Lighting up individual DNA binding proteins with quantum dots (original) (raw)

The ability to determine the precise loci and occupancy of DNA-binding proteins is instrumental to our understanding of cellular processes like gene expression and regulation. We propose a single-molecule approach for the direct visualization of proteins bound to their template DNA. Fluorescent quantum dots (QD) are used to label proteins bound to DNA, allowing multicolor, nanomether-resolution localization. Protein���DNA complexes are linearly extended and imaged to determine the precise location of the protein binding sites ...