Differentiation of Mesenchymal Stem Cells into Photoreceptor-like Cells under the Influence of a Temporary Increase in miRNA-182, -183 Expression (original) (raw)

2021

Abstract

It is found that the death of retinal photoreceptors is the main cause of retinal degeneration, while there is not an effective treatment protocol. Data of preclinical and clinical trials indicates that the stem cell therapy is a useful way of treating retinal degeneration problems. On the other hand, previous works found that miRNA-182, -183 significantly affected the photoreceptor maturation and maintenance in animal models. The present study aimed to investigate the impact of a temporary increase in miRNA-182, -183 expression on the differentiation of human bone marrow mesenchymal stem cells (hBMSCs) into photoreceptor-like cells. To this end, miRNA-182, -183 was transfected into hBMSCs; then, qRT-PCR was performed to measure the expression levels of miRNA-182, -183 and some retina-specific genes such as OTX2, NRL, PKCα, and recoverin. CRX and rhodopsin (RHO) levels were also measured through qRT-PCR and immunocytochemistry We indicated that the transfection of hBMSCs with miRNA-...

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