Evaluation of antibacterial activity of Zizyphus jujuba (original) (raw)
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Antibacterial activity of Zizyphus
2020
Red date or Bera (Pushto), is used primarily for its fruits. Jujube, a delicious fruit, is an effective herbal remedy improving stamina and muscular strength and aids weight gain, strengthens liver function and increases immune system resistance. The aim of this study is to evaluate in-vitro efficacy of antibacterial activity of crude methanol, n-hexane, chloroform, ethyl acetate and aqueous extracts of Zizyphus jujuba against some human pathogenic bacterial strains. Antibacterial and antifungal activities of Zizyphus jujuba extract was carried out by using disc diffusion method. The crude methanol, n -hexane, chloroform, ethyl acetate and aqueous fractions showed 41.37, 44.82, 41.37, 55.17 and 44.82% activity against Enterobacter aerogenes respectively. The crude methanolic extract and n-hexane fractions were inactive against Escherichia coli, S. pneumoniae and Klebsella pneumonia respectively. From this study, the extracts namely n-hexane, ethyl acetate and aqueous extracts of Zizyphus jujuba possess antibacterial activity
Study of Antimicrobial Activity of ZiziphusJujuba Leaf, Fruit and Seed Extracts
International Journal of Advanced Science and Engineering, 2023
Nearly 60% of the world’s population and pharmaceuticals depends on natural plants as a source for treating human ailments. In this context, Ziziphus jujube has been called a multipurpose tree from time immemorial as it has immense medicinal and Phytopharmacological properties. The present study was involved in establishing the antimicrobial effect of the leaves, seeds, and fruits of Ziziphusjujuba. Hence, the crude extracts of different parts of the above-mentioned multipurpose tree had been tested to determine their antimicrobial nature against two gram-positive and five gram- negative bacterial species in which all the extracts showed more potent antibacterial activity against gram-negative bacterial species than gram-positive bacteria. The crude extracts obtained from the leaf, seeds and fruits of Ziziphus jujube were also tested to determine its antifungal activity against Candida albicans, Aspergillusniger, and Trichopytontonsurans. The results stated that all three crude extracts were effective against Candida albicans and Aspergillusniger when compared with Trichopytontonsurans.
Screening of Zizyphus jujuba for antibacterial, phytotoxic and haemagglutination activities
African Journal of …, 2011
Keeping in view this idea, the crude methanolic extract and various fractions of Zizyphus jujuba were screened for antibacterial, phytotoxic and haemagglutination activities. The n-hexane and aqueous fractions showed significant activity of 60 and 66.66% against Bacillus pumalis and Pseudomonas aerugenosa, respectively. Activity of ethyl acetate fraction was 65.38, 62.96, 62.96 and 72% against Staphylococcus epidermidis, Salmonella typhi, P. aerugenosa and B. pumalis, respectively. On the contrary, no activity of this fraction was recorded against Streptococcus pneumoniae. The crude, nhexane, chloroform, ethyl acetate and aqueous fractions showed 41.37, 44.82, 41.37, 55.17 and 44.82% activity against Enterobacter aerogenes, respectively. The crude methanolic extract and n-hexane fractions were inactive against Escherichia coli, S. pneumoniae and Klebsella pneumoniae, respectively. The ethyl acetate fraction was moderately phytotoxic against Lemna minor L at 1000 µg/ml. All the other fractions showed low phytotoxic activity at 1000 µg/ml. At 100 µg/ml, all the fractions showed low phytotoxic activity except crude methanolic extract, which was inactive. All the test samples were inactive at 10 µg/ml. All dilutions of the test samples showed no haemagglutination activity against any blood group.
