Osteocalcin expression of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) added with hydroxyapatite (HA) in rabbit’s post extraction tooth sockets (original) (raw)
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Indonesian Journal of Dental Medicine, 2021
Background: Bone graft materials have been used extensively to support bone healing after tooth extraction. Bone healing could be increased reactive oxygen species (ROS) that prolong the phase of the inflammatory and delay reparative phase. Antioxidants are substances that can improve and reduce the number and the damage produced by ROS. Hence, the utilization of antioxidant which is utilized in conjunction to Carbonate hydroxyapatite is expected to increase the success of bone healing. Purpose: The aim of this study is to compare the number of osteoblasts and osteoclast in the process of bone healing after employing Carbonate Hydroxyapatite and antioxidant in the Wistar rats’ incisor tooth extraction socket. Methods: Twenty-seven male Rattus norvegicus strain Wistar rats were divided into 3 treatment groups. This study uses a post-test only control design. Sample of 27 rats were divided into 3 groups. Mandibular incisive is extraction. Group 1, socket is lefted to fill with blood (...
Journal of Oral Biology and Craniofacial Research, 2020
The aim of the study was to evaluate the influence of autologous platelet-rich fibrin (PRF) on soft tissue healing and bone regeneration following tooth extraction clinically and radio-graphically. Materials and methods: 30 Patients between the age group of 18-40 years requiring extraction of bilateral mandibular molars except third molars were selected to conduct a split-mouth study after ethical approval. Teeth extraction was done on both sides in the same appointment. Autologous PRF was placed into the socket on one side randomly, and the socket on the other side was taken as control side. Parameters evaluated were soft tissue healing and bone regeneration. Soft tissue healing was evaluated on post-extraction day-3, day-7 and day-14 using healing index by Landry et al. Bone regeneration was assessed immediately and 4 months post-extraction by observation of change in radiopacity through digital panoramic-radiograph. Data obtained was statistically analysed and comparison of outcome variables was done using Mann-Whitney U-test, p < 0.05 was considered statistically significant. Results: Case group had better soft tissue healing when compared to the control group with the p-value of 0.025 at 3rd day 0.039 at 7th day and 0.00 at 14th day. The rise in radiopacity at the end of 16th week for PRF group was higher as compared to control group but did not differ significantly. Conclusion: PRF is significantly better in promoting soft tissue healing and also hastens bone formation in extraction socket. PRF may be recommended as a valuable material for encouraging soft tissue healing and bone regeneration.
The Assessment of the Usefulness of Platelet-Rich Fibrin in the Healing Process Bone Resorption
Coatings, 2022
The main subject of this research was the use of PRF in dental surgery aimed at preventing changes in alveolar height and width after tooth extraction. Due to the large growth factor content, it seems to be particularly useful in bone loss management starting from the simplest loss occurring after tooth extraction through loss resulting from tooth resection ending with loss caused by large bone cysts. The study was performed on 50 patients. The extraction of two maxillary or mandibular homonymous teeth was carried out in each patient, where PRF was placed in one alveolus while the other alveolus was left empty. Then, the alveoli were surgically managed with a split flap technique. On the extraction day, after 10 days, and after 6 months, the alveolar process was measured, soft tissues healing was assessed, and imaging examinations were analyzed. It was proved that the healing of soft tissues in the PRF group was better. In the PRF group after 6 months from surgery, the newly formed ...
From Coagulation to Oral Surgery Application: Platelets in Bone Regeneration
2018
The complexity of the treatment of tissue lesions, particularly bone lesions, in regenerative medicine depends on the origin of the substance loss (traumatic, tumoral, infectious, etc.), its size and mechanical requirements. In the field of dental surgery, the need to ensure rapid regeneration of injured bone tissue for periodontal, post-extractional or pre-implant corrective surgery leads dental surgeons to have a large number of biomaterials in their therapeutic arsenal. The mineral materials are most often used because of their chemical composition which is close to bone’s mineral phase. They also present a resorption time in agreement with the time of formation of new bone.However their benefits are inconstant and the need of new bioactive structures, well accepted by the host, and favoring tissue healing has grown. Here is the place for platelet concentrates such as Platelet Rich Plasma (PRP) and Platelet Rich Fibrin (PRF) which are rich in growth factors, cytokines and others ...
