Mycoplasma species in cats with lower airway disease: improved detection and species identification using a polymerase chain reaction assay (original) (raw)
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Journal of Feline Medicine and Surgery, 2012
using a polymerase chain reaction assay species in cats with lower airway disease: improved detection and species identification Mycoplasma technique does not amount to an endorsement of its value or quality, or the claims made by its manufacturer. those of the authors and the inclusion in this publication of material relating to a particular product, method or of animals and interpretation of published materials lies with the veterinary practitioner. The opinions expressed are from actions or decisions based on information contained in this publication; ultimate responsibility for the treatment arising country. The authors, editors, owners and publishers do not accept any responsibility for any loss or damage advertising material, it is the responsibility of the reader to check that the product is authorised for use in their own bear this in mind and be aware of the prescribing laws pertaining to their own country. Likewise, in relation to Furthermore, drugs may be mentioned that are licensed for human use, and not for veterinary use. Readers need to formulations that are not available or licensed in the individual reader's own country.
Hemotropic Mycoplasmas in Stray Cats… Hosseini Hooshyar S, et al
2016
Article type: Original Article Background: Feline haemotropic mycoplasma are a group of pleomorphic bacteria causing hemolytic anemia along with anorexia, lethargy, dehydration, weight loss and in many cases sudden death in infected animal. However, there is a limited data on the prevalence of these organisms in Iranian cats. Methods: We investigated the presence of feline haemotropic mycoplasma and probable risk factors for these infections among 60 ectoparasite-infested stray cats in southeast of Iran using PCR assay. Results: The overall prevalence of haemotropic mycoplasma was estimated 18.3%. Pallor mucous membrane, anorexia, weight loss and splenomegaly were the most common signs and the infection rate was significantly higher in symptomatic cats in comparison with apparently healthy ones (P = 0.001). Age, gender and hematological alterations were not significantly associated with infection status while the level of BUN, creatinine, total protein and globulin were significantl...
Isolation and Identification of Mycoplasma Species in Dogs
Agriculture Journal IJOEAR, 2020
Mycoplasmas can be associated with several canine health issues, mainly when dogs do not respond to antimicrobial treatment usually aimed at bacterial infections. Different mycoplasma species can be found in both healthy and sick animals; however, the following subjects have yet to be fully understood: The role played by mycoplasmas in canine habitats and the various diseases caused by them. The aim of the present study is to assess the presence of mycoplasma in dog samples at NUDMIC/UFF, RJ, Brazil, over a timeframe of ten years. Out of all assessed dogs, 9.67% (15/155) had respiratory symptoms, whereas the rest of them were asymptomatic. Moreover, 29.96% of the cultured samples (77/257) were positive for mycoplasmas. Typical colonies of said samples were divided into 42.86% (33/77) of oropharynx samples, 51.95% (40/77) of urogenital samples and 5.19% (4/77) of samples from other sources. Species Mycoplasma canis, Mycoplasma edwardii and Mycoplasma cynos were identified by PCR and/or immunoperoxidase. The most common species was M. canis. M. cynos was found in a dog with signs of respiratory disease. Despite the recent improvement in early identification and the biomolecular knowledge surrounding canine mycoplasma, the etiopathogenesis of canine mycoplasmosis remains uncertain..
Journal of Feline Medicine and Surgery, 2013
The diagnosis of feline haemoplasmosis has improved over the years, with several techniques enabling a clear and specific diagnosis, and where polymerase chain reaction (PCR) is considered as the ‘gold standard’. The aim of this study was to survey the prevalence of feline haemoplasmas in 320 cats from the north-central region of Portugal by the use of real-time PCR, as well as to evaluate any associations between infection, clinical presentation and risk factors. The overall prevalence of infection by feline haemoplasmas was 43.43% (139/320), where 41.56% (133/320) corresponded to Candidatus Mycoplasma haemominutum ( CMhm), 12.81% (41/320) to Mycoplasma haemofelis ( Mhf), 4.38% (14/320) to Candidatus Mycoplasma haematoparvum and 1.25% (4/320) to Candidatus Mycoplasma turicensis. Almost 13% (47/320) of the samples were co-infected, with the most common co-infection being CMhm and Mhf (23.74%). Infection was found statistically significant with feline immunodeficiency/feline leukaemi...
