Sono-Biosynthesis and Characterization of AuNPs from Danube Delta Nymphaea alba Root Extracts and Their Biological Properties (original) (raw)
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Recently, the biosynthesis of gold nanoparticles (AuNPs) has been widely studied and described. In the age of bacterial drug resistance, an intensive search for new agents with antibacterial properties or a new form of antibiotics with effective action is necessary. As a result, the antibacterial activity of AuNPs functionalized with natural compounds is being investigated more frequently. AuNPs biosynthesized with plant extract or functionalized with bioactive compounds isolated from plants could be particularly useful for pharmaceutical applications. The biosynthesized AuNPs are stabilized by an envelope, which may consist of flavonoids, phenolic acids, lipids and proteins as well as carbohydrates and vitamins. The composition of the natural coating affects the size, shape and stability of the AuNPs and is also responsible for interactions with the bacterial cell wall. Recently, several mechanisms of AuNP interactions with bacterial cells have been identified. Nevertheless, they a...
International Journal of Food Science and Technology , 2023
Gold nanoparticles (AuNPs) have been applied in a variety of fields based on their unique physical and chemical properties. Phenolic compounds (PCs) are the largest variety of phytochemicals in foods and have received substantial attention owing to their extraordinary health benefits. Recently, extensive studies have been conducted on the synthesis and conjugation of PCs onto AuNPs (AuNP/PC) characterisation, their bioactivities such as antimicrobial, anticancerous, anti-inflammatory. PCs served the dual roles, i.e., as reductant and stabiliser during the synthesis of AuNPs/PC in a one-pot process. The synthesis parameters for AuNP/PCs and the characteristic properties of as-synthesised AuNP/PCs should be evaluated to achieve the highly efficient bioactivities. Therefore, the critical criteria for the synthesis of AuNP/PC such as the temperature, pH, and molar ratio of the precursors are reviewed. It is found that the PCs mediated reduction of gold salt can provide the well dispersed and stable AuNPs with a high negative surface charge (−15 to −54 mV) and an average SPR band ranging from 520 to 600 nm. The presence of surface hydroxyl groups in AuNP/PCs was the indication of successful conjugation of PCs with AuNPs. It can be concluded that the synergistic effect of the PCs-conjugated AuNPs greatly enhanced their bioactivities.
Journal of Biomedical Materials Research Part A, 2018
In this work, biosynthesis potentials of Dracocephalum kotschyi leaf extract for the production of gold nanoparticle (AuNPs) were studied, and the biological (catalytic, antibacterial, antioxidant and anticancer) activities of studied AuNPs were evaluated. Different analytical techniques including UV-vis spectroscopy, X-ray diffraction (XRD), fourier transform-Infrared spectroscopy (FT-IR), scanning electron microscope (SEM), energy dispersive X-ray analysis (EDX) and transmission electron microscopy (TEM) were used for the characterization of AuNPs. Moreover, Different testing methods were used for evaluating biological activities of biosynthesized AuNPs. The formation of AuNPs was confirmed by color change and UV-visible spectroscopic analysis. FE-SEM and TEM images were used to characterize phytosynthesized AuNPs which were predominantly spherical in shape with size in the range of 5-21 nm. These spherical NPs were found to be 39.79 ±5 nm in size as determined by DLS particle size analyzer. XRD pattern confirms the crystalline nature of the biosynthesized nanoparticles. The phytoconstituents involved in the reduction and stabilization of nanoparticles have been identified using FTIR spectra. The Phytosynthesized AuNPs showed effective antioxidant, antibacterial and catalytic reduction activities. Furthermore, they have inhibited H1229 and MCF-7 cancer cell lines proliferation in a dose-dependent manner. These results have supported that D. kotschyi leaf extract was very efficient for the synthesis of AuNPs, and synthesized NPs showed enhanced biological activities which make them suitable for biomedical applications.
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The current work’s main objective was to determine the chemical composition of Amygdalus communis (AC) leaf extract and examine the antibacterial and cytotoxic properties of biosynthesized gold nanoparticles (AuNPs). The chemical composition of AC leaf extract was determined using LC-ESI/MS/MS to detect compounds that may be responsible for the reducing, stabilizing, and capping steps in the synthesis of nanoparticles and their biological activities. The AC-AuNPs were spherical, with a particle size lower than 100 nm and a face-centered cubic structure. The EDX spectrum confirmed the formation of AuNPs and a negative zeta potential value (−27.7 mV) suggested their physicochemical stability. The in vitro cytotoxic efficacy of the AC-AuNPs against colorectal adenocarcinoma (Caco-2), glioma (U118), and ovarian (Skov-3) cancer cell lines and human dermal fibroblasts (HDFs) was evaluated by MTT assay. CaCo-2 cell proliferation was effectively inhibited by the AC-AuNPs at concentrations b...
International Nano Letters, 2020
Gold nanoparticles (AuNPs) are one of the prominent metal nanoparticles (NPs) with a broad range of applications in vari- ous felds of science and technology. The demand for the use of AuNPs has been increasing on a regular basis. To meet such a demand, intensive research is required on optimizing synthesis approaches. Among various methods reported for the synthesis of AuNPs, biological methods using plant extract have been found to be simple, efective, cheap and environmental friendly because the gold salt is only reduced with biomolecules existing in plant extract. Various methods of AuNPs synthe- sis using diferent plant extracts have long been developed. Several techniques have been adopted in the characterization of the synthesized AuNPs. The antibacterial, antioxidant, anticancer and catalytic activities of AuNPs from plant extract have shown promising activities useful for several biomedical applications. This review focuses on the synthesis, optimization conditions, characterization and applications of biosynthesized AuNPs using plant extracts.
