Microfluidic Chromatographic Techniques (original) (raw)
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Recent Advances in Microfluidics-Based Chromatography—A Mini Review
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Microfluidics-based liquid chromatography is based on the miniaturization of the different types of liquid chromatography (LC) systems (e.g., affinity, adsorption, size exclusion, ion exchange) on a microchip to perform on-chip separation of different types of analytes. On-chip chromatography finds applications in genomics, proteomics, biomarker discovery, and environmental analysis. Microfluidics-based chromatography has good reproducibility and small sample consumption. However, the on-chip chromatography fabrication techniques are often more challenging to perform than conventional LC column preparation. Different research groups have attempted to develop different techniques to fabricate microfluidics-based LC systems. In this review, we will summarize the recent advances in microfluidics-based chromatography.
Design Techniques for Microfluidic Devices Implementation Applicable to Chemical Analysis Systems
Process Analysis, Design, and Intensification in Microfluidics and Chemical Engineering, 2019
This chapter provides a guide for microfluidic devices development and optimization focused on chemical analysis applications, which includes medicine, biology, chemistry, and environmental monitoring, showing high-level performance associated with a specific functionality. Examples are chemical analysis, solid phase extraction, chromatography, immunoassay analysis, protein and DNA separation, cell sorting and manipulation, cellular biology, and mass spectrometry. In this chapter, most information is related to microfluidic devices design and fabrication used to perform several steps concerning chemical analysis, process preparation of reagents, samples reaction and detection, regarding water quality monitoring. These steps are especially relevant to lab-on-chip (LOC) and micro-total-analysis-systems (μTAS). μTAS devices are developed in order to simplify analytical chemist work, incorporating several analytical procedures into flow systems. In the case of miniaturized devices, the ...
Microfluidics Era in Chemistry Field: A Review
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By miniaturizing the reactor dimension, microfluidic devices are attracting world attention and starting the microfluidic era, especially in the chemistry field because they offer great advantages such as rapid processes, small amount of the required reagents, low risk, ease and accurate control, portable and possibility of online monitoring. Because of that, microfluidic devices have been massively investigated and applied for the real application of human life. This review summarizes the up-to-date microfluidic research works including continuous-flow, droplet-based, open-system, paper-based and digital microfluidic devices. The brief fabrication technique of those microfluidic devices, as well as their potential application for particles separation, solvent extraction, nanoparticle fabrication, qualitative and quantitative analysis, environmental monitoring, drug delivery, biochemical assay and so on, are discussed. Recent perspectives of the microfluidics as a lab-on-chip or mic...
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We report a micromilled, pressure-resistant mixing chip for application in on-line comprehensive two-dimensional liquid chromatography (LC×LC). This microfluidic mixer is based on chaotic advection generated in grooved microchannels, and can be operated at flow rates compatible with LC×LC (0.1-1 mL/min). The design of this chip was optimized and tested in our previous work in the transparent and flexible silicon rubber material, polydimethylsiloxane (PDMS). In this work, the microfluidic chip was micromilled in rigid cyclic-olefin copolymer (COC) substrate in order to better withstand the high pressure environment of LC×LC. Two micromilled parts were bonded using solvent-vapour-assisted bonding under elevated temperature and pressure. A specially designed, robust, low-deadvolume interface allows direct connection of the microfluidic chip to an LC×LC system using standardized HPLC connectors. Thus-fabricated chips can withstand pressures of 200 bar. The chip was successfully implemen...
Flexible fabrication, packaging, and detection approach for microscale chromatography systems
Sensors and Actuators B: Chemical, 2009
This paper describes a simple manufacturing and packaging method to fabricate microfluidic channels and obtain an optical detection interface for rapid prototyping of microscale chemical and biological analysis systems. Specifically, this work reports on efforts to develop new methods for simple, fast and reliable design and fabrication of microscale field flow fractionation (FFF) based biological analysis systems and a compatible optical detector flow cell. First, a rapid prototyping technique is used to realize electrical FFF microchannels and second, a modular detector interface with a 38 nl detection region is employed, which when connected to an appropriate microfluidic channel, results in a ElFFF prototype. Detector characterization is carried out to evaluate important figures of merit such as sensitivity, S/N ratio and limit of detection. Plate height, a primary performance metric for field flow fractionation is determined and compared with an on-chip detector. Separation of a multicomponent mixture of polystyrene particles is also demonstrated. The developed system allows for an easy plug-and-play approach to the optical interface and has resulted in improved operational flexibility and robustness of the microsystem. in biomedical engineering from the Louisiana Tech University and PhD in bioengineering from the University of Utah. His interests include micro/nanofabrication for separation systems, detectors, haptic devices, drug delivery devices.
Partition-induced vector chromatography in microfluidic devices
Journal of Colloid and Interface Science, 2011
The transport of Brownian particles in a slit geometry in the presence of an arbitrary two-dimensional periodic energy landscape and driven by an external force or convected by a flow field is investigated by means of macrotransport theory. Analytical expressions for the probability distribution and the average migration angle of the particles are obtained under the Fick-Jackobs approximation. The migration angle is shown to differ from the orientation angle of the driving field and to strongly depend on the physical properties of the suspended species, thus providing the basis for vector chormatography, in which different species move in different directions and can be continuously fractionated. The potential of microfluidic devices as a platform for partition-induced vector chromatography is demonstrated by considering the particular case of a piece-wise constant, periodic potential that, in equilibrium, induces the spontaneous partition of different species into high and low concentration stripes, and which can be easily fabricated by patterning physically or chemically one of the surfaces of a channel. The feasibility to separate different particles of the same and different size is shown for systems in which partition is induced via 1g-gravity and Van der Waals interactions in physically and chemically patterned channels, respectively.
Emerging Trends in Microfluidics Based Devices
Biotechnology Journal, 2020
The major challenge for scientists and engineers today is to develop technologies for the improvement of human health in both developed and developing countries. However, the need for cost-effective, high-performance diagnostic techniques is very crucial for providing accessible, affordable, and high-quality healthcare devices. In this context, microfluidicbased devices (MFDs) offer a powerful platform for automation and integration of complex operations onto a single chip. The distinct advantage of MFDs lies in precise control of the sample quantities and flow rate of samples and reagents that enable quantification and detection of analytes with high resolution and sensitivity. With these excellent properties, Microfluidics (MFs) has been used for various applications in healthcare, along with other biological and medical areas. This review focuses on the emerging demands of MFs in different fields, i.e., biomedical diagnostics, environmental analysis, food and agriculture research, etc. in the last about three years. It also aims to reveal new opportunities in these areas and future prospects of commercial MFDs.