Prevalence, Phylogroups and Antimicrobial Susceptibility of Escherichia coli Isolates from Food Products (original) (raw)
Related papers
2021
The emergence of multi-drug resistant E. coli is an important matter of increasing considerable concern to global public health. The aim of this study was to investigate the incidence, antibiotic resistance pattern, phylogroups and genetic variation of E. coli isolates from raw milk, vegetable salad and ground meat samples. Methods: Culture-based techniques, Kirby-Bauer disk diffusion susceptibility testing, PCR and RAPD assays were used to determine the incidence rate, antimicrobial resistance pattern, phylogenetic groups and genetic diversity of the E. coli isolates. Results: E. coli isolates were highly resistant to amoxicillin (79.16%), trime-thoprim-sulfamethoxazole (70.83%), amoxicillin-clavulanic acid (62.50%), tetracycline (54.16%), chloramphenicol (54.16%), nitrofurantoin (54.16%), ampicillin (45.83%), streptomycin (45.83%), and kanamycin (33.33%); and completely susceptible to norfloxacin and azithromycin. 70.83% of the isolates were multi-drug resistant. Most E. coli isol...
Microbial Drug Resistance, 2002
Escherichia coli is known as one of the most important foodborne pathogens in humans, and contaminated chicken meat is an important source of foodborne infection with this bacterium. The occurrence of extended-spectrum β-lactamase (ESBL)-producing E. coli (ESBL-Ec), in particular, in chicken meat is considered a global health problem. This study aimed to determine the magnitude of E. coli, with special emphasis on ESBL-Ec, along with their phenotypic antimicrobial resistance pattern in frozen chicken meat. The study also focused on the determination of ESBL-encoding genes in E. coli. A total of 113 frozen chicken meat samples were purchased from 40 outlets of nine branded supershops in five megacities in Bangladesh. Isolation and identification of E. coli were done based on cultural and biochemical properties, as well as PCR assay. The resistance pattern was determined by the disc diffusion method. ESBL-encoding genes were determined by multiplex PCR. The results showed that 76.1% of samples were positive for E. coli, of which 86% were ESBL producers. All the isolates were multidrug-resistant (MDR). Resistance to 9-11 and 12-13 antimicrobial classes was observed in 38.4% and 17.4% isolates, respectively, while only 11.6% were resistant to 3-5 classes. Possible extensive drug resistance (pXDR) was found in 2.3% of isolates. High single resistance was observed for oxytetracycline (93%) and amoxicillin (91.9%), followed by ampicillin (89.5%), trimethoprim-sulfamethoxazole, and pefloxacin (88.4%), and tetracycline (84.9%). Most importantly, 89.6% of isolates were resistant to carbapenems. All the isolates were positive for the blaTEM gene. However, the blaSHV and blaCTX-M-2 genes were identified in two ESBL-non producer isolates. None of the isolates carried the blaCTX-M-1 gene. This study provided evidence of the existence of MDR and pXDR ESBL-Ec in frozen chicken meat in Bangladesh, which may pose a risk to human health if the meat is not properly cooked or pickled raw only. This emphasizes the importance of the implementation of good slaughtering and processing practices by the processors.