Plants
Rauwolfia serpentina (R. serpentina), belonging to the family Apocynaceae, is a renowned medicinal herb for its different pharmacological activities such as antibacterial, antifungal, anti-inflammatory, and antiproliferative characteristics. This study has done a comparative assessment of the antibacterial, antioxidant, and anti-cancer activity of R. serpentina aqueous leaf extract (RSALE) with encapsulated gold nanoparticles (R-AuNPs). The R-AuNPs are prepared so that they are significant in size, monodispersed, and extremely stable. Their characterization was done by numerous parameters, including UV-visible spectroscopy (528 nm), transmission electron microscopy (~17 d. nm), dynamic light scattering (~68 d. nm), and zeta-potential (~−17 mV). Subsequently, a potent antibacterial activity was depicted via RSALE and R-AuNPs when examined by disc diffusion against various Gram-positive and Gram-negative bacterial strains. The obtained zones of inhibition of RSALE (100 mg/mL) were 34 ...
Arabian Journal of Chemistry, 2018
The aim of this study was to evaluate gold nanoparticles (AuNPs) for their anticancer activity against MCF-7 cancer cells, antioxidant activities and catalysis of the reduction of p-nitrophenol (p-NP). AuNPs were synthesized using kaempferol 3-O-β-Dapiofuranosyl-7-O-α-L-rhamnopyranoside (KG) from the plant Lotus leguminosae. The structure was determined using nuclear magnetic resonance (NMR) and electrospray (ES)-HRMS. The isolated compound was involved in the synthesis and stabilization of AuNPs. A number of parameters such as the pH and the mass ratio (HAuCl 4 / KG) have been optimized to produce very stable colloids of an almost spherical shaped AuNPs with an average diameter of about 37 nm. UV-Vis spectrophotometry, infrared (IR) spectroscopy, transmission electron microscopy (TEM), and thermogravimetric analysis (TGA) studies were employed to characterize the produced nanoparticles. In vitro anticancer studies were performed to assess the chemotherapeutic potential of formulating NPs against a human breast carcinoma cell line (MCF-7). It was observed that the synthesized AuNPs have mild to low cytotoxicity in MCF-7 cells at higher 2 concentrations. The microscopic observations showed no significant changes in the morphology of control cells or the treated cells. AuNPs also displayed strong DPPH radical scavenging compared to the flavonoid extract, with an IC 50 of 30.56 μg/mL. In addition, the biosynthesized AuNPs showed a highly improved catalytic activity for the reduction of p-nitrophenol (p-NP) to p-aminophenol (p-AP), indicative of its potential application in the chemical industry.
Materials Letters, 2019
Synthesis of nanostructures using green chemistry has been popular owing to the exciting properties and innovative applications of these nanostructures. Novel, cost-effective, and eco-friendly gold nanoparticles (Au NPs) were synthesized using a mix of Olea europaea (OE) fruit extract and Acacia nilotica (AN) husk extract. The synthesized Au NPs were characterized by UV-visible spectroscopy, and the average NP size (44.96 nm) was measured by a Zetasizer. A more detailed characterization was performed using Fourier transform infrared spectroscopy, scanning, and transmission electron microscopy and electron microscopy with energy dispersive spectroscopy. The cytotoxicity of the synthesized OEAN Au NPs was evaluated using an MTT assay on breast (MCF-7), colon (TCT-116), and hepatocellular (HCepG-2) carcinoma cells. The antibacterial activity of the synthesized OEAN Au NPs was investigated using a disc diffusion method. The synthesized Au NPs exhibited moderate antibacterial activity against the bacterial strains used and profound anticancer activity against different cell lines used.
Journal of the Taiwan Institute of Chemical Engineers, 2018
A simple, affordable and ecofriendly method for synthesis gold nanoparticles (Au NPs) using Pistacia Atlantica (leave and fruit) extract at the room temperature, has been carry out in the present study. Higher reaction rate about a minute is so noticeable and Au NPs synthesis with this method provided nontoxic carrier for application. Various analytical technique including UV-vis absorption spectroscopy approved presence of Au NPs in the solution, the functional groups of Pistacia Atlantica extract in the reduction and capping process of Au NPs is determined by FT-IR, crystallinity with the fcc plane approved from the X-ray diffraction (XRD) pattern, energy dispersive spectroscopy (EDS) determined existence of elements in the sample, surface morphology, diverse shapes and size of present Au NPs were showed by using scanning electron microscopy (SEM), atomic force microscopy (AFM) and Transmission electron microscopy (TEM). In addition, antibacterial, antioxidant and cytotoxicity properties of Au NPs were studied. Agar disk and agar well diffusion are the methods to determined antibacterial properties of synthesized Au NPs. Also MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) were recognized by macro broth dilution assay. DPPH free radical scavenging assay was used for antioxidant property and compare to butylated hydroxytoluene (BHT) as standard antioxidant. Synthesized Au NPs have great cell viability in a dose depended manner and demonstrate that this method for synthesis Gold nanoparticles provided nontoxic. The average diameter of synthesized Au NPs was about 50-60 nm.