International journal of health sciences
Purpose: The present study was performed to evaluate clinically, radiographically and histologically the effect of autologous platelet rich fibrin (PRF) and 1% alendronate (ALN) gel in alveolar ridge preservation after tooth extraction. Materials and method: In this study, thirty patients with teeth indicated for extraction were divided randomly into three groups: Group A: the patients received 1% ALN gel mixed with PRF as a grafting material for the extraction socket. Group B: the patients received PRF only and in Group C the socket left without any a grafting material. All patients were evaluated clinically and radiographically at; base line and after 3 months. Also, histological evaluation using bone core biopsy harvested at the re-entry surgery before implant placement. Results of the present study were recorded, tabulated and statistically analyzed. Results: Histological, clinical and radiographic findings showed a statistically significant difference in group A when compared t...
Anatomical Science International, 2019
Several methods have been developed to regenerate lost alveolar bone. Platelet-rich fibrin (PRF) is a useful adjunct for new bone formation in dentistry. To elucidate the effect of advanced PRF (A-PRF) on bone formation, we inserted A-PRF clots in sockets after tooth extraction. Premolars were extracted from beagle dogs, and A-PRF was applied to the socket. New bone formation was assessed using histological and immunofluorescence examinations, and the bone formation ratio was evaluated 14 and 30 days postoperatively. Histological examination revealed newly formed bone filling the sockets up to the center in the A-PRF group at 14 days postoperatively, while thick and regular bone trabeculae were arranged in porous bone after 30 days. Higher expressions of osteocalcin and osteopontin were observed in newly formed bone in the A-PRF group, compared to the control group. The bone formation ratio was also higher in the A-PRF group than in the control group. Thus, A-PRF application may result in enhanced new bone formation and may aid in accelerating bone formation. A-PRF was more rapid than a self-limiting process during induction of bone formation by enhancing osteoblast activity and may be useful for bone formation in clinical medicine.
Background and objectives: Preservation of bone after extraction is of prime importance. Good and sound bone present is useful for further prosthodontic rehabilitation or implant placement. Various bone grafts are available for restoring the continuity of bone which are either autogenic or allogenic. PRF being autogenous and drawn from individual's blood serves as a good substitute for bone reconstruction. The aim of this study is to assess the effects of PRF in extraction socket healing after tooth extraction. Materials and methods: Thirty patients of age group between 15-55 years visiting the dept of oral and maxillofacial surgery needing single tooth simple extractions were included in the study. PRF was prepared with blood drawn from individuals using standard technique. PRF was placed in extraction sockets followed by pressure application and suturing was done. Radiographic assessment of socket was done using IOPA. Ridge width was assessed using cast. The clinical follow up assessments were performed at 1, 4 and 12 weeks. Results: 18 males and 12 females were included in the study mean age of the patients was 35.43 years. The alveolar width for sockets were 11.33 ± 3.43 mm, 10.85 ± 3.23 mm and 10.45 ± 3.32 mm at 1, 4 and 12 weeks with loss in alveolar bone height being 0.4 ± 0.13 mm, 0.7 ± 0.17 mm and 0.8 ± 0.12 mm at 1, 4 and 12 weeks. The bone fill was measured by mean of gray level histogram values of IOPA of the extraction sockets obtained through Adobe Photoshop 7.0 software. It was 68.66 ± 2.36%, 80.34 ± 3.18% and 93.23 ± 2.07% at 1, 4 and 12 weeks respectively. Conclusion: Platelet-rich fibrin (PRF) placement after extraction procedure improves the bone width and bone height and quality of bone. The good amount of bone serves as an important precursor for future implant placement or prosthodontic rehabilitation of tooth.
