Diversity among field populations of bacterial strains nodulating lupins in Poland (original) (raw)

2009, Fragmenta Agronomica

235 bacterial isolates were collected from 23 locations of lupine fi eld crops, including 16 geographical locations, mainly from Wielkopolska. Bacteria were isolated from root nodules of yellow lupine (Lupinus luteus), white lupine (Lupinus albus), and blue lupine (Lupinus angustifolius). Observations presented in this paper confi rmed the existence of large genetic diversity within the bacterial population nodulating lupines, but also allowed the separation of groups of bacteria with similar characteristics. It seems that the symbiotic processes involving the participation of lupines and bacteria belonging to at least two types of Rhizobiaceae. Interesting in this context is as the ratio of strains alkalizing substrate (characteristic for Bradyrhizobium) to acidifying strains (which is characteristic for Rhizobium) in the analyzed population. This ratio is 3:7 respectively, suggesting the domination of Rhizobium among lupine microsymbiont population Poland. The large diversity of populations and a wide range of bacteria Rhizobium and Bradyrhizobium confi rmed by the observations of this work indeed appears to show a long history of bacteria belonging to these groups, and that bacterial populations capable of lupine nodulation are indeed endogenous, perfectly suited to local environmental conditions in Polish soils.