The purpose of the present study was to investigate the antioxidant and antimicrobial activities of a methanolic extract of Apple kul (Zizyphus mauritiana) as it has not been studied extensively. Apple kul was found to be a rich source of polyphenols (52.19 ± 2.38 mg gallic acid equivalents/100 g), flavonoids (13.19 ± 1.31 mg catechin equivalents/100 g), ascorbic acid (48.17 ± 2.04 mg ascorbate equivalent/100 g) and tannins (50.20 ± 3.61 mg tannic acid equivalents/ 100 g). The estimated protein and reducing sugar contents in Apple kul were 1.21 ± 0.04 g/100 g and 1.96 ± 0.15 g/100 g, respectively. The high ferric-reducing antioxidant power value (6336.71 ± 554.88 μmol Fe [II]/g) also indicated a high antioxidant potency for Apple kul. Apple kul showed highest activity towards Pseudomonas aeruginosa and Staphylococcus aureus.
Investigations on Ziziphus Jujuba Seed Extract for Antimicrobial Activity -- A Pilot Study
2021
The aims of this study were to test the antibacterial activity and chemical composition of Ziziphus jujuba extract. The extract was obtained using 50% aqueous-ethanol extraction solution to extract Ziziphus jujuba seeds. The extract was prepared and evaluated for antimicrobial activity against six bacterial strains by determining minimum inhibitory concentration (MIC). The results revealed that the 50% aqueous ethanol extract is potent in inhibiting bacterial growth of both gram-positive and gram negative bacteria. The chemical composition of fenugreek was analyzed by gas chromatography/mass spectroscopy. The 13-Heptadecyn-1-ol (12.95%), 7-Ethyl-4-decen-6-one (9.73%), Lineoleoyl chloride (8.54%), Linoleic acid (6.37%), 2,5-Octadecadiynoic acid, methyl ester (5.57%) and Palatinol A (4.81%) were the highest abundant compounds out of total 20 compounds were identified in the Ziziphus jujuba seeds extract. This research work deals with the detailed pharmacognostical evaluation of the crude drug. The physico-chemical and histological parameters presented in this paper may be proposed as parameters to establish authenticity of Ziziphus Jujuba and can possibly help to differentiate the drug from its other species. As there is no pharmacognostical anatomical work on records for this traditionally valued herb, the present study is taken up in the view to lay down the physico-chemical and microscopic standards.
Crude Extract from Ziziphus Jujuba Fruits, a Weapon against Pediatric Infectious Disease
Iranian journal of pediatric hematology and oncology, 2013
Pediatric infectious disease is one of the main problems in cancerous children that treat by chemotherapy drugs. Thus, study in this regard is necessary. The aim of this study was to evaluate antimicrobial properties of ethanolic extract of Ziziphus Jujuba fruits against different infectious pathogens. This study is descriptive. In vitro antimicrobial activity of extract was assessed on gram negative and gram positive bacteria as well as fungi. The antimicrobial activity was tested by Radial Diffusion Assay (RDA) and Minimal Inhibitory Concentration (MIC) methods. The results showed a wide antimicrobial activity of the extract against the microbes studied. Escherichia coli was the most susceptible to the extracts among tested microorganisms for which the MIC was 0.65±0.22 mg/ml. Amongst the bacterial strains investigated, Staphylococcus aureus was the most resistant strain with MIC of 2.26±0.68 mg/ml. The ethanolic extract also showed antimicrobial activity on the fungi studied as n...
Invitro-efficacy of crude extract of zizipus jujuba against selected bacterial strains
Abstract- The research was assessed to evaluate the efficacy of crude extract of Zizipus jujuba against selected bacterial strains. The extract of Zizipus jujuba leaves was obtained by three different methods and the inhibition zones obtained through disc diffusion method. A decent antibacterial activity of Zizipus jujuba leaves crude extract of cold water and ethanol was found against Salmonella typhimurium and Staphylococcus aereus. Maximum zone of inhibition through cold water extract was obtained by Enterococcus feacalis (32mm) followed by Staphylococcus aureus (28mm), Salmonella typhimurium (27.52), Klebsilla pneumonia (19mm) and Escherichia coli (19mm). Similarly maximum zone of inhibition through ethanol extract was obtained by Staphylococcus aureus (28mm) followed by Salmonella typhimurium (27.52). The bacterial species showed no sensitivity against hot water extract due to the degradation of alkaloids in hot water. Mean observation taken was that these bacterial species can be inhibited by Zizipus jujuba plant. The study showed that Zizipus jujuba plant can be used to obtained antibiotics having less or no side effect, especially against Salmonella and staphylococcus aureus infections.
