Regeneration of Shoot from Nodal explants of Cucumis sativus considering different Hormonal concentration (original) (raw)
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International Research Journal of Biological Sciences, 2015
Cucumber (Cucumis sativus), one of the most economically important vegetable (cucurbit) crops, is eaten raw or cooked and is popularly used as salads in south Asia. Considering the nutrition value of Cucumis sativus, the present investigation was undertaken to develop a rapid and efficient in vitro multiplication and regeneration system of this species using in vitro nodal explants. Addition of cytokinin is essential to induce shoot formation from the explants. Of the two cytokinins tested, BAP was more effective than kinetin at concentration of 1.5mg/l yielded best response (87%) on shoot formation. Kinetin at 1.5mg/l showed the highest shoot regeneration frequency (53%). When combination of auxin with IAA (0.5 mg/l) + BAP (1.0 -5.0 mg/l) and IAA (0.5 mg/l) + KN (1.0-5.0 mg/l) for shoots development, greater frequency (70%) were produced at IAA (0.5 mg/l) + BAP (3.0 mg/l) whereas IAA + KN were greater frequency (67%) at 3.0 mg/l KN. For root induction, four concentration of NAA were used. The maximum frequency of root formation (83%) was achieved within 3 week when isolated in vitro raised shoots were cultured on MS medium containing 0.5mg/l NAA.
Pure and Applied Biology
A proficient and speedy in vitro regeneration is a prerequisite for genetic modification of plants. The described protocol presents an efficient and practicable procedure for regeneration and multiple shoots induction by using shoot tips as explants for cucumber (Cucumis sativus L.). First, aseptic seedlings were established and shoot tips were excised from those seedlings. The cucumber seedlings used after 7 days of germination were found to be the best source of explants. Theses shoot tips were placed on MS medium supplemented with different concentrations of Benzyl amino purine (BAP) (0.5-2.5mg/L). It was observed that MS medium augmented with 1.5mg/L BAP resulted in maximum shoots formation. By increasing BAP beyond 1.5mg/L, rate of shoots formation was decreased. For rooting purpose, Indole Butyric Acid (IBA) (0.5-2.5mg/L) was used. Shoots greater than 2cm in size were shifted to rooting medium. Best roots development took place on MS medium having 1mg/L IBA. The plantlets with well-developed roots were shifted to peat moss containing pots and gradually acclimated to soil. This in vitro regeneration protocol can be used for genetic transformation of cucumber in future.
Genetika, 2014
The effect of different phytohormone concentrations on callusogenesis and organogenesis in two cucumber genotypes were studied. It was established that the rate of plant regeneration depends on genotype, explant type and culture medium. Hypocotyls were found to be more responsive than cotyledons in morphogenesis. In vitro planlet-regenerants have been obtained in hypocotyls explants on culture medium with 1.0 and 2.0 mgL-1 BA for cultivar Gergana and in 1.0 and 3.0 mgL-1K-line 15B. Induction of regeneration in cotyledons were established only in cultivar Gergana on culture medium supplemented with 3.0 mgL-1 BA and in combination of 0.5 mgL-1IAA.
Embryogenesis and plant regeneration from anther cultures of Cucumis sativus L
Scientia Horticulturae, 2003
The response of anthers to in vitro culture and effect of growth regulators, temperature pretreatment of anthers have been studied in Calypso and Green Long cultivars of cucumber (Cucumis sativus L.). Direct and callus mediated embryogenesis (embryogenic calli with embryos) was induced in Calypso and Green Long cultivars respectively on B5 medium (Gamborg's medium) supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and a-naphthaleneacetic acid (NAA) alone as well as 2,4-D in combination with N 6-benzylaminopurine/kinetin/thidiazuron (BAP/KN/TDZ). Optimal embryogenic calli/embryos were induced on B5 medium supplemented with 2.0 mM 2,4-D and 1.0 mM BAP. Temperature pretreatment of flower buds at 4 8C for 0-10 days and also at 32 8C for 1 day were tested and best response was obtained at 4 8C for 2 days. Embryo differentiation was achieved on B5 medium containing 0.09 M sucrose, 0.25 mM NAA and 0.25 mM KN. Embryo maturation was on B5 medium supplemented with 5.0 mM abscisic acid (ABA). Embryos germinated into plantlets on B5 medium containing 0.09 M sucrose. Plantlets were acclimatized in the controlled environment. In each cultivars, the root tips of 24 regenerated plantlets were analyzed for ploidy level, of which 21 and 17 have been haploids in Calypso and Green Long, respectively.
In Vitro plant regeneration of Cucumber (Cucumis sativum (L.) from cotyledon and hypocotyl explants
jsrr.in
In vitro plantlet regeneration has been obtained from 15-20days old seedling cotyledon and hypocotyl segments of Cucumis sativus (L.) were examined using various phytoharmons individually and in combination on Murashige and Skoog (MS) semi solid medium supplemented with BAP (1.0-5.0 mg/L) Kn (1.0-5.0 mg/L),IAA (0.5 mg/L)+ BAP (1.0-5.0 mg/L) and IAA (0.5 mg/L)+Kn (1.0-5.0 mg/L) for shoot proliferation IAA (0.5 mg/L)+BAP (3.0 mg/L) was proved to be best for induction of shoots for cotyledon and hypocotyl explants. All regenerated plantlets were rooted on MS medium supplemented with (1.0 mg/L) IAA the regenerated plants grew normally in the green house.
