Cell viability, anti-proliferation and antioxidant activities of Sideritis syriaca, Tanacetum argenteum subsp. argenteum and Achillea aleppica subsp. zederbaueri on human breast cancer cell line (MCF-7) (original) (raw)
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Antiproliferative activity of plant extracts used against cancer in traditional medicine
Scientia pharmaceutica, 2010
Forty four extracts from sixteen plants used traditionally as anticancer agents were evaluated in vitro for their antiproliferative activity against Hep-2, MCF-7, and Vero cell lines. Plants were fractionated using ethanol, methanol, chloroform, n-hexane, distilled water, and butanol. The antiproliferative activity was measured by MTT assay. TLC was used to identify active fractions. The apoptotic activity of active fractions was determined using TUNEL colorimetric assay. 20 of these extracts demonstrated significant antiproliferative activity against one or more of the cell lines. These extracts were prepared from Ononis hirta, Inula viscosa, Salvia pinardi, Verbascum sinaiticum and Ononis sicula. Methanol fractions of Ononis hirta (aerial parts) and Inula viscosa (flowers) were the most active fractions against MCF-7 cells with IC(50) of 27.96 and 15.78 Îg/ml respectively and they were less toxic against other cell lines. Other extracts showed lower activity against cancer cell li...
Cellular and Molecular Biology, 2021
Cancer is the leading cause of death, accounting for approximately one out of six people dying with this disease worldwide. Among all, the breast and ovarian cancers are top-ranked causes of women mortalities compared to other disorders. Although, there is advancement in technologies, but still, there are unresolved concerns to overcome the global disease burden. Currently, plants are being explored as a natural remedy to cure disorders. This research was planned to explore phytochemicals in methanolic extracts of Zizyphus mauritiana and Triticum aestivum, and their pharmacological activities were studied through Agro-bacterium tumefaciens bacteria, in vitro breast cancer cell line and ovarian cancer cell line to find out novel candidates in disease control and prevention. Eleven different types of bioactive compounds were analysed in the tested extracts. The highest crude extracts percentage (75±0.02) was observed with Z. mauritiana. The extracts showed promising cell growth inhibition and tumor initiation inhibition in potato disc assay. MTT assay and Incucytes imaging analysis revealed that Z. mauritiana extract had a higher anticancer potential with 40 ± 0.92 cell viability against breast cancer cells (SKBR3) and 45 ±0.29 against ovarian cancer cells (SKOV3). In conclusion, these extracts could be used as chemotherapeutics owing to their cheapness, and easy availability. While detailed study is required for further purification and characterization of bioactives/target compounds and in-vivo activity confirmations.
A Review of Medicinal Plants Effective in the Treatment or Apoptosis of Cancer Cells
The Cancer Press, 2017
Cancer in our country is the most important public health problem. In addition to lifestyle changes and population aging, cancer seems to be much more important in the future (1). medicinal plants are traditionally used to treat many ailments, including cancer and related diseases. Cancer is characterized as a condition with complex signs and symptoms. It has been recommended that ethnopharmacological usages such as immune and skin disorders, inflammatory, infectious, parasitic and viral diseases should be taken into account when selecting plants for anticancer screenings, since these reflect disease states bearing relevance to cancer or cancer-like symptoms (2). By definition, 'traditional' use of herbal medicines implies substantial historical use, and this is certainly true for many products that are available as 'traditional herbal medicines'. In many developing countries, a large proportion of the population relies on traditional practitioners and their armamentarium of medicinal plants in order to meet health care needs. Although modern medicine may exist side-by Medicinal herbs in various fields of medicine, industry, food and agriculture applications. In the field of medicine and therapy for the treatment of cancer researchers hope many medicinal plants. Plants, herbs, and ethnobotanicals have been used since the early days of humankind and are still used throughout the world for health promotion and treatment of disease. Plants and natural sources form the basis of today's modern medicine and contribute largely to the commercial drug preparations manufactured today. About 25% of drugs prescribed worldwide are derived from plants. Still, herbs, rather than drugs, are often used in health care. For some, herbal medicine is their preferred method of treatment. Today, natural antioxidants are the focus of considerable attention and efforts are ongoing for the replacement of synthetic ones. In addition, these natural antioxidants can be formulated as functional foods and can help prevent oxidative damage from occurring in the body. Due to the side effects of drugs and chemicals in countries around the world, including developed countries are thinking about changing pattern of drug use of chemical plant. Some active drug substances which are very important in the pharmaceutical industry, is impossible to artificially produce only natural as Astkhrajand plants. Although synthetic medicines to improve patients more quickly and has an adverse effect on the human body Mshkhsand but most of them can have side effects. In this study, we report and review of some medicinal plants effective in the treatment of cancer or other diseases discussed.
