Sampling strategies for characterization of neuropeptides using mass spectrometry and functional implications into cell-cell signaling (original) (raw)

In this dissertation, there are developed different analytical strategies to discover and characterize mammalian brain peptides using small amount of tissues. The magnocellular neurons of rat supraoptic nucleus in tissue and cell culture served as the main model to study neuropeptides, in addition to hippocampal neurons and mouse embryonic pituitaries. The neuropeptidomcis studies dcscribed here usc differcnt extraction methods on tissue or cell culture combined with mass spectrometry (MS) techniques, matrix-assisted laser desorptionlionization (MALDI) and eleetrospray ionization (ESl). Thesc strategies lead to the identification of multiple peptides from the rat/mouse brain in tissue and cell cultures, including novel compounds One of the goals in this dissertation was to optimize sample preparations on samples isolated from well-defined brain regions for mass spectromctric analysis. Here, the neuropeptidomics study of the SON resulted in the identification of 85 peptides, includin...