Annals of Clinical Microbiology and Antimicrobials
Background: Consumption of meat contaminated by E. coli causes a serious illness and even death to affected individuals. Recently the emerging of antibiotic resistant foodborne E. coli poses serious public health risks worldwide. However, little is known about the antibiotic resistance profile of E. coli in Ethiopia. This study aimed to determine the prevalence and Antimicrobial resistance (AMR) status of E. coli isolated from different type of meat. Methods: Overall 292 samples were collected from December 2015 to April 2016 from slaughterhouses to determine the prevalence and AMR of E. coli isolated from raw beef, mutton, chevon and chicken meat from Addis Ababa and Bishoftu, Ethiopia. The isolates were screened for AMR against commonly used antibiotics circulating in the Ethiopian market. Both phenotypic and genotypic approach were employed for AMR detection using disc diffusion test and PCR respectively. Results: The prevalence of E. coli was 63 (21.6%), indicating one sample in every five samples harbors E. coli. Among these, the highest E. coli isolates was observed in chicken meat samples (37.0%; 27), followed by mutton (23.3%; 17), chevon (20.6%; 15) and beef (5.5%; 4). Results of disk diffusion test on the 63 isolates showed that only 4.8% of them were not resistance to all antimicrobials tested. Multiple drug resistance (resistance to ≥3 drugs) was 46.0%. Significantly high resistance to ampicillin (71.4%) and tetracycline (47.6%) was observed. Identification of genes associated with AMR was also done using PCR. The prevalence of E. coli isolates harboring resistance gene responsible for tetracycline (tet(A)), beta lactams (blaCMY) and sulphanamide (sulI) antibiotics were found 65.1, 65.1 and 54.0%, respectively. Twenty-five out of the 63 (39.7% %) E. coli isolates have got antimicrobial resistance gene to three or more classes of drugs. The associations of antimicrobial resistance phenotypes and resistance genes was also determined. The detection of resistance trait against tetracycline, sulphametazole and chloramphenicol measured either phenotypically or genotypically were high. Conclusions: The rising levels of resistance E. coli to multiple antimicrobial dictate the urgent need to regulate and monitor antimicrobial use in both animals and humans.
Bangladesh Journal of Scientific and Industrial Research, 2023
This study aimed to identify and evaluate the occurrence and antibiotic susceptibility of E. coli in various dairy products. Physical, biochemical, and molecular tests were used to identify and characterize the E. coli isolates. The study found that E. coli was present in 16% of raw milk, 8% of cheese, 6% of butter, and 10% of ice cream samples. No E. coli O157:H7 or its toxin stx1 was identified in any samples. The antibiotic susceptibility test revealed that the highest susceptibility was to Azithromycin, Gentamycin, and Ciprofloxacin, while the lowest susceptibility was to Amoxicillin/clavulanic acid, Tetracycline, and Trimethoprim/sulfamethoxazole. Interestingly, 36.84% of E. coli isolates showed multidrug resistance, which is a serious health concern as they may transmit and develop antibiotic resistance in the human body. The study highlights the need for continued surveillance and monitoring of dairy products for food safety and public health purposes.
Antibiotic Resistance in Escherichia coli isolated from chicken meat in Taif region (Saudi Arabia)
2010
Isolation of multidrug resistant Escherichia coli from diseased livestock is becoming rampart from samples submitted for disease diagnostic purposes in some Tertiary Veterinary Teaching Hospitals in Nigeria. In order to evaluate the possible roles of commensal E. coli from apparently healthy animals in the epidemiology of drug resistant pathogens, antibiotic resistance status of 240 E. coli isolated from 300 rectal swab from apparently healthy cattle from a major cattle market in Ibadan, Oyo State, South West Nigeria (a location very close to two of the Veterinary Teaching Hospitals), were grown aerobically at breakpoint concentration for ciprofloxacin, cefepime, chloramphenicol, tetracycline, ampicillin, kanamycin, streptomycin and nalidixic acid (all obtained from SIGMA-ALDRICH) according to standard method by Clinical Laboratory Standards Institute. A very high level of resistance was observed in all the antibiotics studied, with the highest resistance of 97% for kanamycin and 96
Proceedings of the Zoological Society, 2021
Foodborne diseases associated with fresh produce consumption have escalated worldwide, causing microbial safety of produce of critical importance. Bacteria that have increasingly been detected in fresh produce are Escherichia coli and Salmonella spp., both of which have been shown to progressively display antimicrobial resistance. The study focused on the assessment of antimicrobial resistance of these enteric bacteria from different kinds of fresh produce from various open air markets and supermarkets in the Philippines. Using the disk diffusion assay on a total of 50 bacterial isolates obtained from 410 fresh produce surveyed, monoresistance to tetracycline was observed to be the most prevalent (38%), followed by multidrug resistance to tetracycline, chloramphenicol, ciprofloxacin, and nalidixic acid (4%), and lastly by dual resistance to tetracycline and chloramphenicol (2%). Using multiplex and simplex polymerase chain reaction (PCR) assays, tetA (75%) and tetB (9%) were found in tetracycline resistant isolates, whereas catI (67%) and catIII (33%) were detected in chloramphenicol resistant isolates. Sequence analysis of gyr and par genes from the ciprofloxacin and nalidixic acid resistant isolates revealed different mutations. Based on the results, fresh produce act as a reservoir of these antibiotic resistant bacteria which may pose health threat to consumers.