Egyptian Journal of Botany
T HE ANTIBACTERIAL potential of Ziziphus-spina christi as methanol and ethanol extracts, of bark, fruit, roots, seeds and leaves, were evaluated against seven pathogenic bacterial strains using agar well diffusion technique: The used strains were Pseudomonas aeruginosa (ATCC 278223), Enterobacter cloacae (ATCC 13047), Enterobacter aerogenes (ATCC 13084), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 13888), Enterococcus faecalis (ATCC 29212) and Methicillin-resistant Staphylococcus aureus (MRSA, ATCC 43300). The antibiotic erythromycin was used as positive control. The presence of phytochemical compounds in the extracts was determined qualitatively, the functional bioactive groups were characterized by FTIR and the presence of bioactive elements was characterized by XRF. Antibacterial activity against the used bacterial strains was assessed by determining the minimal inhibitory and bactericidal concentrations (MIC and MBC) assays. All the used Gram negative and positive bacteria were sensitive to various plant extracts. Bark extract was the most active against all strains except for Enterobacter aerogenes. Ethanol bark extract showed great activity against Enterococcus faecalis with 16.2 mm clearing zone and MIC, MBC of 15 and 20 mg ml-1 , respectively. Methanol bark extract was also effective against Klebsiella pneumoniae with a clearing zone of 16.2 mm and 20 mg ml-1 for both MIC, MBC. Leaves extracts showed high antibacterial activity against all strains except Escherichia coli. Ethanol fruit extract also exhibited high activity against Pseudomonas aeruginosa with 20 mm clearing zone and 2.5, 10 mg ml-1 for both MIC and MBC. Finally, ethanol roots showed the largest clearing zone against Enterococcus faecalis (21mm) with 10 and 15 mg ml-1 for MIC and MBC, respectively.
Journal of Applied and Advanced Research, 2019
Despite tremendous progress in human medicines infectious diseases caused by bacteria, fungi, viruses and parasites are still a major threat to public health. Their impact is particularly large in developing countries due to relative unavailability of medicines and emergence of widespread drugs resistance (Zampini 2009).The aim and objectives of this research work was designed to carried out the Phytochemical analysis, proximate composition and evaluate the antimicrobial activities of the Ziziphus jujube and Ziziphus spina christi leaves against clinical bacterial isolates (Escherichia coli, Staphloccoccus aureus and Klebsiella pneumoniae) as they were found out that since ancient times to date, they are used in treating various antimicrobial, ailmentand disorders etc. The results of the research shows that, the effect of antibacterial activities of both aqueous and ethanolic extracts of Ziziphus jujube (e extract conc. aqueous; F=119.37, Bacterial extract conc. aqueous; F=1.00 an...
Objective: The objective of the study was to evaluate antipyretic activity of crude methanol extract of Zizyphus jujuba leaves. Z.jujuba commonly called, Red date or Chinese date or Bera (Pushto), belonging to family Rhamnaceae, is used primarily for its fruits. Jujube, a delicious fruit, is an effective herbal remedy improving stamina and muscular strength and aids weight gain, strengthens liver function and increases immune system resistance. Methodology: The anti-pyretic activity of methanolic extract of Zizyphus jujuba Lam leaves was evaluated to Brewer's yeast induced pyrexia in rats with respect to control group. Results: In the first hour, the antipyretic effect of methanol extract of Zizyphus jujuba (200 and 400 mg/kg) was significant (p<0.05, and p <0.01 respectively). The results suggests that Zizyphus jujuba at a dose of 200mg/kg caused a highly significant reduction at third hour (p<0.001). However, the effect increases significantly at the dose of 400 mg/kg having (p<0.01) at first, second and fourth hour. The antipyretic effect was comparable with that of a standard Paracetamol. Conclusion: The results of the study indicate that the MEZJ has better potential against Pyrexia which supports the traditional claims in folklore medicine. Phytochemical investigation suggests the presence of saponin in the leaves which may be responsible for the anti-ulcer activity.