Medroxyprogesterone Acetate Reduced Cellular Proliferation of the Luminal and Glandular Epithelium in the Developing Canine Uterus (original) (raw)

The uterine gland knockout ( U G K O ) p h e n o t y p e w a s produced in both sheep and mice by strategic administration of progestins to neonates from birth (Postnatal Day = PND 0). Adult UGKO animals lack uterine g lands and cannot suppor t pregnancy. Induction of the UGKO phenotype in dogs would provide a means of inducing sterility non-surgically. In the dog, uterine gland development begins during the first week of neonatal life and progesterone receptors are present in uterine tissue at this time. The objectives of this study were to determine the effects of neonatal progestin treatment on canine uterine g land deve lopment . Seven mixed breed puppies were given either medroxyprogesterone acetate (MPA; n=3) (10mg/ kg body weight, i .m.) or an equal volume of sterile saline (n=4) at PND 5 and again once birth weight had tripled. Gland penetration measurements were obtained from uterine crosssections which were stained with Hematoxylin and imaged us ing the Aper io ® Imaging s...