Antimicrobial Compound from Streptomyces Isolate Characterized Using HPLC (original) (raw)
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Life Science Journal
In the present study total 43 actinomycetes RMN1-RMN43 were isolated from the collected soil samples. To screen out active metabolites producing actinomycetes against human pathogenic bacteria, 30 Gram positive and negative bacteria were isolated from hospitalized patients. In the primary screening, 21 actinomycetes showed antibacterial potential against one or more pathogenic bacteria. Total 14 actinomycetes, RMN1-14, showed antibacterial activities through agar well diffusion assay. One isolate of actinomycete, RMN5, produced exclusively narrow spectrum bioactive secondary metabolites against E. coli with 13 mm zone of inhibition. In the secondary screening most potent actinomycete, RMN6, produced broad spectrum bioactive metabolites against ATCC bacterial culture and multi antibiotic resistant bacteria such as E. coli ATCC 25922, P. aeroginosa ATCC 27553, and Staphylococci ATCC 25923 activities at the range of 14, 13 and 19 mm zone of inhibition respectively while against isolate...
2021
Realizing an increasing need for a novel antibiotic, this study was carried out to screen antibacterial metabolites producing actinomycetes from 15 soil samples collected from Taplejung. Antibacterial metabolites producing actinomycetes were confirmed by primary screening and secondary screening. Macroscopic, microscopic, and biochemical characteristics were used for presumptive identification of probable actinomycetes genera. The potential isolate was cultured in starch casein broth for production of possible antibacterial compound. The antibacterial compound was extracted from fermented broth using organic solvents like ethyl acetate, n-butanol, chloroform, dichloromethane, and methanol. Among 24 isolates, only one (T18) showed antibacterial activity against both Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli, Salmonella Typhi and Pseudomonas aeruginosa) test-bacteria. The isolate was considered as Streptomyces spp based on microsco...
The purpose of this study was to isolate the antibiotic producing actinomycetes from soil from different parts of Kathmandu valley and to access their antimicrobial activity against different pathogenic bacteria like Staphylococcus aureus (ATCC 25923), Escherichia coli(ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853). This study also focuses on investigating the distribution of the antibiotic producing actinomycetes according to the texture and cultivation status of soil. Fifty four colonies of actinomycetes were isolated from the soil collected from five different parts of Kathmandu valley in the standard medium out of which 20 colonies produced antibiotics; all of them belonged to genus Streptomyces. One of the isolated colony largely inhibited the growth of E. coli and Staphylococcus aureus with zone of inhibition more than 20 mm for both. Antibiotic from this colony was produced by submerged fermentation and the minimum inhibitory concentration of the crude antibiotic was determined.
Actinobacteria have gained prominence in recent years because of their potential for producing antibiotics which are not only used in pharmaceutical industries but also in the agriculture practice to inhibit the growth of several phyto-pathogens. A total of 5 different types of actinobacteria were isolated from the soil of terrestrial environment from Thanjavur District. All the isolated actinobacteria were characterized and identified based on the morphological, biochemical and molecular characteristics. The isolates were belonged to the genera Streptomyces KV1, KV2, KV3, KV4 and KV5. The isolated strains were screened by cross streak and well diffusion methods. Only Streptomyces sp KV2 was selected for further studies. The result of the molecular characteristics selected Streptomyces sp. (KV2) identified as S. champavatii (KV2). The result of the screening revealed that the crude extract was effective against human and plant pathogens. The antimicrobial efficacies and solubility of the antimicrobial compound was evaluated and extracted with organic solvents. Extracted antimicrobial compound of the Streptomyces sp. KV2 was characterized by using UV, FT-IR and 1H and 13C NMR spectral analyses. Rf value in the TLC chromatogram characterized that, the metabolite comes under , -O-H, -C-O-CH3, F-CH3, and group very much related to Staurosporine.
