Conformational studies of a hyperthermostable enzyme (original) (raw)

AI-generated Abstract

Conformational studies of a hyperthermostable enzyme, specifically the extracellular endo-1,3-glucanase (LamA) from Pyrococcus furiosus, reveal insights into its structural characteristics at extreme temperatures and in high concentrations of guanidine hydrochloride. Utilizing circular dichroism, steady-state, and time-resolved fluorescence spectroscopy, the research identifies distinct conformational states of LamA, including native, partially folded, and completely unfolded forms. The findings indicate the enzyme retains structural integrity at high temperatures, with significant denaturation occurring only at 150°C, emphasizing its potential applications in biotechnology.