Structural homologues of CC-NBS-LRR genes for potato late blight resistance in wild Solanum species [Conference poster] (original) (raw)
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Plant Genetic Resources, 2011
New races ofPhytophthora infestansrapidly defeat potato late blight (LB) resistance based onSolanum demissumgermplasm, and breeders search for new sources of durable LB resistance. We developed and verified six sequence characterized amplified region markers recognizing the race-specific genesR1andR3ofS. demissumand the broad-spectrum resistance geneRBofS. bulbocastanumand the germplasms of these species and used them to screen 209 accessions of 21 wildSolanumspecies. In addition toS.demissum, homologues ofR1andR3were found in several species of seriesDemissa,Longipedicellataand diploidTuberosa;R3homologues were also detected inS. bulbocastanum,S. cardiophyllumandS. ehrenbergii.TheRBhomologues were found in a wider range ofSolanumspecies. The markers ofR1andR3genes reliably discerned between germplasms ofS. tuberosumssp.tuberosumand wild sources of LB resistance. Following introgression, the species-specific markers ofdemissumandbulbocastanumgermplasm were rapidly lost, whereas the ...
Solanum bulbocastanum comprising a CC-NBS-LRR gene RB/Rpi-blb1 confers broad-spectrum resistance to Phytophthora infestans and is currently employed in potato breeding for durable late blight (LB) resistance. Genomes of several Solanum species were reported to contain RB homologues with confirmed broad-spectrum defence function. With the discovery that novel P. infestans races break LB resistance mediated by the genes of broad-spectrum specificity, pyramiding several RBlike genes from various Solanum species in a single potato cultivar seems a promising approach to durable LB resistance. Here we report early evidence on RB-like sequences in the wide range of Solanum species section Petota. The panel of Solanum species was screened with three RBrelated PCR markers. RB-like sequences were found in every tested Solanum accession suggesting universal distribution of RB structural homologues among Solanum genomes, while the marker RB-629 corresponding to the RB gene was found in 14 species. The phylogenetic analysis of RB-629 sequences suggested highly conserved pattern of polymorphisms that was neither species-nor seriesspecific. Apparently, duplication and evolution of RB-like loci preceded Solanum speciation. Marker presence and particular haplotypes were not immediately associated with high LB resistance.
Molecular Plant-Microbe Interactions, 2005
Late blight, caused by Phytophthora infestans, is one of the most devastating diseases in cultivated potato. Breeding of new potato cultivars with high levels of resistance to P. infestans is considered the most durable strategy for future potato cultivation. In this study, we report the identification of a new late-blight resistance (R) locus from the wild potato species Solanum bulbocastanum. Using several different approaches, a high-resolution genetic map of the new locus was generated, delimiting Rpi-blb3 to a 0.93 cM interval on chromosome 4. One amplification fragment length polymorphism marker was identified that cosegregated in 1,396 progeny plants of an intraspecific mapping population with Rpi-blb3. For comparative genomics purposes, markers linked to Rpi-blb3 were tested in mapping populations used to map the three other late-blight R loci Rpi-abpt, R2, and R2-like also to chromosome 4. Marker order and allelic conservation suggest that Rpi-blb3, Rpiabpt, R2, and R2-like...
Plant Genetic Resources, 2011
A coiled coil-nucleotide binding site-leucine-rich repeat geneRB/Rpi-blb1isolated fromSolanum bulbocastanumconfers broad-spectrum resistance toPhytophthora infestansand is currently employed in potato breeding for durable late blight (LB) resistance.RBhomologues were reported in severalSolanumspecies; some of them retained defence function. Here, we report additional evidence onRB-like sequences in 21Solanumspecies of the sectionPetota. The panel ofSolanumspecies was screened with threeRB-related PCR markers.RB-like sequences were found in every testedSolanumaccession, suggesting universal distribution ofRBstructural homologues amongSolanumgenomes, while locus-specific RB-629 was found only in 15 species. Phylogenetic analysis of RB-629 sequences suggested a highly conservative pattern of polymorphisms that was neither species- nor series-specific. Apparently, duplication and evolution ofRB-like loci precededSolanumspeciation. Marker presence and particular haplotypes were not immed...
Proceedings of the 12th Euroblight Workshop, PPO-Special Report no. 14 (2010), 231- 238, 2010
New races of Phytophthora infestans are known to rapidly defeat potato late blight (LB) resistance introgressed with the germplasm of Solanum demissum, the early source of race-specific resistance genes (R-genes). Nonetheless, the presence of the major demissum gene R1 in potato cultivars was associated with higher field indices of LB resistance (Stewart et al., 2003; Gebhardt et al., 2004; Beketova et al., 2006) suggesting that these demissum R-genes in some way add to plant defense response. We developed and verified SCAR markers recognizing the race-specific genes R1 and R3 of S. demissum and S. stoloniferum and the germplasms of these species. By screening wild Solanum species and potato accessions reportedly free from wild Solanum germplasm (Chilotanum varieties and old cultivars), we established that these markers reliably discerned between germplasms of cultivated Solanum tuberosum ssp. tuberosum and wild sources of LB resistance. Screening 161 potato cultivars demonstrated that the presence of SCAR markers of R1 and R3 genes was significantly related to higher indices of LB resistance. Similar results were obtained when the presence of demissum R-genes was recognized using specific races of Ph. infestans. Such association presumes that both R1 and R3 genes contribute to overall LB resistance of potato cultivars.
