Bioprospecting Antimicrobial Potential of Lignin Stream of Paddy Straw Against Food-Borne Pathogens (original) (raw)
Related papers
Antimicrobial and antioxidant activities of lignin from residue of corn stover to ethanol production
Industrial Crops and Products, 2011
To improve the economic viability of the biofuel production from biomass resource, a value-added lignin byproduct from this process is increasingly important. Antioxidant and antimicrobial activities of lignin extracted from residue of corn stover to ethanol production were investigated. The lignin extracts exhibited strong antioxidant activities in hydrophilic oxygen radical absorbance capacity (ORAC) assay and Folin-Ciocalteu test. The extracts also exhibited antimicrobial activities against Gram-positive bacteria (Listeria monocytogenes and Staphylococcus aureus) and yeast (Candida lipolytica), but not Gram-negative bacteria (Escherichia coli O157:H7 and Salmonella Enteritidis) or bacteriophage MS2. Different extraction conditions (temperature and residue/solvent ratio) affected the antioxidant and antimicrobial activities of lignin extracts. Generally, the bioactivities of lignin extracts were consistent with FTIR analysis results. Lignin byproducts showed the potential for their antioxidant and antimicrobial application.
Applied Biochemistry and Biotechnology, 2017
Lignin accounts for 15-35% of dry biomass materials. Therefore, developing valueadded co-products from lignin residues is increasingly important to improve the economic viability of biofuel production from biomass resources. The main objective of this work was to study the lignin extracts from corn stover residue obtained from a new and improved process for bioethanol production. Extraction conditions that favored high lignin yield were optimized, and antioxidant and antimicrobial activities of the resulting lignin were investigated. Potential estrogenic toxicity of lignin extracts was also evaluated. The corn stover was pretreated by low-moisture anhydrous ammonia (LMAA) and then subjected to enzymatic hydrolysis using cellulase and hemicellulase. The residues were then added with sodium hydroxide and extracted for different temperatures and times for enhancing lignin yield and the bioactivities. The optimal extraction conditions using 4% (w/v) sodium hydroxide were determined to be 50°C, 120 min, and 1:8 (w:v), the ratio between corn stover solids and extracting liquid. Under the optimal condition, 33.92 g of lignin yield per 100 g of corn stover residue was obtained.
Polymers
The antiradical and antimicrobial activity of lignin and lignin-based films are both of great interest for applications such as food packaging additives. The polyphenolic structure of lignin in addition to the presence of O-containing functional groups is potentially responsible for these activities. This study used DPPH assays to discuss the antiradical activity of HPMC/lignin and HPMC/lignin/chitosan films. The scavenging activity (SA) of both binary (HPMC/lignin) and ternary (HPMC/lignin/chitosan) systems was affected by the percentage of the added lignin: the 5% addition showed the highest activity and the 30% addition had the lowest. Both scavenging activity and antimicrobial activity are dependent on the biomass source showing the following trend: organosolv of softwood > kraft of softwood > organosolv of grass. Testing the antimicrobial activities of lignins and lignin-containing films showed high antimicrobial activities against Gram-positive and Gram-negative bacteria...
Chemical Modification and Characterization of Straw Lignin
Cellulose Chemistry and Technology, 2009
This work aims at describing the modification and characterization of lignin separated from annual plants (wheat straw) by their delignification through the alkaline method. The lignin has been subjected to the hydroxymethylation reaction, for introducing hydroxyl groups into its structure, thus assuring a more complete exploitation of this natural aromatic polymer. Chemical and spectral analyses (FTIR, UV-VIS, fluorescence, HPLC), and thermal stability characterization (TG, DTG) have been carried out to evidence the transformations occurring in the lignin macromolecule. The experimental data show that the hydroxymethylation reaction induces the modification of lignin functionality and polymolecularity. Further, the properties of modified lignin were demonstrated in experiments on wood bioprotection.
Physico-Chemical and Thermal Characterization of Alkali-Soluble Lignins from Wheat Straw
Polymer Journal, 1998
Four alkali-soluble lignin fractions were extracted from dewaxed wheat straw with 3% sodium hydroxide at 45°C for 2, 5, 12, and 15 h, respectively. The pure alkali-soluble lignin preparations LA were obtained by using a two step precipitation method instead of the traditional ether precipitation procedure. The physico-chemical properties and structural features of the isolated pure lignin fractions were characterized by UV, FT-IR, 13 C NMR spectroscopy, and thermal analysis. The lignin in fraction LA-I is mainly composed of /J-O-4 ether bonds. The less common /J-5 and /J-/J carbon-<:arbon linkages are also present in the lignin structural units. The results obtained showed that the lignin fractions appeared to be strongly associated to hydroxycinnamic acids such as p-coumaric and ferulic acids and glucuronic acid or 4-O-methylglucuronic acid.
