Polymorphism of the ovine calpastatin gene in some Turkish sheep breeds (original) (raw)

2. Materials and methods 2.1. Blood samples Blood samples were randomly collected from 720 lambs born in 2013 belonging to 4 different sheep breeds (GA, KIV, KM, and SZ) reared in West Anatolia. Approximately 4.5 mL blood samples were gathered from the vena jugularis in K3-EDTA tubes and transferred to a-20 ºC freezer. The location and sample sizes of these 4 breeds are given in Table 1. 2.2. DNA isolation The DNA was isolated with a DNA isolation kit (Applied Biological Materials Column-Pure Blood Genomic DNA Kit, Canada) from blood samples as per the manufacturer's instructions in the Adnan Menderes