The present study was carried out to screen and analyze the genetic characteristics of antibiotic resistance in Escherichia coli strains isolated from chicken meat marketed in the local markets of the Taif region in Saudi Arabia. A total of 119 samples were purchased from various supermarkets and examined for bacterial contamination with resistant E. coli. Thirty-seven E. coli isolates were evaluated for their antibiotic susceptibilities and the presence of class 1 integrons and antibiotic resistance genes. Results of antibiograms revealed that E. coli isolates were resistant to one or more of the antibiotics tested. Resistance was most frequently observed against sulphafurazole (89.2%), ampicillin (78.4%), nalidixic acid (70.3%), streptomycin (48.6%), chloramphenicol (32.4%), and gentamicin (24.3%). Fifteen E. coli strains have multidrug resistance phenotypes and harbored at least three antibiotic resistance genes. The bla TEM (b-lactamase) and sul (sulfonamide) resistance encoding genes were detected in all the tested isolates. Polymerase chain reaction screening detected class 1 integrons in all multiresistant E. coli isolates. The present study provides an assessment of the occurrence of multidrug resistance of E. coli from raw chicken meat collected from local markets.
In Bangladesh, Escherichia coli strains show a wide range of antibiotic resistance, due to uncontrolled antibiotic use in animals and insufficient surveillance, which poses serious public health risks and potentially fatal outcomes. This study aimed to identify antimicrobial resistance patterns and the prevalence of antibiotic resistance genes in Escherichia coli isolated from raw meat sold in retail outlets across the Rajshahi division of Bangladesh. Methods: 92 raw meat samples (broiler chicken: 20, layer chicken: 20, cattle: 20, goat: 20, buffalo: 12) were collected from four districts in Rajshahi division of Bangladesh. Phenotypic identification was done by using different cultural characteristics and biochemical tests and, the Kirby-Bauer disc diffusion method was used to detect antibiotic susceptibility of Escherichia coli. Molecular conformation and detection of antibiotic resistance genes were performed by PCR analysis. Results: In phenotypic detection, the prevalence of Escherichia coli in raw meat was 25%, with varying rates across types: cattle meat (25%), goat meat (10%), buffalo meat (8.33%), broiler chicken meat (45%), and layer chicken meat (30%). Ceftriaxone, sulphonamide, enrofloxacin, gentamycin, ciprofloxacin, levofloxacin, neomycin, and colistin sulfate showed sensitivity ranging from 52.17% to 100%. Whereas, penicillin, tetracycline, and oxytetracycline showed no sensitivity. Conversely, erythromycin, doxycycline, tetracycline, amoxicillin, ampicillin, and penicillin all exhibited resistance in 47.83% to 100%. In addition, levofloxacin, neomycin, and colistin sulfate all showed no resistance. The study found the prevalence of antibiotic resistance genes: streptomycin (aadA1) 4.35%, erythromycin (ereA) 4.35%, gentamicin (aac(3)-IV) 8.70%, tetracycline (tetB) 17.39%, sulfonamide (sul1) 21.80%, and tetracycline (tetA) 43.48%. No tetC, bla TEM , bla SHV , or bla CMY was detected. Conclusion: Detection of antibiotic resistant genes highlights contamination complexity, urging continuous research and monitoring measures to ensure public health safety across the food supply chain.