In-Vitro Screening of Some Plant Extracts for Their Potential Anticancer Activity
African Journal of Traditional, Complementary and Alternative Medicines
Background: Natural products have been shown to be reliable sources of anticancer medicines although there is still a consistent demand for new therapeutic natural products for cancer treatment with minimal side-effects. Materials and Methods: In this study, six plant extracts (Grevillea robusta; Euphorbia millii; Euphorbia royleana; Aloe grandidentata; Bauhinia corniculate; and Cassia fistula) were screened for the presence of phytochemical metabolites as saponins, tannins, cardiac glycosides, alkaloids, flavonoids, anthraquinones and sterols, using qualitative tests. Antiproliferative screening assay was performed on a panel of three cancer cell-lines (HepG-2, HCT-116 and MCF-7) using MTT assay, and cytotoxicity was determined using WI-38 human fibroblast cell-line. Results: Some plant extracts reduced cellular growth for the selected cancerous cell-lines. For example, E. royleana and A. grandidentata extracts reduced HepG-2 cellular growth with IC50 of 0.42 and 0.53µg/mL, respectively. Moreover, A. grandidentata and C. fistula reduced cellular growth of MCF-7 with IC50 of 0.37 and 0.67 µg/mL, respectively. Conclusion: E. royleana, A. grandidentata and C. fistula showed significant anti-proliferative activity against HepG-2 and MCF-7 cell-lines with non-cytotoxic nature. This suggests their potential role as anticancer agents against these types of cancer. The presence of flavonoids, sterols and anthraquinones may suggest their enhanced anti-proliferative activities. Therefore, this study has shed light on the possible use of these extracts as potential sources of natural products-based therapy for cancer.
2016
The methanolic extracts from ten medicinal plants (Alstonia scholaris, Azadirachta indica, Calotropis gigantea, Emblica officinalis, Mentha citrate, Mentha piperita, Musa paradisiaca, Olea europaea, Punica granatum and Trachyspermum ammi selected from Jammu region, Jammu & Kashmir, India, were evaluated against five human cancer cell lines from four different origins, viz., A-549 (lung), HCT-116 (colon), MCF-7 (breast), PC-3 (prostate) and T-47D (breast) at the concentration of 100 μg/mL using sulphorhodamine blue (SRB) assay. A. indica, O. europaea, M. piperita and M. paradisiaca showed in vitro cytotoxic effect against one or the other human cancer cell line. The methanolic extract of stem and leaves of C. gigantea showed significant cytotoxic activity against four human cancer cell lines from four different tissues. The methanolic extract showed 82-90% growth inhibition at 50 μg/mL and 73-78% growth inhibition at 10 μg/mL against lung, colon and prostate cancer cells. However, 1 ...
Evaluation of some plant extracts for standardization and anticancer activity
Indian journal of traditional knowledge
In recent times, the trend in cancer research is shifting towards identifying new medicines from natural resources for management of cancer. Medicinal plants such as Sthauneyaka (Taxus baccata L.) and compound formulations like Triphala ghrita, Khadirarista, Madhusnuhi rasayana, Maha triphaladya ghrita, Panchatikta guggulu ghrita are indicated in the Ayurvedic texts for management of cancer/ tumour. The anti-proliferative activities of hydro-alcoholic extracts of some standardized plant materials were screened against a panel of 14 human cancer cell lines representing different tissues (lung, pancreas, colon, cervix, oral, bladder, prostate, breast, leukaemia, etc.) through Sulforhodamine-B (SRB) assay. The findings revealed that Cedrus deodara (Roxb.) ex Lamb. and Berberis aristata (Roxb.) ex DC. have maximum anticancer activity against 3 cell lines while Withania somnifera Dunal. showed activity against two cell lines. In addition to these, Picrorhiza kurroa Royle ex Benth. and Pi...