In vitro culture of Cucumis sativus L
Theoretical and Applied Genetics, 1989
The ability to regenerate plants from leaf explants has been tested for three highly inbred cucumber lines (B, G, S), their reciprocal hybrids, F 2 and BC 1 generations. The lines differed from each other in their regenerating ability, which was expressed by the percentage of explants regenerating embryoidal callus and mean number of plantlets per plant. Thus, the lines could be classified as frequently (B), intermediately (G) or occasionally regenerating ones (S). There were no reciprocal cross differences in the regeneration. It was found that the intermediately and intensively regenerating lines contain two pairs of dominant genes responsible for plant regeneration, characterized by complementary and probably additive interaction. The frequently regenerating line differed from the intermediately regenerating in the effect of one gene. It is supposed that the above-mentioned genes belong to three different loci. The ability to regenerate plants from leafexplants had high heritability.
Ecologia Balkanica, 2019
Development of an efficient in vitro plant regeneration system plays an important role for applying of biotechnological approaches for crop improvement. Therefore, the effect of added of BAP in germination phase on organogenesis of cotyledon and hypocotyl explants of two melon lines was investigated. The seeds germination medium contained three different concentrations of BAP (0.5, 1.0 and 1.5 mg L-1). The rate of plant regeneration was found to depend on genotype, explant type and culture medium. The cotyledons were more effective as explants for organogenesis and subsequent plant elongation than hypocotyls. Combination of 1.0 mg L-1 of BAP in germination medium and 0.5 mg L-1 BAP + 0.5 mg L-1 IAA in next regeneration medium gives the better regeneration answer in two explant types of line 11/9, while in line AGY the most effective was the combination of 1.5 mg L-1 of BAP and 0.5 mg L-1 BAP + 0.5 mg L-1 IAA. The experimental results indicated that pre-treatment with cytokinin BAP (...
Zenodo (CERN European Organization for Nuclear Research), 2023
Melon (Cucumis melo L.) is economically importance plants in agricultural production in the province of Balochistan, Pakistan. In this study, we developed various regeneration and transformation protocols and we examined the different earlier morphological characters of Cucumis melo on MS medium with different combinations and concentration of plant growth regulators (PGRs) (Auxin, cytokinin, and gibberellin). Result showed that the maximum value of callus was obtained when 0.50mgl-1 of BAP was combined with 0.50mgl-1 of 2,4-D about 52%. The maximum regeneration percentage was obtained by the combination of 1mgl-1 NAA, 0.5 mgl-1 BAP and 0 mgl-1 KIN about 80%. Although, the mean number of shoots were displayed a maximum value in MS medium having the combination of 2.5mgl-1 NAA, 1mgl-1 BAP, and 0mgl-1 KIN about 2.4± 0.3. In addition, when the treatment was treated with 1.5mgl-1 BAP, and 0.5mgl-1 IBA showed a significant response on morphological parameters of studied plants. Similarly, the maximum number of roots, root and shoot length was observed when treated with 2 mgl-1 of IAA and IBA respectively. We assume that statement of the research findings presented here lead to for further studies on in vitro propagation of melon plants.
Horticultural Biotechnology Research, 1970
In the present study, a competent and reproducible practice for the in vitro shoot regeneration of Cucurbita maxima, C. pepo and Cucumis sativus was developed from various explants through direct and indirect organogenesis. In C. maxima, between cotyledon and leaf segment, cotyledon was found to be most responsive for callus induction in MS medium augmented with 0.5 mg L-1 2,4 dichlorophenoxy acetic acid (2,4-D) plus 100 mg L-1 casein hydrolysate and 0.5 mg L-1 2,4-D plus 15% coconut water and for leaf segment it was on MS medium containing 2.5 mg L-1 2,4-D. Comparing the 2 explants it was found that leaf segment was most suitable for callus induction in C. maxima. For massive multiplication of C. pepo mericlones shoot tip and nodal cutting were used. MS medium containing 3.0 mg L-1 6-benzyl aminopurine plus 0.5 mg L-1 gibberellic acid (GA 3) was found most effective for shoot regeneration and 1.0 mg L-1 IBA was found most effective for rooting. In this trait cv. Bulum was more responsive than cv. Rumbo. On the other hand, to generate virus free plantlets of C. sativus, different concentrations of kinetin were used, and 1.5 mg L-1 KIN shown the best performance for primary culture establishment. For shoot multiplication, 1.0 mg L-1 BAP and 2.0 mg L-1 BAP plus 0.5 mg L-1 KIN containing medium shown best result. Subsequently, 2.0 mg L-1 BAP plus 0.5 mg L-1 KIN was best composition for root induction. Our report demonstrated comprehensive protocols and variability in explants, growth regulator response in shoot regeneration potential of in different cucurbit plants.