Interference of abnormal hemoglobins on the measurement of hemoglobin A1c by ion-exchange chromatography (original) (raw)

1990, Clinica Chimica Acta

is an accepted method for monitoring long-term blood glucose control in patients with diabetes mellitus [l]. Several methods for the measurement of Hb A,, have been developed, most are not specific ]1,21. Interference from Hb F [l-3], Hb S [1,4-61, Hb C [6] and by other abnormal hemoglobins [7,8] has been described. We report the effects of others abnormal hemoglobins on the Hb A,, results. The chromatograms obtained by high pressure liquid chromatography (HPLC) are shown. While others have studied diabetic patients with abnormal chromatographs or values [3,-5,8], we have examined five nondiabetic patients with abnormal hemoglobins. Therefore, this study is not influenced by an increased concentration of Hb A,, of unknown magnitude. Methods Measurements of Hb A,, Hb A,, was measured by liquid chromatography (LC) on minicolumns at 24.O"C by the Micro Column Test (BioRad, Hercules, CA). The reference interval is 4.3-5.9%. Hb A,, was also measured by HPLC by a modification of the.procedure published by G. Ellis et al. [9], as described by Engbaek et al. [2]. The reference interval is 4.4-6.4%. Hb A,, was also measured by enzyme immunoassay (EIA) [2]. The reference interval is 3.5-5.0s.