Study of Herbal Anti-Cancer Agents
Journal of emerging technologies and innovative research, 2021
Nowadays, cancer is one of the deadliest diseases in the world, which has been estimated to cause 9.9 million deaths in 2020. Conventional treatments for cancer commonly involve monochemotherapy or a combination of radiotherapy and mono-chemotherapy. However, the negative side effects of these approaches have been extensively reported and have prompted the search of new therapeutic drugs. In this context, scientific community started to look for innovative sources of anticancer compounds in natural sources, including traditional plants. Currently, numerous studies have evaluated the anticancer properties of natural compounds derived from plants, both in vitro and in vivo. In pre-clinical stages, some promising compounds could be mentioned, such as the sulforaphane or different phenolic compounds. On the other hand, some phytochemicals obtained positive results in clinical stages and were further approved for cancer treatment, such as vinca alkaloids or the paclitaxel. Nevertheless, these compounds are not exempt of limitations, such as low solubility, restricted effect on their own, negative side-effects, etc. This review aims to compile the information about the current phytochemicals used for cancer treatment and also promising candidates, main action mechanisms and also reported limitations. In this sense, some strategies to face the limitations have been considered, such as nano-based formulations to improve solubility or chemical modification to reduce toxicity. In conclusion, although more research is still necessary to develop more efficient and safe phytochemical drugs, more of these compounds might be used in future cancer therapies.
Contribution of Selected Medicinal Plants for Cancer Prevention and Therapy
Acta Facultatis Medicae Naissensis, 2000
Since time immortal, plants have been used for maintaining health and curing disease. With cancer being a widespread threat to humanity, plants play an important role in cancer prevention, as well as in therapy. Medicinal plants provide new active chemopreventive molecules. In addition, treatment with plants can ease side-effects as well as provide support to the fears and anxieties of the sick. In this review, methods of exploring new plants and new active plant-derived compounds are described, including ethnobotanical research and screening procedures. Three newly researched medicinal plants, native of Israel, are selected, and new research findings related to their anticancer activities are presented. The plants are: Crocus sativus, Vitex agnus-cactus and Withania somnifera. All three plants are known in traditional medicine and their therapeutical uses are documented. Most findings are preliminary and further studies are required for clinical applications.
Tropical Journal of Pharmaceutical Research, 2015
Purpose: To determine the phenolic composition and antiproliferative activity of 16 different extracts (hexane, dichloromethane, methanol and water) obtained from Bellis perennis, Convolvulus galaticus, Trifolium pannonicum and Lysimachia vulgaris on human breast cancer (MCF-7) and human hepatocellular carcinoma (HepG2/C3A) cell lines. Methods: The aerial parts of the plants were successively extracted with hexane, dichloromethane, methanol and water using a Soxhlet apparatus. The phenolic content of the plants were determined by plants by high performance liquid chromatography (HPLC) while their antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, a yellow tetrazole (MTT) assay. Results: Among the tested extracts, the methanol extract of B. perennis showed the best antiproliferative activity against MCF-7 cell line with IC50 (inhibiting 50 % of cell growth) value of 71.6 µg/mL. Furthermore, the dichloromethane extract of C. galaticus showed the best anti-proliferative activity against HepG2/C3A cell line with IC50 of 57.3 µg/mL. The HPLC data for the plant extracts showed the presence of the following phenolic compounds: gallic acid monohydrate, caffeic acid, rutin hydrate, luteolin-7-O-β-D glucoside, kaempferol, myricetin, quercetin, coumarin and apigenin. Conclusion: The findings of this study indicate that there is some justification for the use of B. perennis and C. galaticus as traditional anticancer medicinal herbs.
2013
Breast cancer is a significant cause of death among women worldwide. Medicinal plant extracts with large amounts of polyphenolic compounds, which are antioxidants, can have an important role in cancer prevention. The purpose of this study is to test in vitro the antiproliferative activities of the ethanolic and aqueous extracts of selected Chinese medicinal plants against three human breast cancer cell lines [MCF-7 (luminal A); HCC1954 (HER2+); Hs578t (triple negative)] and one normal cell line [MCF-10A]. The ethanolic extracts of Polygonum cuspidatum, Rheum officinale, Eucomia ulmoides, Lysinachia christinae, Rhizoma Alpinae, Sarcandra glabre, Scutellaria baicalensis, Smilax glabra and Atractyloides macrocephala showed potent anti-cancer activity with little or no toxicity to normal cells. The apoptosis test was carried out for one of the active plant extract (S. baicalensis) and the results were found to be very significant. The plant extracts investigated in this study have significant anticancer activity against the breast cancer cell lines tested. Further investigation is required to isolate and elucidate the structure of the compounds responsible for the observed activity.