Candida batistae, a new yeast species associated with solitary digger nesting bees in Brazil (original) (raw)

and , two new yeast species from the clade associated with solitary bees in the Atlantic rain forest of Brazil

FEMS Yeast Research, 2005

Two new ascomycetous yeast species belonging to the Starmerella clade were discovered in nests of two solitary bee species in the Atlantic rain forest of Brazil. Candida riodocensis was isolated from pollen-nectar provisions, larvae and fecal pellets of nests of Megachile sp., and Candida cellae was found in pollen-nectar provisions of Centris tarsata. Analysis of the sequences of the D1/D2 large-subunit ribosomal DNA showed that C. riodocensis is phylogenetically related to C. batistae, and the closest relative of C. cellae is C. etchellsii. The type strains are C. riodocensis UFMG-MG02

Prospection on Yeasts from Stingless Bees Honey in BrazilianTropical Dry Forest (Caatinga)

Gaia Scientia

The richness of the yeasts associated with honey obtained from the stingless bees Melipona mandacaia, M. asilvai, Patarmona sp. and Scaptotrigona sp. living in Brazilian tropical dry forest (Caatinga) was studied. Based on morphological and physiological characters 12 species were identified belonging to the phylum Ascomycota, with eight Candida species. This study has shown that the honey of stingless bees is an important source of yeasts, particularly in tropical dry environments, where the diversity of fungi is still largely unknown.

Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees

Scientific Reports

Stingless bees are a diverse group with a relevant role in pollinating native species. Its diet is rich in carbohydrates and proteins, by collecting pollen and nectar supplies the development of its offspring. Fermentation of these products is associated with microorganisms in the colony. However, the composition of microorganisms that comprise this microbiome and its fundamental role in colony development is still unclear. To characterize the colonizing microorganisms of larval food in the brood cells of stingless bees Frieseomelitta varia, Melipona quadrifasciata, Melipona scutellaris, and Tetragonisca angustula, we have utilized molecular and culture-based techniques. Bacteria of the phyla Firmicutes, Proteobacteria, Actinobacteria, and fungi of the phyla Ascomycota, Basidiomycota, Mucoromycota, and Mortierellomycota were found. Diversity analysis showed that F. varia had a greater diversity of bacteria in its microbiota, and T. angustula had a greater diversity of fungi. The iso...