Acquisition of Actinobacteria, especially Streptomyces from previously underexplored habitats and the exploration of their biosynthetic potential have gained much attention in the rejuvenated antibiotics search programs. Herein, we isolated some Streptomyces strains, from an arid region of the Great Indian Thar Desert, which possess an ability to produce novel bioactive compounds. Twenty-one morphologically distinctive strains differing in their aerial and substrate mycelium were isolated by employing a stamping method. Among them, 12 strains were identified by a two-level antimicrobial screening method, exerting antimicrobial effects against a panel of indicator strains including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus species. Based on their potent antimicrobial activity, four isolates were further explored by 16S rRNA gene-based identification, genetic screening, and metabolomic analysis; and it was found that these strains belong to the ...
Antimicrobial activity of Streptomyces spp. ERI26 recovered from Western Ghats of Tamil Nadu
Journal De Mycologie Medicale, 2008
A new actinomycetes isolate ERI-26 was recovered from Nilgiri forest soil of Western Ghats and screened for its antimicrobial activity against pathogenic bacteria and fungi. Cultural characteristics were assessed in different culture media and 16s rRNA pattern strongly suggested that this isolate belonged to Streptomyces. The morphological and physiological characters were recorded. ERI-26 exhibited resistance to streptomycin. ERI-26 had the ability to produce enzymes such as amylase, cellulase and catalase. Maximum biological activity was obtained on Modified Nutrient Glucose Agar (MNGA) medium. ERI-26 showed activity against bacteria such as Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and fungi such as Candida albicans, Aspergillus niger and Aspergillus flavus. The antibacterial substances were extracted using methanol from MNGA medium in which ERI-26 had grown for six days at 28 8C. The antimicrobial activity was assessed using broth microdilution technique. The lowest Minimum Inhibitory Concentration of methanol fractions of ERI-26 against S. epidermidis was 375 mg/ml and against C. albicans was 500 mg/ml. # 2008 Elsevier Masson SAS. All rights reserved.
Journal of Microbial & Biochemical Technology, 2018
The Streptomyces genus is well studied owing to its capacity in producing more than 70% of antibiotics. This study was undertaken to characterize Streptomyces strains, occurring in soils of different districts of Tamilnadu, India as well as to evaluate their potential to produce antimicrobial compounds. Samples were collected from rice rhizosphere from different district of Tamilnadu agricultural zone. In primary screening by cross streak method, Streptomyces strains were assessed for antibiotic production and activity against different human bacterial. Then active isolates were selected for secondary screening by agar well diffusion method. Solvent extraction method was used to identify the best crude samples which are exhibiting better antibacterial activity. Then, 16S rRNA PCR was carried out for confirmation of isolates. In primary screening among all of the isolates, 50% isolates were active against at least one of the test organisms and 21.31% strains exhibited a broadspectrum activity against almost all of the test bacteria. The minimum inhibitory concentrations (MICs) of the ethyl acetate extracts measured. Out of 26 positive strains two of the most active isolates SVG-07-15 and TK-01-05 were taken for further studies.These results highlight the importance of Streptomyces isolates in antibiotic production. Together with antibacterial activity, the PKS gene based approach can be applied for efficient screening of isolated strains of pharmaceutical value and related compounds.
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES
The aim of this study was to isolate actinomycetes strains having antibacterial activity from soil samples collected from various places around Gazipur, Bangladesh. From four soil samples using Starchcasein- nitrate-agar medium, a total of eight actinomycetes strains were isolated in pure form. These strains were primarily screened for their antibacterial activity against a series of test bacteria. Five (62.5%) strains showed moderate to high antibacterial activity. Among them, due to the highest antibacterial activity, visibility of the metabolite as well as excellent growth properties, ASAR-5 strain was selected for morphological, cultural, physiological and biochemical studies and these studies strongly suggested that the isolate belonged to Streptomyces sp. The extract of metabolite from this potential strain was obtained by solvent extraction method using ethyl acetate. The extracts exhibited reasonably strong antibacterial activity against a series of Gram positive and Gram ne...