MOESM2 of Ataxia telangiectasia mutated (ATM) interacts with p400 ATPase for an efficient DNA damage response (original) (raw)

2016

Abstract

Additional file 2: Figure S2. (A) ectopic expression of ATM-interacting p400 fragments in U2OS cells. U2OS cells were infected with lentivirus expressing Flag-tagged p400 fragments and selected for 5 days against puromycin. Total 500 μg of protein was immunoprecipitated with M2 agarose and analysed by immunoblotting with anti-Flag antibody. (B) growth curve of puromycin-selected U2OS cells. U2OS cells were infected with lentivirus expressing Flag-F1or Flag-F6, and selected for 5 days against puromycin. Each cell line was seeded in 96-well plate in triplicate and monitored for the cell proliferation.

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