Pilot study to evaluate the role of Mycoplasma species in cat bite abscesses
Journal of Feline Medicine and Surgery, 2014
Cat bite cellulitis and abscesses are common in cats and are among the most common infectious diseases presented to veterinary practices each year. 1 The penetrating wounds induced by the feline canine teeth inoculate high numbers of microbes into the subcutaneous tissues, while providing crush injury that results in tissue devitalization. The wounds are predominately closed immediately after the canine tooth exits, and even the most delicate microbes can thrive in this environment. Abscesses often develop and are generally treated with surgical drainage, with or without antibiotic therapy.
Infections of cats with blood mycoplasmas in various contexts
Acta Veterinaria Brno, 2021
Haemotropic microorganisms are the most common bacteria that infect erythrocytes and are associated with anaemia of varying severity. The aim of this study was to focus on the occurrence of Mycoplasma haemofelis, Mycoplasma haemominutum, and Mycoplasma turicensis in cats. We followed infected individuals’ breeding conditions, age, sex, basic haematological indices, and co-infection with one of the feline retroviruses. A total of 73 cats were investigated. Haemoplasmas were detected by PCR and verified by sequencing. Haematology examination was performed focusing on the number of erythrocytes, haemoglobin concentrations and haematocrit. A subset of 40 cat blood samples was examined by a rapid immunochromatography test to detect retroviruses. The following was found in our study group: M. haemofelis in 12.3% of individuals, M. haemominutum in 35.6% of individuals and M. turicensis in 17.8% of individuals. A highly significant difference was found between positive evidence of blood myc...
Prevalence of hemotropic mycoplasmas in healthy and unhealthy cats and dogs in Spain
Journal of veterinary diagnostic investigation: official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
The aims of the present study were to determine the prevalence of hemoplasmas in cats and dogs from the Barcelona area of Spain with the use of species-specific quantitative polymerase chain reaction (qPCR) assays and to evaluate any associations between hemoplasma infection, clinical presentation, and vector-borne infections. Blood samples from cats (191) and dogs (182) were included and were classified as healthy (149) or unhealthy (224). Ethylenediamine tetra-acetic acid blood samples underwent DNA extraction and qPCR analysis. Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum', and 'Candidatus Mycoplasma turicensis' were detected in cats, whereas Mycoplasma haemocanis and 'Candidatus Mycoplasma haematoparvum' were detected in dogs, with prevalences of 3.7%, 9.9%, 0.5%, 14.3%, and 0.6%, respectively. In cats, no association between hemoplasma infection and health status, age, breed, presence of anemia, Feline leukemia virus status, and other vecto...
Prevalence of feline haemotropic mycoplasmas in convenience samples of cats in Germany
Journal of Feline Medicine and Surgery, 2008
The aim of this prospective study was to evaluate the prevalence of feline haemotropic mycoplasmas in Germany, to determine probable risk factors for these infections and to compare the diagnostic value of microscopic examination of blood smears to polymerase chain reaction (PCR). For the prevalence study, convenience samples (Ethylene diamine-tetraacetic acid (EDTA) blood) from 262 (64.5% male and 35.5% female) cats were included. A PCR for the detection of Mycoplasma haemofelis (MHF) and ‘ Candidatus Mycoplasma haemominutum’ (CMH) as well as a feline leukaemia virus (FeLV)/feline immunodeficiency virus (FIV) enzyme-linked immunoassay was performed. Blood smears from 224 cats were examined and the sensitivity and specificity of the microscopic diagnosis were determined. The prevalence of CMH, MHF, and CMH/MHF co-infection was 22.5%, 4.5%, and 0.8%, respectively. CMH was significantly associated with male gender ( P=0.047), older age ( P=0.0015) and both FeLV ( P=0.002) and FIV infe...