2010
Solanum bulbocastanum comprising a CC-NBS-LRR gene RB/Rpi-blb1 confers broad-spectrum resistance to Phytophthora infestans and is currently employed in potato breeding for durable late blight (LB) resistance. Genomes of several Solanum species were reported to contain RB homologues with confirmed broad-spectrum defence function. With the discovery that novel P. infestans races break LB resistance mediated by the genes of broad-spectrum specificity, pyramiding several RBlike genes from various Solanum species in a single potato cultivar seems a promising approach to durable LB resistance. Here we report early evidence on RB-like sequences in the wide range of Solanum species section Petota. The panel of Solanum species was screened with three RBrelated PCR markers. RB-like sequences were found in every tested Solanum accession suggesting universal distribution of RB structural homologues among Solanum genomes, while the marker RB-629 corresponding to the RB gene was found in 14 speci...
Theoretical and Applied Genetics, 2005
The cultivated potato, Solanum tuberosum, is affected by a variety of diseases with late blight, caused by Phytophthora infestans, being the most severe. Wild potato species have proven to be a continuing source of resistance, sometimes of an extreme type, to this disease. The present study constructs the first late blight linkage map of a member of series Piurana, S. paucissectum, a tuber-bearing relative of potato, using probes for conserved sequences from potato and tomato. Eight probes mapped to unexpected linkage groups, but syntenic differences with prior maps of potato were not supported by any blocks of rearranged chromosome segments. All 12 linkage groups were resolved and significant associations with late blight resistance were found on chromosomes 10, 11 and 12. A major quantitative trait locus (QTL) on chromosome 11 accounts for more than 25% of the phenotypic variance measured in a field trial. Crossing of S. paucissectum with cultivated potato resulted in very few seeds indicating partial reproductive barriers. Differential reactions of accessions of this potential donor species with simple and complex isolates of P. infestans suggest that it carries major resistance genes that are not those previously described from the Mexican species, S. demissum. However, the additivity of the QTL effects argues for the quantitative nature of resistance in this cross.
2011
The panel of wild Solanum species and potato hybrid clones was studied by coupled phytopathological and SCAR-marker analyses. Late blight (LB) resistance was assessed in individual plants under the field conditions and in the laboratory, with the detached-leaf test. The novel lines derived from previously unexplored sources of LB resistance among the Bolivian diploid species S. alandiae manifested a successful field resistance in the trials through two epidemic years. SCAR markers were developed using the R1, R3a and RB gene sequences. By screening wild Solanum species and clones of potato hybrids after natural and artificial infestation by P. infestans, we established that the presence of SCAR markers R1-1205 was significantly related to LB resistance. This evidence suggests the practicability of employing this marker as a breeding tool for early prediction of LB resistance in a wide range of Solanum germplasms.
Molecular Plant-Microbe Interactions, 2009
Late blight, caused by the oomycete Phytophthora infestans, is one of the most devastating diseases of potato. Resistance (R) genes from the wild species Solanum demissum have been used by breeders to generate late-blight-resistant cultivars but resistance was soon overcome by the pathogen. A more recent screening of a large number of wild species has led to the identification of novel sources of resistance, many of which are currently being characterized further. Here, we report on the cloning of dominant Rpi genes from S. venturii. Rpi-vnt1.1 and Rpi-vnt1.3 were mapped to chromosome 9 using nucleotide binding site (NBS) profiling. Subsequently, a Tm-22-based allele mining strategy was used to clone both genes. Rpi-vnt1.1 and Rpi-vnt1.3 belong to the coiled-coil NBS leucine-rich repeat (LRR) class of plant R genes and encode predicted peptides of 891 and 905 amino acids (aa), respectively, which share 75% amino acid identity with the Tomato mosaic virus resistance protein Tm-22 fro...
Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato
Plant Journal, 2005
Comparative genomics provides a tool to utilize the exponentially increasing sequence information from model plants to clone agronomically important genes from less studied crop species. Plant disease resistance (R) loci frequently lack synteny between related species of cereals and crucifers but appear to be positionally well conserved in the Solanaceae. In this report, we adopted a local RGA approach using genomic information from the model Solanaceous plant tomato to isolate R3a, a potato gene that confers race-specific resistance to the late blight pathogen Phytophthora infestans. R3a is a member of the R3 complex locus on chromosome 11. Comparative analyses of the R3 complex locus with the corresponding I2 complex locus in tomato suggest that this is an ancient locus involved in plant innate immunity against oomycete and fungal pathogens. However, the R3 complex locus has evolved after divergence from tomato and the locus has experienced a significant expansion in potato without disruption of the flanking colinearity. This expansion has resulted in an increase in the number of R genes and in functional diversification, which has probably been driven by the co-evolutionary history between P. infestans and its host potato. Constitutive expression was observed for the R3a gene, as well as some of its paralogues whose functions remain unknown.