea have been transferred to develop CMS lines in the background of cultivated pigeonpea. However, A 2 (Cajanus scarabaeoides) and A 4 (Cajanus cajanifolius) cytoplasms have been utilized most frequently. In order to study fertility restoration efficiency in F 1 hybrids having either A 2 or A 4 cytoplasms, an experiment was conducted at the Indian Institute of Pulses Research (IIPR), Kanpur during 2008-2012. Four CMS lines namely Hy4A, H28A (each with A 2 cytoplasm), ICP 2039A and ICP 2043A (both with A 4 cytoplasm) were crossed with ten genotypes/restorers of long duration pigeonpea for two years. The F 1 hybrids so-obtained were assessed in the succeeding years for pollen fertility and pod setting. All the pollinators except IPA 203 restored fertility in F 1 hybrids derived from ICP 2039A and ICP 2043A (both having A 4 cytoplasm). However, none of the restorers were effective in restoring fertility in hybrids derived from Hy4A and H28A (each with A 2 cytoplasm). This could be ascribed to undesirable linkage drag still present in these two CMS lines having A 2 cytoplasm. The F 2 progenies derived from 4 hybrids (ICP 2039A × NA-1, ICP 2039A × Bahar, ICP 2043A × NA-1 and ICP 2043A × Bahar) segregated approximately into 3 fertile: 1 sterile plants. However, 2 F 2 progenies having Pusa 9 as the restorer revealed approximately 15 fertile:1 sterile ratio. Thus monogenic and digenic duplicate gene action with complete dominance for fertility restoration was observed in F 1 hybrids derived from CMS lines having A 4 cytoplasm. F 3 progenies from individual F 2 plants of these crosses also confirmed the same pattern of fertility restoration. This study indicated that CMS lines based on A 4 cytoplasm would be more desirable as these might have more number of restorers compared to those having A 2 cytoplasm.
Inheritance of fertility restoration in pigeonpea
Journal of Food Legumes, 2011
Fertility restoration system in five CMS-based pigeonpea [(Cajanus cajan (L.) Millspaugh] hybrids was studied during kharif 2010 at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Andhra Pradesh. Two hybrids ‘ICPH 2671’ and ‘ICPH 2740’ which had the same male parent but different females segregated in F2 in the ratio of 12 fertile (F): 3 partial fertile (PF):1 sterile (S), and in BC1F1 generation as 2 fertile: 1 partial fertile: 1 sterile, suggesting that fertility restoration in these hybrids was controlled by digenic dominant epistatic interaction. The progenies derived from hybrid ‘ICPH 3359’ fitted well to an F2 ratio of 9 F: 6 PF: 1 S, and 1 F: 2 PF: 1 S in BC1F1 generation, indicating the involvement of two major genes with incomplete dominant epistasis. Progenies of the other two hybrids ‘ICPH 4012’ and ‘ICPH 4344’ segregated in F2 in the ratio of 9 F: 3 PF: 4 S, and 1 F: 1 PF: 2 S in BC1F1 generations, suggesting that pollen fertility ...
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2018
We report molecular mapping and inheritance of restoration of fertility (Rf) in A4 hybrid system in pigeonpea. We have also developed PCR-based markers amenable to low-cost genotyping to identify fertility restorer lines. Commercial hybrids in pigeonpea are based on A4 cytoplasmic male sterility (CMS) system, and their fertility restoration is one of the key prerequisites for breeding. In this context, an effort has been made to understand the genetics and identify quantitative trait loci (QTL) associated with restoration of fertility (Rf). One F population was developed by crossing CMS line (ICPA 2039) with fertility restorer line (ICPL 87119). Genetic analysis has shown involvement of two dominant genes in regulation of restoration of fertility. In parallel, the genotyping-by-sequencing (GBS) approach has generated ~ 33 Gb data on the F population. GBS data have provided 2457 single nucleotide polymorphism (SNPs) segregating across the mapping population. Based on these genotyping...
