Linking dephosphorylation to cellular events: Functional analysis of human CDC14 (hCDC14) phosphatases (original) (raw)
Mitotic phosphatases play crucial roles in anaphase regulation and mitotic exit by annulling the kinase-mediated protein phosphorylation. In budding yeast Saccharomyces cerevisiae, Cdc14 (cell division cycle 14) phosphatase antagonizes key Cdk1 (cyclin-dependent kinase 1) functions to drive cells out of mitosis. Despite the presence of highly conserved catalytic domains, human CDC14s are dispensable for cell cycle progression. Nevertheless, to decipher the molecular mechanisms of human CDC14s functions, we have investigated the knockout cellular models aided by various imaging and proteomics approaches. Phenotypic analyses of the generated hCDC14A knockout in human retinal pigment epithelium (hTERT-RPE1) cells have exhibited the occurrence of longer primary cilia upon serum starvation. The intermediate longer cilia in haploid-insufficient cells, as well as the extended cilia observed upon siRNA-mediated acute depletion of hCDC14A, have further confirmed the phenotype. Primary cilia ...
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