The role of Platelet Rich Fibrin in bone defect regeneration
Egyptian Dental Journal, 2018
Background: Natural tissue regeneration relies on a cocktail of signaling molecules and growth factors. During natural wound healing, activated platelets concentrate in the wound area and secrete factors that play a role in wound healing. Platelet rich fibrin (PRF) represents a revolutionary step in the platelet gel therapeutic concept. It needs only centrifugation of the natural blood without additives. Objectives: This present study designed to evaluate the role of PRF on regeneration of bone defect. Design: In this study 24New Zealand white male rabbits were used with bilateral cortical bony defects in the submental mandibular area, divided into two groups. The right bone defects were filled with PRF (experimental) and the left bone defects kept empty (control). Each group was equally subdivided into three subgroups; rabbits were sacrificed at 2 nd , 4 th and 6 th weeks. Immunohistochemical analysis for collagen I and vascular endothelial growth factor (VEGF) were done. Results: The histological findings illustrated that all experimental subgroups showed more deposition of osteoid tissue and well organized bone trabeculae. Immunohistochemical findings of collagen I revealed statistically to be the highest in PRF group. While VEGF illustrated a positive expression by bone marrow stromal cells and bone tissue cells in different value and the PRF group recorded the highest value. Conclusion: It was concluded that PRF can accelerate bone regeneration and had effect on collagen I and VEGF expression.
Egyptian Dental Journal, 2019
OBJECTIVE: The purpose of this study was to compare the efficacy of Leucocyte-platelet-rich fibrin (L-PRF), and hydroxyapatite (HA) for reduction of pain and swelling, the incidence of dry socket, soft tissue healing, and bone regeneration after surgical removal of impacted mandibular third molar in human patients. Patients and Methods: Thirty-six patients (20 males and 16 females) requiring extraction of mandibular third molars were randomly grouped as L-PRF, HA, and Control-treated. All the procedures were done on an outpatient basis without any complication such as lip numbness. The patients were assessed for postoperative pain, swelling and maximal inter-incisal distance on the 1st, 3rd and 7th postoperative days. Exposed bony socket wall (dry socket), as well as soft tissue healing (healthy granulation tissue Formation), were also assessed at 1st, 3rd, 7th and 14th day of postoperative periods depending on the standard methods. Assessment of daily and total analgesics consumption was done and Radiological assessment of the extraction site was done at 2 and 6 months interval. Results: Pain, swelling, interincisal distance, and soft tissue healing had statistically significant more improvement for L-PRF group compared to HA and control groups. Radiographic assessment showed comparatively lesser bone density values in PRF and control sites at 2 and 6 months than HA site. Conclusion: Our study showed that L-PRF is better graft materials than HA regarding pain, swelling, dry socket, and soft tissue healing post-L-PRF placement in the extraction socket. Bone regeneration is induced quickly by HA as compared to PRF. However, study with a wide scale of clinical cases is very much essential to be more decisive regarding the efficacy of the graft materials.
Acta Medica Philippina
Background. Worldwide, periodontitis has the highest prevalence among the oral diseases. New medications are needed to repair the damage brought by periodontitis. Previous studies have been done to investigate agents such as antioxidants and platelet-rich fibrin (PRF) in oxidative stress. These studies focused on the role of antioxidants in periodontitis and other related systemic conditions. The aim of this study was to determine if antioxidants in combination with bone graft and PRF can increase the number of osteoblasts for new bone growth. Methods. Twenty-seven (27) Wistar rats were randomly allocated into 3 groups. In the first group, the postextraction tooth socket was filled with blood (control). In the second group, the socket was filled with antioxidants and bone graft. In the third group, the socket was filled with antioxidants, bone graft, and PRF. On day 14, the rats were euthanized and the tissue fixations were stained with hematoxylin-eosin (HE) for histologic examinat...