Journal of Applied Genetics, 2008
Exploitation of hybrid vigour is quite possible in cross-pollinated crops. However, pigeonpea is a grain legume crop with a moderate level of cross-pollination (20–70%), which is mainly aided by insect pollinators. As a first step, hybrids based on genetic male sterility (GMS) were developed in pigeonpea, but the hybrid seed production technique is not farmer-friendly, because in the hybrid seed production plot 50% of the population, which are male-fertile in the female rows, have to be eliminated in time before contamination. This requires skilled labour and is a time-consuming process, which increases the cost of hybrid seed production. Therefore, the objective of this study was to develop cytoplasmic-genetic male-sterile (CGMS) lines in pigeonpea through wide hybridization, which would be very suitable for hybrid seed production. Two CGMS lines, viz. CORG 990052 A and CORG 990047, were developed by interspecific hybridization ofCajanus cajan andC. scarabaeoides. Restorers were identified and three CGMS-based pigeonpea hybrids were developed. The hybrid COPH 3 is found to be promising in Tamil Nadu State, India.
RAPD-based polymorphism between cytoplasmic genic male sterile and restorer lines of pigeonpea
The cytoplasmic-genetic male sterility (CMS) system is considered to be feasible approach to develop hybrids in pigeonpea. Identification of CMS lines and their putative restorers using molecular markers in the early stage of growth is important and economical in pigeonpea. The RAPD analyses of genomic DNA were carried out using 80 RAPD primers in ten different genotypes each of A and R lines of pigeonpea. 76 of the 80 RAPD primers produced clear and unambiguous banding pattern among A and R lines. Out of these, 72 were polymorphic and 4 were monomorphic. A total of 702 bands were amplified, out of which 544 were polymorphic. This evinced on an average 7.55 polymorphic bands/primer; though the average number of amplified bands per primer were 8.77. The number of amplified loci varied from two each in OPC2, OPC12, OPB1, OPB5, OPB6 and OPB9 to twenty two in OPA 1 andOPA19. The size of amplified bands ranged from 100 bp to 2850 bp. The polymorphic 1.1kb fragment amplified by OPC1 was s...
Electronic Journal of Plant Breeding
In pigeonpea the cytoplasmic-genetic male sterility (CMS) system is considered to be a feasible approach for the development of hybrids. Identification of CMS lines and their putative restorers using molecular markers in the early stage of growth is an important and economical in long duration pigeonpea. Based on phenotyping, the 120 plants of F 2 designated as fertile and sterile. Both visual and pollen staining phenotyping unequivocally exhibited 85 fertile: 35 sterile plants in F 2 population. The segregation of F 2 for fertility restoration of A 2 cytoplasm indicated 3F:1S segregation indicating dominant monogenic control of fertility restoration. Bulk segregant analysis revealed that 10 of the 139 polymorphic decamer RAPD markers produced precisely distinct bands subjective to fertility restorer DNA bulk while, none of the SSR was found polymorphic between bulks. Two of them viz; OPC 7 617 (GTC CCG ACG A) and OPK 3 860 (CCA GCT TAG G) evinced clear and unambiguous bands for fertility restorations in F 2 population at 0.5 and 1.0 cM, respectively. They could be exploited as a precise selection tool for seminal but stubborn character like restoration of fertility in male sterile lines that has germane significance in otherwise immensely important heterosis breeding in pigeonpea.
Hybrid pigeonpea [Cajanus cajan (L.) Millsp.] breeding technology based on cytoplasmic-genetic male sterility (CMS) was recently developed ICRISAT, Patancheru in India. A program was initiated to track the purity of female parental lines by incorporating an obcordate leaf shape marker in established male sterile A-lines. Seven obcordate A-lines developed by backcrossing and selection were crossed with four known fertility restorers in line x tester mating design to study their general and specific combining ability. Higher magnitude of specific combining ability (SCA) effect showed that, hybrid yield was under the control of non-additive genes. Among A-lines, ICPA 2204 was the best general combiner. Among testers, ICPL 20116 was the best general combiner. Among hybrids, ICPA 2208 x ICPL 20108 a cross between high general combining ability (GCA) parents was the best with positive significant SCA effect and higher mean performance for grain yield, 100-seed mass, number of seeds/pod an...