Characterization of Lignins Isolated from Industrial Residues and their Beneficial Uses
BioResources, 2016
The physico-chemical properties of lignin isolated from lignocellulosic bioethanol residues and hardwood kraft black liquor were compared with two commercial lignins, kraft softwood lignin, and soda non-wood lignin. Lignin from the industrial residues was isolated through the acid precipitation method. The amount of lignin isolated was approximately 38% of the dry weight of lignocellulosic bioethanol residues and approximately 27% of the black liquor solids. The numbers of methoxyl groups and phenolic and aliphatic hydroxyls were determined to derive a molecular formula for each of the four lignins. The molecular weights of the lignins were measured by high performance size exclusion chromatography. Potential value-added applications of the lignins were summarized based on their molecular weights and physico-chemical characteristics.
Industrial Crops and Products, 2016
The lignin is the most important renewable source of aromatic compounds on earth. It could represent up to 40% of dry matter in a biomass, however, its potential is underestimated being used as heat recovery source in industrial processes. Therefore, this work aimed to show the chemical feature of lignin isolated from sequential acid-alkaline pretreatment of oil palm empty fruit bunches. The extracted lignin was subjected to studies of this thermal behavior and biological properties as antioxidant, antimicrobial, and antidiabetic. The 2D HSQC spectroscopy analysis showed syringyl aromatic structure and presence of aromatic rings in lignin. The antioxidant assay showed that 2 mg of lignin were required to inhibit 50 wt.% DPPH, while the antimicrobial test inhibited the growth of Escherichia coli, Salmonella enterica serovar thyphimurium, Bacillus subtilis and Staphyloccocus aureus. The antidiabetic assay revealed inhibition of 20% of ␣-amylase activity. The thermogravimetric analysis gave out two peaks of decomposition at 230 • C and 350 • C and the glass transition temperature at 70 • C. These results showed the potential of lignin as precursor of chemicals of added value in a biorefinery process using as feedstock oil palm empty fruit bunches.
Non-catalytic green solvent lignin isolation process from wheat straw and the structural analysis
Renewable Energy, 2019
In this study, the optimal experimental conditions for extraction of organosolv lignin from wheat straw were determined. Initially, the wheat straw chemical compositions were investigated. The wheat straw held 24.6 lignin and 60% cellulose and hemicellulose on a dry weight base. The lignin extractions from wheat straw were carried out by applying 60% aqueous ethanol in a 600-mL reactor at different times (60-90-120-150 min), temperatures (100-150-200-250°C) and pressures (300-350-400-450 psi). The extraction was done without the use of any catalysts and all the green solvents were recovered and reused. The highest yield of lignin (90%) was obtained under optimal conditions (120 min, 200°C, and 400 psi). The extracted lignin also contained the low amount of carbohydrate impurities and ash (2.2 %). The results from Size exclusion chromatography and MALDI-TOF suggested that the number of average molecular weight and the molecular weight of extracted lignin are 7870 and 9046 Da. Extracted lignin had Coniferyl, sinapyl and coumaryl alcohols in its structure. The results from ANOVA showed that lignin extraction was mostly influenced by time, and the temperature had the least effect on it. Ultimately, to confirm the sustainability of the process, the extraction was conducted in an 18.75 L reactor.
ACS Sustainable Chemistry & Engineering, 2018
In this study, dissolution of pristine alkali lignin into ethylene glycol, followed by addition of different acidic conditions (HCl, H 2 SO 4 and H 3 PO 4 at different pH) has been considered as a simple method to prepare high yield lignin nanoparticles (LNP). Field emission scanning electron microscopy (FESEM), Nano-Zetzsozer (ZS), gel permeation chromatography (GPC) and thermo gravimetric analysis (TGA) have been utilized to determine the influences of the precipitation procedures on particle size, Zeta potential, molecular weight and thermal stability of final obtained LNP. Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and nuclear magnetic resonance (NMR) were also considered to investigate the influences of lignin chemical structures and composition on its antioxidative and antimicrobial behaviours. Results from DPPH (1,1-Diphenyl-2-picryl-hydrazyl) activity revealed the antioxidant response of LNP aqueous solution, whereas results from antimicrobial tests confirmed LNP as effective antibacterial agents against Gram negative bacteria Pseudomonas syringae pv. tomato (CFBP 1323) (Pst), Xanthomonas axonopodis pv. vesicatoria (CFBP 3274) (Xav) and Xanthomonas arboricola pv. pruni (CFBP 3894) (Xap) plant pathogen strains. The results confirmed how high efficient antioxidant and antimicrobial LNP could be considered as an easy methodology for plant pathogens control.